Common methodology is inadequate for studies on the microbicidal activity of neutrophils

Journal of Leukocyte Biology

Volumn 79, Issue 1, 2006, Pages 87-94

  Decleva, Eva ^a   Menegazzi, Renzo ^a   Busetto, Sara ^a   Patriarca, Pierluigi ^a   Dri, Pietro ^a  

^a UNIVERSITY OF TRIESTE   (Italy)

Author keywords

CGD; Diphenyleneiodonium; Microbial killing; MPO deficiency

Indexed keywords

DIPHENYLIODONIUM SALT; MYELOPEROXIDASE; PHORBOL 13 ACETATE 12 MYRISTATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; SUPEROXIDE; WATER;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL COUNT; BACTERICIDAL ACTIVITY; CANDIDA ALBICANS; CHRONIC GRANULOMATOUS DISEASE; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOLYSIS; ENZYME DEFICIENCY; ESCHERICHIA COLI; FLOW CYTOMETRY; FUNGICIDAL ACTIVITY; HUMAN; HUMAN CELL; MICROSCOPY; NEUTROPHIL; PH; PRIORITY JOURNAL; REPRODUCIBILITY; STAPHYLOCOCCUS AUREUS;

BIOLOGICAL ASSAY; CANDIDA ALBICANS; CELLS, CULTURED; COLONY COUNT, MICROBIAL; ENZYME INHIBITORS; ESCHERICHIA COLI; GRANULOMATOUS DISEASE, CHRONIC; HUMANS; HYDROGEN-ION CONCENTRATION; MICROBIAL VIABILITY; NEUTROPHILS; ONIUM COMPOUNDS; PEROXIDASE; PHAGOCYTOSIS; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); CANDIDA ALBICANS; ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

EID: 30144445925     PISSN: 07415400     EISSN: None     Source Type: Journal    
DOI: 10.1189/jlb.0605338     Document Type: Article

References (35)

1. Johnston, R. B., Newman, S. L. (1977) Chronic granulomatous disease. Pediatr. Clin. North Am. 24, 365-376.
2. Segal, B. H., Leto, T. L., Gallin, J. I., Malech, H. L., Holland, S. M. (2000) Genetic, biochemical, and clinical features of chronic granulomatous disease. Medicine (Baltimore) 79, 170-200.
3. Quie, P. G., White, J. G., Holmes, B., Good, R. A. (1967) In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood. J. Clin. Invest. 46, 668-679.
4. Repine, J. E., Clawson, C. C. (1977) Quantitative measurement of the bactericidal capability of neutrophils from patients and carriers of chronic granulomatous disease. J. Lab. Clin. Med. 90, 522-528.
5. Miyazaki, S., Shin, H., Goya, N., Nakagawara, A. (1976) Identification of a carrier mother of a female patient with chronic granulomatous disease. J. Pediatr. 89, 784-786.
6. Clark, F. A., Klebanoff, S. J. (1978) Chronic granulomatous disease. Studies of a family with impaired neutrophil chemotactic, metabolic and bactericidal function. Am. J. Med. 65, 941-948.
7. Segal, A. W., Geisow, M., Garcia, R., Harper, A., Miller, R. (1981) The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH. Nature 290, 406-409.
8. Segal, A. W., Harper, A. M., Garcia, R. C., Merzbach, D. (1982) The action of cells from patients with chronic granulomatous disease on Staphylococcus aureus. J. Med. Microbiol. 15, 441-449.
9. D'Amelio, R., Bellavite, P., Bianco, P., de Sole, P., Le Moli, S., Lippa, S., Seminara, R., Vercelli, B., Rossi, F., Rocchi, G., Aiuti, F. (1984) Chronic granulomatous disease in two sisters. J. Clin. Immunol. 4, 220-227.
10. Ellis, J. A., Mayer, S. J., Jones, O. T. G. (1988) The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils. Biochem. J. 251, 887-891.
11. Speert, D. P., Bond, M., Woodman, R. C., Curnutte, J. T. (1994) Infection with Pseudomonas cepacia in chronic granulomatous disease: role of nonoxidative killing by neutrophils in host defense. J. Infect. Dis. 170, 1524-1531.
12. Hampton, M. B., Winterbourn, C. C. (1995) Modification of neutrophil oxidant production with diphenyleneiodonium and its effect on bacterial killing. Free Radic. Biol. Med. 18, 633-639.
13. - variant chronic granulomatous disease. Blood 86, 3575-3582.
14. Kowanko, I. C., Ferrante, A., Clemente, G., Kumaratilake, L. M. (1996) Tumor necrosis factor primes neutrophils to kill Staphylococcus aureus by an oxygen-dependent mechanism and Plasmodium falciparum by an oxygen-independent mechanism. Infect. Immun. 64, 3435-3437.
15. Malawista, S. E., Montgomery, R. R., Van Blaricom, G. (1996) Microbial killing by human neutrophil cytokineplasts: similar suppressive effects of reversible and irreversible inhibitors of nitric oxide synthase. J. Leukoc. Biol. 60, 753-757.
16. Gerber, C., Bruchelt, G., Falk, U. B., Kimpfler, A., Hauschild, O., Kuçi, S., Bächi, T., Niethammer, D., Schubert, R. (2001) Reconstitution of bactericidal activity in chronic granulomatous disease cells by glucose-oxidase-containing liposomes. Blood 98, 3097-3105.
17. Yamashita, K., Arai, T., Fukuda, K., Mori, H., Ishii, H., Nishioka, M., Tajima, K., Makino, K., Sasada, M. (2001) 6-Formylpterin intracellularly generates hydrogen peroxide and restores the impaired bactericidal activity of human neutrophils. Biochem. Biophys. Res. Commun. 289, 85-90.
18. + flux. Nature 416, 291-297.
19. Cross, A. R., Jones, O. T. (1986) The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labeling of a component polypeptide of the oxidase. Biochem. J. 237, 111-116.
20. Metcalf, J. A., Gallin, J. I., Nauseef, W. M., Root, R. K. (1986) Microbicidal assay. In Laboratory Manual of Neutrophil Function, New York, NY, Raven, 134-143.
21. Babior, B. M., Cohen, H. J. (1981) Measurement of neutrophil function; phagocytosis, degranulation, the respiratory burst and bacterial killing. In Methods in Haematology, vol. 3 (M. J. Cline, ed.), New York, NY, Churchill-Livingstone, 1-14.
22. Leijh, P. C. J., Van Furth, R., Van Zwet, T. L. (1986) In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes. In Handbook of Experimental Immunology, vol. 2 (D. M. Weir, ed.), Oxford, UK, Blackwell Scientific Publication Ltd., 46.1-46.21.
23. Gargan, R. A., Brumfitt, W., Hamilton-Miller, J. M. T. (1989) Failure of water to lyse polymorphonuclear neutrophils completely. Role of pH and implications for assessment of bacterial killing. J. Immunol. Methods 124, 289-291.
24. Dri, P., Presani, G., Perticarari, S., Albèri, L., Prodan, M., Decleva, E. (2002) Measurement of phagosomal pH in human neutrophils by dual fluorescence flow cytometry. Cytometry 48, 159-166.
25. Menegazzi, R., Cramer, R., Patriarca, P., Scheurich, P., Dri, P. (1994) Evidence that tumor necrosis factor-induced activation of neutrophil respiratory burst on biological surfaces is mediated by the p55 TNF receptor. Blood 84, 287-293.
26. Weiss, J., Kao, L., Victor, M., Elsbach, P. (1985) Oxygen-independent intracellular and oxygen-dependent extracellular killing of Escherichia coli S15 by human polymorphonuclear leukocytes. J. Clin. Invest. 76, 206-212.
27. Rosen, H., Michel, B. R. (1997) Redundant contribution of myeloperoxidase-dependent systems to neutrophil-mediated killing of Escherichia coli. Infect. Immun. 65, 4173-4178.
28. Lehrer, R. I., Cline, M. J. (1969) Leukocyte myeloperoxidase deficiency and disseminated candidiasis: the role of myeloperoxidase in resistance to Candida infection. J. Clin. Invest. 48, 1478-1488.
29. Parry, M. F., Root, R. K., Metcalf, J. A., Delaney, K. K., Kaplow, L. S., Richar, W. J. (1981) Myeloperoxidase deficiency: prevalence and clinical significance. Ann. Intern. Med. 95, 293-301.
30. Stendahl, O., Lindgren, S. (1976) Function of granulocytes with deficient myeloperoxidase-mediated iodination in a patient with generalized pustular psoriasis. Scand. J. Haematol. 16, 144-153.
31. Robertson, C. F., Thong, Y. H., Hodge, G. L., Cheney, K. (1979) Primary myeloperoxidase deficiency associated with impaired neutrophil margination and chemotaxis. Acta Paediatr. Scand. 68, 915-919.
32. Kitahara, M., Eyre, H. J., Simonian, Y., Atkin, C. L., Hasstedt, S. J. (1981) Hereditary myeloperoxidase deficiency. Blood 57, 888-893.
33. Larrocha, C., Fernández de Castro, M., Fontan, G., Viloria, A., Fernández-Chacón, J. L., Jiménez, C. (1982) Hereditary myeloperoxidase deficiency: study of 12 cases. Scand. J. Haematol. 29, 389-397.
34. Rada, B. K., Geiszt, M., Káldi, K., Timár, C., Ligeti, E. (2004) Dual role of phagocytic NADPH oxidase in bacterial killing. Blood 104, 2947-2952.
35. Dri, P., Cramer, R., Menegazzi, R., Patriarca, P. (1987) Biochemical, functional and clinical aspects of hereditary human myeloperoxidase deficiency. In Advances in Biosciences, vol. 66 (C. Mauri, S. C. Rizzo, G. Ricevuti, eds.), Oxford, UK, Pergamon, 193-206.