High-level expression and single-step purification of leucyl-tRNA synthetase from Aquifex aeolicus

Protein Expression and Purification

Volumn 36, Issue 1, 2004, Pages 146-149

  Ling, Chen ^a   Zheng, Yong Gang ^a   Wang, En Duo ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Aquifex aeolicus; argU; Escherichia coli; Leucyl tRNA synthetase; Overproduction

Indexed keywords

BACTERIAL PROTEIN; HISTIDINE; LEUCINE TRANSFER RNA LIGASE; RECOMBINANT PROTEIN;

AMINOACYLATION; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; KINETICS; MOLECULAR CLONING;

BACTERIAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; HISTIDINE; KINETICS; LEUCINE-TRNA LIGASE; RECOMBINANT PROTEINS; TRANSFER RNA AMINOACYLATION;

AQUIFEX AEOLICUS; ESCHERICHIA COLI;

EID: 3042598416     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.04.003     Document Type: Article

References (14)

1. Ibba M., Soll D. Aminoacyl-tRNA synthesis. Annu. Rev. Biochem. 69:2000;617-650
2. Eriani G., Delarue M., Poch O., Gangloff J., Moras D. Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs. Nature. 347:1990;203-206
3. Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature. 392:1998;353-358
4. Gouda M., Yokogawa T., Asahara H., Nishikawa K. Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure. FEBS Lett. 518:2002;139-143
5. Gouda M., Yokogawa T., Nishikawa K. The β subunit of Aquifex aeolicus leucyl-tRNA synthetase is responsible for cognate tRNA recognition. Biochem. Biophys. Res. Commun. 297:2002;950-955
6. Xu M.G., Chen J.F., Martin F., Zhao M.W., Eriani G., Wang E.D. Leucyl-tRNA synthetase consisting of two subunits from hyperthermophilic bacteria Aquifex aeolicus. J. Biol. Chem. 277:2002;41590-41596
7. Zhao M.W., Hao R., Chen J.F., Martin F., Eriani G., Wang E.D. Enzymes assembled from Aquifex aeolicus and Escherichia coli leucyl-tRNA synthetases. Biochemistry. 42:2003;7694-7700
8. Args. Biotechniques. 19:1995;196-200
9. Arg(UCU) induces a constrained conformation of the crucial Ω-loop of arginyl-tRNA synthetase. Biochem. Biophys. Res. Commun. 313:1995;129-134
10. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J. Mol. Biol. 212:1990;579-598
11. Garcia G.M., Mar P.K., Mullin D.A., Walker J.R., Prather N.E. The E. coli dnaY gene encodes an arginine transfer RNA. Cell. 45:1986;453-459
12. Dieci G., Bottarelli L., Ballabeni A., Ottonello S. TRNA-assisted overproduction of eukaryotic ribosomal proteins. Protein Expr. Purif. 18:2000;346-354
13. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
14. Li T., Guo N.N., Xia X., Wang E.D., Wang Y.L. The peptide bond between E292-A293 of Escherichia coli leucyl-tRNA synthetase is essential for its activity. Biochemistry. 38:1999;13063-13069