An efficient refolding method for the preparation of recombinant human prethrombin-2 and characterization of the recombinant-derived α-thrombin

Journal of Biochemistry

Volumn 130, Issue 2, 2001, Pages 269-277

  Soejima, Kenji ^a   Mimura, Noriko ^a   Yonemura, Hiroshi ^a   Nakatake, Hiroshi ^a   Imamura, Takayuki ^a   Nozaki, Chikateru ^a  

^a Chemo Sero Therapeutic Research Institute   (Japan)

Author keywords

Inclusion body; Prethrombin 2; Recombinant; Refolding; thrombin

Indexed keywords

FIBRINOGEN; FIBRINOPEPTIDE; PRETHROMBIN 2; RECOMBINANT PROTEIN; THROMBIN; THROMBIN DERIVATIVE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN PURIFICATION;

ANIMALS; ENZYME PRECURSORS; GENETIC VECTORS; HUMANS; INCLUSION BODIES; PROTEIN FOLDING; PROTHROMBIN; RECOMBINANT PROTEINS; THROMBIN;

ECHIS CARINATUS; ESCHERICHIA COLI; SERPENTES;

EID: 0034874544     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002982     Document Type: Article

References (38)

1. Blood coagulation
2. Cofactor proteins in the assembly and expression of blood clotting enzyme complexes
3. The coagulation cascade: Initiation, maintenance, and regulation
4. Prothrombin and thrombin
5. Prothrombin
6. Cloning and expression of human prethrombin 2 cDNA in Escherichia coli
7. Purification and activation of recombinant human prethrombin 2 produced in E. coli
8. Expression and folding of recombinant bovine prethrombin-2 and its activation to thrombin
9. The glycosylation sites and structural characteristics of oligosaccharides on recombinant human thrombomodulin
10. Increased anticoagulant activity of recombinant thrombomodulin modified with glycosaminoglycan
11. Effect of protein C and activated protein C on coagulation and fibrinolysis in normal human subjects
12. Expression of completely gamma-carboxylated recombinant human prothrombin
13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
14. Immobilized hirudin and hirudin-based peptides used for the purification of recombinant human thrombin prepared from recombinant human prothrombin
15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
16. Comparison of the esterase activities of trypsin, plasmin, and thrombin on guanidinobenzoate esters. Titration of the enzymes
17. Quantifying thrombin-catalyzed release of fibrinopeptides from fibrinogen using high-performance liquid chromatography
18. An improved spectrophotometric assay for human plasma carboxypeptidase N1
19. Human thrombins. Production, evaluation, and properties of alphathrombin
20. Prothrombin activator from Echis carinatus venom
21. Demonstration of peptide:N-glycosidase F activity in endo-beta-N-acetylglucosaminidase F preparations
22. Human prothrombin activation
23. Specificity of thrombin and its action on fibrinogen
24. Amino acid sequence studies on factor XIII and the peptide released during its activation by thrombin
25. Activation of the fibrin stabilizing factor of plasma by thrombin
26. Thrombin-catalyzed activation of single chain bovine factor V
27. The effect of thrombin on human factor VIII. Cleavage of the factor VIII procoagulant protein during activation
28. Factor XI activation in a revised model of blood coagulation
29. TAFI, or plasma procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex
30. Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C
31. Thrombomodulin as a model of molecular mechanisms that modulate protease specificity and function at the vessel surface
32. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation
33. The purification and mechanism of action of human antithrombin-heparin cofactor
34. Heparin cofactor II. Purification and properties of a heparin-dependent inhibitor of thrombin in human plasma
35. Refined structure of the hirudin-thrombin complex
36. Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases
37. Comparative analysis of haemostatic proteinases: Structural aspects of thrombin, factor Xa, factor IXa and protein C