A direct comparison of whole cell patch and sharp electrodes by simultaneous recording from single spinal neurons in frog tadpoles

Journal of Neurophysiology

Volumn 92, Issue 1, 2004, Pages 380-386

  Li, W C ^a   Soffe, S R ^a   Roberts, Alan ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTION POTENTIAL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTRODE; INTERMETHOD COMPARISON; MEMBRANE STEADY POTENTIAL; NERVE CONDUCTION; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; SENSORY STIMULATION; SHARP INTRACELLULAR ELECTRODE; SPIKE; SPINAL CORD NERVE CELL; SWIMMING; TADPOLE; WHOLE CELL PATCH ELECTRODE; XENOPUS;

ACTION POTENTIALS; ANIMALS; ELECTRODES; LARVA; PATCH-CLAMP TECHNIQUES; SPINAL NERVES; XENOPUS;

EID: 3042643080     PISSN: 00223077     EISSN: None     Source Type: Journal    
DOI: 10.1152/jn.01238.2003     Document Type: Article

References (21)

1. Aiken SP, Kuenzi FM, and Dale N. Xenopus embryonic spinal neurons recorded in situ with patch-clamp electrodes-conditional oscillators after all? Eur J Neurosci 18: 333-343, 2003.
2. Clarke JDW and Roberts A. Interneurones in the Xenopus embryo spinal cord: sensory excitation and activity during swimming. J Physiol 354: 345-362, 1984.
3. Cymbalyuk GS, Gaudry Q, Masino MA, and Calabrese RL. Bursting in leech heart interneurons: cell-autonomous and network-based mechanisms. J Neurosci 22: 10580-10592, 2002.
4. Dale N. The isolation and identification of spinal neurons that control movement in the Xenopus embryo. Eur J Neurosci 3: 1025-1035, 1991.
5. Dale N. Experimentally derived model for the locomotor pattern generator in the Xenopus embryo. J Physiol 489: 489-510, 1995.
6. Dale N and Kuenzi FM. Ion channels and the control of swimming in the Xenopus embryo. Prog Neurobiol 53: 729-756, 1997.
7. Fenwick EM, Marty A, and Neher E. A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine. J Physiol 331: 577-597, 1982.
8. Hamill OP, Marty A, Neher E, Sakmann B, and Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch 391: 85-100, 1981.
9. Ince C, van Bavel E, van Duijn B, Donkersloot K, Coremans A, Ypey DL, and Verveen AA. Intracellular microelectrode measurements in small cells evaluated with the patch clamp technique. Biophys J 50: 1203-1209, 1986.
10. Jack JJB. An introduction to linear cable theory. In: The Neurosciences: Fourth Study Program, edited by Schmitt FO and Worden FG. Cambridge, MA: MIT Press, 1979, p. 423-437.
11. Li WC, Soffe SR, and Roberts A. Spinal inhibitory neurons that modulate cutaneous sensory pathways during locomotion in a simple vertebrate. J Neurosci 22: 10924-10934, 2002.
12. Li WC, Soffe SR, and Roberts A. The spinal interneurons and properties of glutamatergic synapses in a primitive vertebrate cutaneous flexion reflex. J Neurosci 23: 9068-9077, 2003.
13. Pongracz F, Firestein S, and Shepherd GM. Electrotonic structure of olfactory sensory neurons analyzed by intracellular and whole cell patch techniques. J Neurophysiol 65: 747-758, 1991.
14. Roberts A and Sillar KT. Characterization and function of spinal excitatory interneurons with commissural projections in Xenopus laevis embryos. Eur J Neurosci 2: 1051-1062, 1990.
15. Sillar KT and Roberts A. Unmyelinated cutaneous afferent neurons activate two types of excitatory amino acid receptor in the spinal cord of Xenopus laevis embryos. J Neurosci 8: 1350-1360, 1988.
16. Soffe SR. Active and passive membrane properties of spinal cord neurons that are rhythmically active during swimming in Xenopus embryos. Eur J Neurosci 2: 1-10, 1990.
17. Soffe SR and Roberts A. Tonic and phasic synaptic input to spinal cord motoneurons during fictive locomotion in frog embryos. J Neurophysiol 48: 1279-1288, 1982.
18. Staley KJ, Otis TS, and Mody I. Membrane properties of dentate gyrus granule cells: comparison of sharp microelectrode and whole-cell recordings. J Neurophysiol 67: 1346-1358, 1992.
19. Svirskis G, Gutman A, and Hounsgaard J. Detection of a membrane shunt by DC field polarization during intracellular and whole cell recording. J Neurophysiol 77: 579-586, 1997.
20. Thurbon D, Luscher HR, Hofstetter T, and Redman SJ. Passive electrical properties of ventral horn neurons in rat spinal cord slices. J Neurophysiol 79: 2485-2502, 1998.
21. Verheugen JA, Fricker D, and Miles R. Noninvasive measurements of the membrane potential and GABAergic action in hippocampal interneurons. J Neurosci 19: 2546-2555, 1999.