Apple polygalacturonase inhibiting protein1 expressed in transgenic tobacco inhibits polygalacturonases from fungal pathogens of apple and the anthracnose pathogen of lupins

Phytochemistry

Volumn 67, Issue 3, 2006, Pages 255-263

  Oelofse, Dean ^a,b,c   Dubery, Ian A ^b   Meyer, Riaan ^b   Arendse, Melanie S ^a   Gazendam, Inge ^a   Berger, Dave K ^c  

^a AGRICULTURAL RESEARCH COUNCIL   (South Africa)

^b UNIVERSITY OF JOHANNESBURG   (South Africa)

^c UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Apple; Aspergillus niger; Botryosphaeria obtusa; Colletotrichum lupini; Diaporthe ambigua; Malus domestica; Nicotiana tabacum; PGIP; Polygalacturonase

Indexed keywords

ANTIFUNGAL AGENT; ENZYME INHIBITOR; PGIP PROTEIN, PLANT; POLYGALACTURONASE; VEGETABLE PROTEIN;

APPLE; ARTICLE; ASCOMYCETES; ASPERGILLUS NIGER; COLLETOTRICHUM; DRUG ANTAGONISM; ENZYMOLOGY; FUNGUS; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PATHOGENICITY; TOBACCO; TRANSGENIC PLANT;

ANTIFUNGAL AGENTS; ASCOMYCOTA; ASPERGILLUS NIGER; COLLETOTRICHUM; ENZYME INHIBITORS; FUNGI; MALUS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYGALACTURONASE; TOBACCO; TRANSFORMATION, GENETIC;

AGROBACTERIUM; ASPERGILLUS NIGER; BOTRYOSPHAERIA OBTUSA; COLLETOTRICHUM; COLLETOTRICHUM LUPINI; DIAPORTHE AMBIGUA; MALUS X DOMESTICA; NICOTIANA TABACUM; RHIZOBIUM;

EID: 30344441796     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2005.10.029     Document Type: Article

References (35)

1. P. Albersheim, and A.J. Anderson Proteins from plant cell walls inhibit polygalacturonases secreted by plant pathogens Proc. Natl. Acad. Sci. USA 68 1971 1815 1819
2. M.S. Arendse, I.A. Dubery, and D.K. Berger Isolation by PCR-based methods of a plant antifungal polygalacturonase-inhibiting protein gene Electron. J. Biotech. 2 1999 152 159
3. D.K. Berger, D. Oelofse, M.S. Arendse, E. Du Plessis, and I.A. Dubery Bean polygalacturonase inhibiting protein-1 (PGIP-1) inhibits polygalacturonases from Stenocarpella maydis Physiol. Mol. Plant P. 57 2000 5 14
4. H. Blum, H. Beier, and H.G. Gross Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels Electrophoresis 8 1987 93 99
5. M.M. Bradford A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principal of protein-dye binding Anal. Biochem. 72 1976 248 254
6. A.E. Brown Relationship of endopolygalacturonase inhibitor activity to the fungal rot development in apple fruits Phytopathol. Z. 111 1984 122 132
7. B.J. Cook, R.P. Clay, C.W. Bergmann, P. Albersheim, and A.G. Darvill Fungal polygalacturonases exhibit different substrate degradation patterns and differ in their susceptibilities to polygalacturonase-inhibiting proteins Mol. Plant Microbe In. 12 1999 703 711
8. M.M.R. Costa, J. Costa, and C.C.P. Ricardo A Lupinus albus root glycoprotein homologous to the polygalacturonase inhibitor proteins Physiol. Plantarum 99 1997 263 270
9. G. De Lorenzo, R. D'Ovidio, and F. Cervone The role of polygalacturonase-inhibiting proteins (PGIPs) in defense against pathogenic fungi Annu. Rev. Phytopathol. 39 2001 313 335
10. A. Desiderio, B. Aracri, F. Leckie, B. Mattei, and G. Salvi Polygalacturonase-inhibiting proteins (PGIPs) with different specificities are expressed in Phaseolus vulgaris Mol. Plant Microbe In. 10 1997 852 860
11. P.D. English, J.B. Jurale, and P. Albersheim Host-pathogen interactions II: Parameters affecting polysaccharide-degrading enzyme secretion by Colletotrichum lindemuthianum grown in culture Plant Physiol. 47 1971 1 6
12. F. Favaron Gel detection of Allium porrum polygalacturonase-inhibiting protein reveals a high number of isoforms Physiol. Mol. Plant Pathol. 58 2005 239 245
13. S. Ferrari, D. Vairo, F.M. Ausubel, F. Cervone, and G. De Lorenzo Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection Plant Cell 15 2003 93 106
14. M. Frediani, R. Cremonini, G. Salvi, C. Caprari, A. Desiderio, R. D'Ovidio, F. Cervone, and G. De Lorenzo Cytological localization of the pgip genes in embryo suspensor cells of Phaseolus vulgaris L Theor. Appl. Genet. 87 1993 369 373
15. W.S. Grayburn, and B.A. Vick Transformation of sunflower (Helianthus anuus L.) following wounding with glass beads Plant Cell Rep. 14 1995 285 289
16. R.B. Horsch, J.E. Fry, N. Hoffman, J. Neidermeyer, S.G. Rogers, and R.T. Fraley Leaf disc transformation S.B. Gelvin R.A. Schilperoort D.P.S. Verna Plant Molecular Biology Manual, A5 1992 Kluwer Academic Publishers Dordrecht 1 9
17. A.L. Karr, and P. Albersheim Polysaccharide-degrading enzymes are unable to attack plant cell walls without prior action by a "wall-modifying enzyme" Plant Physiol. 46 1970 69 80
18. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of Bacteriophage T4 Nature 227 1970 680 685
19. F. Leckie, B. Mattei, C. Capodicasa, A. Hemmings, L. Nuss, B. Acrari, G. De Lorenzo, and F. Cervone The specificity of polygalacturonase-inhibiting protein (PGIP): a single amino acid substitution in the solvent-exposed β-strand/β-turn region of the leucine-rich repeats (LRRs) confers new recognition capability Embo J. 18 1999 2352 2363
20. H.C. Lotter, and D.K. Berger Anthracnose of lupins in South Africa is caused by Colletotrichum lupini var. setosum Australasian Plant Pathol. 34 2005 385 392
21. L. Mehli, J.G. Schaart, T.D. Kjellsen, D. Hong Tran, E.M.J. Salentijn, H.J. Schouten, and T.-H. Iversen A gene encoding a polygalacturonase-inhibiting protein (PGIP) shows developmental regulation and pathogen-induced expression in strawberry New Phytologist 163 2004 99 110
22. M. Müller, and C. Gessler A protein from apple leaves inhibits pectinolytic activity of Venturia inequalis in vitro B. Fritig M. Legrand Mechanisms of Plant Defense Responses 1993 Kluwer Academic Publishers Dordrecht 68 71
23. M.G. Murray, and W.F. Thompson Rapid isolation of high molecular weight plant DNA Nucleic Acids Res. 8 1980 4321 4325
24. A.L.T. Powell, J. van Kan, A. ten Have, J. Visser, L.C. Greve, A.B. Bennett, and J.M. Labavitch Transgenic expression of pear PGIP in tomato limits fungal colonisation Mol. Plant Microbe In. 13 2000 942 950
25. M.A. Restrepo, D.D. Freed, and J.C. Carrington Nuclear transport of plant potyviral proteins Plant Cell 2 1990 987 998
26. E. Robertson, H.K. Dannelly, P.J. Malloy, and H.C. Reeves Rapid isoelectric focusing in a vertical polyacrylamide minigel system Anal. Biochem. 167 1987 290 294
27. G. Salvi, F. Giarrizzo, G. De Lorenzo, and F. Cervone A polygalacturonase-inhibiting protein in the flowers of Phaseolus vulgaris L Plant Physiol. 136 1990 513 518
28. W.A. Smit, B.D. Wingfield, and M.J. Wingfield Vegetative incompatibilty in Diaporthe ambigua Plant Pathol. 46 1997 366 372
29. H.U. Stotz, A.L.T. Powell, S.E. Damon, L.C. Greve, A.B. Bennett, and J.M. Labavitch Molecular characterization of a polygalacturonase inhibitor from Pyrus communis L cv Bartlett Plant Physiol. 102 1993 133 138
30. H.U. Stotz, J.J.A. Contos, A.L.T. Powell, A.B. Bennett, and J.M. Labavitch Structure and expression of an inhibitor of fungal polygalacturonases from tomato Plant Mol. Biol. 25 1994 607 617
31. H.U. Stotz, J.G. Bishop, C.W. Bergmann, M. Koch, and P. Albersheim Identification of target amino acids that affect interactions of fungal polygalacturonases and their plant inhibitors Physiol. Mol. Plant P. 56 2000 117 2130
32. R.J. Taylor, and G.A. Secor An improved diffusion assay for quantifying the polygalacturonase content of Erwinia culture filtrates Phytopathology 78 1988 1101 1103
33. J.P. Wubben, W. Mulder, A. ten Have, J.A.L. van Kan, and J. Visser Cloning and partial characterisation of endopolygalacturonase genes from Botrytis cinerea Appl. Environ. Microb. 65 1999 1596 1602
34. C.L. Yao, W.S. Conway, and C.E. Sams Purification and characterisation of a polygalacturonase-inhibiting protein from apple fruit Phytopathology 85 1995 1373 1377
35. C.L. Yao, W.S. Conway, R. Ren, D. Smith, G.S. Ross, and C.E. Sams Gene encoding polygalacturonase inhibitor in apple fruit is developmentally regulated and activated by wounding and fungal infection Plant Mol. Biol. 39 1999 1231 1241