Insights into Vitamin D metabolism using cyp24 over-expression and knockout systems in conjunction with liquid chromatography/mass spectrometry (LC/MS)

Journal of Steroid Biochemistry and Molecular Biology

Volumn 89-90, Issue , 2004, Pages 149-153

  Masuda, Sonoko ^a   Kaufmann, Martin ^a   Byford, Valarie ^a   Gao, Mian ^b   St Arnaud, René ^c   Arabian, Alice ^c   Makin, Hugh L J ^d   Knutson, Joyce C ^e   Strugnell, Stephen ^e   Jones, Glenville ^a  

^a QUEEN S UNIVERSITY   (Canada)

^b Cytochroma Inc   (Canada)

^c Genetics Unit   (Canada)

^d QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^e Bone Care International   (United States)

Author keywords

1 Hydroxyvitamin D2; 24 Hydroxylation; CYP24; CYP24 knockout mouse; Cytochrome P450; DBP; LC MS; Vitamin D catabolism

Indexed keywords

1ALPHA,24,25 DIHYDROXYVITAMIN D3; CALCIFEDIOL; CALCITRIOL; COLECALCIFEROL; CYTOCHROME P450; UNCLASSIFIED DRUG; VITAMIN D;

ANALYTIC METHOD; ANIMAL CELL; CONCENTRATION RESPONSE; CONFERENCE PAPER; CONTROLLED STUDY; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; IN VIVO STUDY; KERATINOCYTE; KNOCKOUT GENE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOUSE; NONHUMAN; VITAMIN METABOLISM; WILD TYPE;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 ENZYME SYSTEM; MASS SPECTROMETRY; MICE; MICE, KNOCKOUT; STEROID HYDROXYLASES; VITAMIN D;

ANIMALIA; MURINAE;

EID: 3042543862     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2004.03.094     Document Type: Conference Paper

References (10)

1. Cytochrome P450, Homepage at http://drnelson.utmem.edu/humP450.aln.html.
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5. 3, the preferred substrate for CYP24. J. Bone Miner. Res. 17:2001;179-181
6. 2 made in the human hepatoma, Hep3B. Biochem. J. 310:1995;233-241
7. 3 to calcitroic acid: Evidence for a pathway in kidney and bone involving 24-oxidation. Biochem. J. 262:1989;173-180
8. 3 in blood and tissues of the CYP24-null mouse, J. Bone Miner. Res. 15 (2000) S199.
9. 3. J. Bone Miner. Res. 16:2001;57-62
10. 2 to calcitroic acid. J. Cell Biochem. 88:2003;282-285