Constitutive expression of catABC genes in the aniline-assimilating bacterium Rhodococcus species AN-22: Production, purification, characterization and gene analysis of CatA, CatB and CatC

Biochemical Journal

Volumn 393, Issue 1, 2006, Pages 219-226

  Matsumura, Eitaro ^a   Sakai, Masashi ^a   Hayashi, Katsuaki ^a   Murakami, Shuichiro ^a   Takenaka, Shinji ^a   Aoki, Kenji ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Aniline assimilating bacterium?; cis,cis muconate cycloisomerase; Constitutive expression; Muconolactone isomerase; Primer extension analysis; Rhodococcus

Indexed keywords

AMINES; AMINO ACIDS; ENZYMES; GENES; MICROORGANISMS; MONOMERS;

ANILINE-ASSIMILATING BACTERIUM?; CIS,CIS-MUCONATE CYCLOISOMERASE; CONSTITUTIVE EXPRESSION; MUCONOLACTONE ISOMERASE; PRIMER EXTENSION ANALYSIS; RHODOCOCCUS;

BACTERIA;

ANILINE; BACTERIAL DNA; CATECHOL 1,2 DIOXYGENASE; COBALT; ISOMERASE; MAGNESIUM ION; MANGANESE; MESSENGER RNA; MUCONIC ACID CYCLOISOMERASE; MUCONOLACTONE DELTA ISOMERASE; REGULATOR PROTEIN; SUCCINIC ACID; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CATA GENE; CATABC GENE; CATABOLISM; CATB GENE; CATC GENE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; GENE CLUSTER; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHODOCOCCUS; RNA TRANSLATION; START CODON; TRANSCRIPTION INITIATION SITE;

ANILINE COMPOUNDS; BACTERIAL PROTEINS; CATECHOL 1,2-DIOXYGENASE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; ISOMERASES; MOLECULAR SEQUENCE DATA; RHODOCOCCUS; SUBSTRATE SPECIFICITY; TRANSCRIPTION INITIATION SITE;

BACTERIA (MICROORGANISMS); POSIBACTERIA; RHODOCOCCUS; RHODOCOCCUS SP. AN-22;

EID: 30044434149     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050740     Document Type: Article

References (40)

1. Lyons, C. D., Katz, S. E. and Bartha, R. (1984) Mechanisms and pathways of aniline elimination from aquatic environments. Appl. Environ. Microbiol. 48, 491-496
2. Aoki, K., Shinke, R. and Nishira, H. (1982) Identification of aniline-assimilating bacteria. Agric. Biol. Chem. 46, 2563-2570
3. Aoki, K., Shinke, R. and Nishira, H. (1983) Metabolism of aniline by Rhodococcus erythropolis AN-13. Agric. Biol. Chem. 48, 1611-1616
4. Aoki, K., Konohana, T., Shinke, R. and Nishira, H. (1984) Purification and characterization of catechol 1,2-dioxygenase from aniline-assimilating Rhodococcus erythropolis AN-13. Agric. Biol. Chem. 48, 2087-2095
5. Aoki, K., Konohana, T., Shinke, R. and Nishira, H. (1984) Two catechol 1,2-dioxygenases from an aniline-assimilating bacterium, Frateuria sp. ANA-18. Agric. Biol. Chem. 48, 2097-2104
6. + aniline-assimilating Rhodococcus erythropolis AN-13. Gene 185, 49-54
7. Murakami, S., Hayashi, T., Maeda, T., Takenaka, S. and Aoki, K. (2003) Cloning and functional analysis of aniline dioxygenase gene cluster, from Frateuria species ANA-18, that metabolizes aniline via an ortho-cleavage pathway of catechol. Biosci. Biotechnol. Biochem. 67, 2351-2358
8. Murakami, S., Takashima, A., Takemoto, J., Takenaka, S., Shinke, R. and Aoki, K. (1999) Cloning and sequence analysis of two catechol-degrading gene clusters from the aniline-assimilating bacterium Frateuria species ANA-18. Gene 226, 189-198
9. Matsumura, E., Ooi, S., Murakami, S., Takenaka, S. and Aoki, K. (2004) Constitutive synthesis, purification, and characterization of catechol 1,2-dioxygenase from the aniline-assimilating bacterium Rhodococcus sp. AN-22. J. Biosci. Bioeng. 98, 71-76
10. Takashima, A., Murakami, S., Takenaka, S. and Aoki, K. (2001) Regulation by two CatR proteins that differ in binding affinity to catB promoters expressing two cat gene clusters. Biosci. Biotechnol. Biochem. 65, 2146-2153
11. Lechner, U. and Straube, G. (1988) Degradation of 3-aminophenol by Arthrobacter spec. mA3. J. Basic Microbiol. 28, 629-637
12. Roldan, M. D., Blasco, R., Caballero, F. J. and Castillo, F. (1998) Degradation of p-nitrophenol by the phototrophic bacterium Rhodobacter capsulatus. Arch. Microbiol. 169, 36-42
13. Caposio, P., Pessione, E., Giuffrida, G., Conti, A., Landolfo, S, Giunta, C. and Gribaudo, G. (2002) Cloning and characterization of two catechol 1,2-dioxygenase genes from Acinetobacter radioresistens S13. Res. Microbiol. 153, 69-74
14. Aoki, K., Ohtsuka, K., Shinke, R. and Nishira, H. (1983) Isolation of aniline-assimilating bacteria and physiological characterization of aniline biodegradation in Rhodococcus erythropolis AN-13. Agric. Biol. Chem. 47, 2569-2575
15. Liu, S., Ogawa, N. and Miyasnita, K. (2001) The chlorocatechol degradative genes, tfdT-CDEF, of Burkholderia sp. strain NK8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols. Gene 268, 207-214
16. Murakami, S., Takemoto, J., Takashima, A., Shinke, R. and Aoki, K. (1998) Purification and characterization of two muconate cycloisomerase isozymes from aniline-assimilating Frateuria species ANA-18. Biosci. Biotechnol. Biochem. 62, 1129-1133
17. Ornston, L. N. (1966) The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. III. Enzymes of the catechol pathway. J. Biol. Chem. 241, 3795-3799
18. Moiseeva, O. V., Solyanikova, I. P., Kaschabek, S. R., Groning, J., Thiel, M., Golovleva, L. A. and Schlömann, M. (2002) A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence. J. Bacteriol. 184, 5282-5292
19. Ornston, L. N. (1966) The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. II. Enzymes of the protocatechuate pathway. J. Biol. Chem. 241, 3787-3794
20. 2 oligomeric structures in β-ketoadipate succinyl-CoA transferases from Acinetobacter calcoaceticus and Pseudomonas putida. J. Biol. Chem. 256, 1565-1569
21. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol regent. J. Biol. Chem. 193, 265-275
22. Weber, K. and Osborn, M. (1969) The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406-4412
23. Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262, 10035-10038
24. Murakami, S., Kohsaka, C., Okuno, T., Takenaka, S. and Aoki, K. (2004) Purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17. FEMS Microbiol. Lett. 231, 119-124
25. Dorn, E. and Knackmuss, H.-J. (1978) Chemical structure and biodegradability of halogenated aromatic compounds: substituent effects on 1,2-dioxygenation of catechol. Biochem. J. 174, 85-94
26. Sambrook, J., Frisch, E. F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor
27. DiLella, A. G. and Woo, S. L. C. (1987) Cloning large segments of genomic DNA using cosmid vectors. Methods Enzymol. 152, 199-212
28. Murakami, S., Sawami, Y., Takenaka, S. and Aoki, K. (2004) Cloning of a gene encoding 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. 10d. Biochem. Biophys. Res. Commun. 314, 489-494
29. Strachan, P. D., Freer, A. A. and Fewson, C. A. (1998) Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene. Biochem. J. 333, 741-747
30. Eulberg, D., Golovleva, L. A. and Schlömann, M. (1997) Characterization of catechol catabolic genes from Rhodococcus erythropolis 1CP. J. Bacteriol. 179, 370-381
31. Park, H.-J. and Kim, E.-S. (2003) An inducible Streptomyces gene cluster involved in aromatic compound metabolism. FEMS Microbiol. Lett. 226, 151-157
32. Eulberg, D. and Schlomann, M. (1998) The putative regulator of catechol catabolism in Rhodococcus opacus 1CP: an IcIR-type, not a LysR-type transcriptional regulator. Antonie Van Leeuwenhoek 74, 71-82
33. Yao, W., Yang, Y. and Chiao, J. (1994) IS204: an insertion sequence from Nocardia asteroides (mexicana) YP21. Plasmid 32, 262-269
34. Murakami, S., Takemoto, J., Takenaka, S., Shinke, R. and Aoki, K. (1998) Purification and characterization of muconate cycloisomerase from aniline-assimilating Rhodococcus erythropolis AN-13. J. Ferment. Bioeng. 85, 521-524
35. Vollmer, M. D., Hoier, H., Hecht, H. J., Schell, U., Gröning, J., Goldman, A. and Schlömann, M. (1998) Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants. Appl. Environ. Microbiol. 64, 3290-3299
36. Solyanikova, I. P., Maltseva, O. V., Vollmer, M. D., Golovleva, L. A. and Schlömann, M. (1995) Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP: indications for functionally convergent evolution among bacterial cycloisomerases. J. Bacteriol. 177, 2821-2826
37. Houghton, J. E., Brown, T. M., Appel, A. J., Hughes, E. J. and Ornston, L. N. (1995) Discontinuities in the evolution of Pseudomonas putida cat genes. J. Bacteriol. 177, 401-412
38. Iida, T., Mukouzaka, Y., Nakamura, K., Yamaguchi, I. and Kudo, T. (2002) Isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading Rhodococcus species. Biosci. Biotechnol. Biochem. 66, 1462-1472
39. Bell, K. S., Philp, J. C., Aw, D. W. J. and Christofi, N. (1998) The genus Rhodococcus. J. Appl. Microbiol. 85, 195-210
40. O'Brien, X. M., Parker, J. A., Lessard, P. A. and Sinskey, A. J. (2002) Engineering an indene bioconversion process for the production of cis-aminoindanol: a model system for the production of chiral synthons. Appl. Microbiol. Biotechnol. 59, 389-399