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Volumn 32, Issue 1, 2006, Pages 65-71

Megaprimer-based methodology for deletion of a large fragment within a repetitive polypyrimidine-rich DNA

Author keywords

Large deletion; Megaprimer; Repetitive sequence

Indexed keywords

BINDING ENERGY; CROSSLINKING; ENZYMES; GENETIC ENGINEERING; MUTAGENESIS; NITROGEN COMPOUNDS; RNA;

EID: 29644445307     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:32:1:065     Document Type: Article
Times cited : (10)

References (21)
  • 1
    • 0034813301 scopus 로고    scopus 로고
    • Multifunctional regulatory proteins that control gene expression in both the nucleus and the cytoplasm
    • Wilkinson, M. F. and Shyu, A.B. (2001) Multifunctional regulatory proteins that control gene expression in both the nucleus and the cytoplasm. Bioessays 23, 775-787.
    • (2001) Bioessays , vol.23 , pp. 775-787
    • Wilkinson, M.F.1    Shyu, A.B.2
  • 2
    • 0036070957 scopus 로고    scopus 로고
    • Inflammation modulates the interaction of heterogeneous nuclear ribonucleoprotein (hnRNP) I/polypyrimidine tract binding protein and hnRNP L with the 3′ untranslated region of the murine inducible nitric-oxide synthase mRNA
    • Söderberg, M., Raffalli-Mathieu, F., and Lang, M. A. (2002) Inflammation modulates the interaction of heterogeneous nuclear ribonucleoprotein (hnRNP) I/polypyrimidine tract binding protein and hnRNP L with the 3′ untranslated region of the murine inducible nitric-oxide synthase mRNA. Mol. Pharmacol. 62, 423-431.
    • (2002) Mol. Pharmacol. , vol.62 , pp. 423-431
    • Söderberg, M.1    Raffalli-Mathieu, F.2    Lang, M.A.3
  • 3
    • 0037472649 scopus 로고    scopus 로고
    • Interplay between hnRNP A1 and a cis-acting element in the 3′ UTR of CYP2A5 mRNA is central for high expression of the gene
    • Glisovic, T., Ben-David, Y., Lang, M. A., and Raffalli-Mathieu, F. (2003) Interplay between hnRNP A1 and a cis-acting element in the 3′ UTR of CYP2A5 mRNA is central for high expression of the gene. FEBS Lett. 535, 147-152.
    • (2003) FEBS Lett. , vol.535 , pp. 147-152
    • Glisovic, T.1    Ben-David, Y.2    Lang, M.A.3    Raffalli-Mathieu, F.4
  • 4
    • 2442582602 scopus 로고    scopus 로고
    • Post-transcriptional regulation of glucose transporter-1 by an AU-rich element in the 3′ UTR and by hnRNP A2
    • Griffin, M. E., Hamilton, B. J., Roy, K. M., et al. (2004) Post-transcriptional regulation of glucose transporter-1 by an AU-rich element in the 3′ UTR and by hnRNP A2. Biochem. Biophys. Res. Commun. 318, 977-982.
    • (2004) Biochem. Biophys. Res. Commun. , vol.318 , pp. 977-982
    • Griffin, M.E.1    Hamilton, B.J.2    Roy, K.M.3
  • 5
    • 0031574019 scopus 로고    scopus 로고
    • Approaches to DNA mutagenesis: An overview
    • Ling, M. M. and Robinson, B. H. (1997) Approaches to DNA mutagenesis: an overview. Anal Biochem. 254, 157-178.
    • (1997) Anal Biochem. , vol.254 , pp. 157-178
    • Ling, M.M.1    Robinson, B.H.2
  • 7
    • 0024346633 scopus 로고
    • Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)
    • Kammann, M., Laufs, J., Schell, J., and Gronenborn, B. (1989) Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acids Res. 17, 5404.
    • (1989) Nucleic Acids Res. , vol.17 , pp. 5404
    • Kammann, M.1    Laufs, J.2    Schell, J.3    Gronenborn, B.4
  • 8
    • 1542285933 scopus 로고    scopus 로고
    • Generation of large deletion mutants from plasmid DNA
    • Stoynova, L., Solorzano, R., and Collins, E. D. (2004) Generation of large deletion mutants from plasmid DNA. Biotechniques 36, 402-406.
    • (2004) Biotechniques , vol.36 , pp. 402-406
    • Stoynova, L.1    Solorzano, R.2    Collins, E.D.3
  • 9
    • 0031898207 scopus 로고    scopus 로고
    • Fusion PCR, a one-step variant of the "megaprimer" method of mutagenesis
    • Karreman, C. (1998) Fusion PCR, a one-step variant of the "megaprimer" method of mutagenesis. Biotechniques 24, 736-742.
    • (1998) Biotechniques , vol.24 , pp. 736-742
    • Karreman, C.1
  • 10
    • 0037098946 scopus 로고    scopus 로고
    • Site-directed mutagenesis by the megaprimer PCR method: Variations on a theme for simultaneous introduction of multiple mutations
    • Angelaccio, S. and Bonaccorsi di Patti, M. C. (2002) Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations. Anal Biochem. 306, 346-349.
    • (2002) Anal Biochem. , vol.306 , pp. 346-349
    • Angelaccio, S.1    Bonaccorsi Di Patti, M.C.2
  • 11
    • 0032968931 scopus 로고    scopus 로고
    • Inverse PCR-generated internally deleted constructs for direct characterization of promoter regulatory regions
    • Kaluz, S., Kaluzova, M., and Pastorek, J. (1999) Inverse PCR-generated internally deleted constructs for direct characterization of promoter regulatory regions. Biotechniques 26, 446-450.
    • (1999) Biotechniques , vol.26 , pp. 446-450
    • Kaluz, S.1    Kaluzova, M.2    Pastorek, J.3
  • 12
    • 0025325983 scopus 로고
    • The "megaprimer" method of site-directed mutagenesis
    • Sarkar, G. and Sommer, S. S. (1990) The "megaprimer" method of site-directed mutagenesis. Biotechniques 8, 404-407.
    • (1990) Biotechniques , vol.8 , pp. 404-407
    • Sarkar, G.1    Sommer, S.S.2
  • 13
    • 0029682364 scopus 로고    scopus 로고
    • A simple method to introduce internal deletions or mutations into any position of a target DNA sequence
    • Tomic-Canic, M., Bernerd, F., and Blumenberg, M. (1996) A simple method to introduce internal deletions or mutations into any position of a target DNA sequence. Methods Mol. Biol. 57, 249-257.
    • (1996) Methods Mol. Biol. , vol.57 , pp. 249-257
    • Tomic-Canic, M.1    Bernerd, F.2    Blumenberg, M.3
  • 14
    • 0029684151 scopus 로고    scopus 로고
    • PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments
    • Tessier, D. C. and Thomas, D. Y. (1996) PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments. Methods Mol. Biol. 57, 229-237.
    • (1996) Methods Mol. Biol. , vol.57 , pp. 229-237
    • Tessier, D.C.1    Thomas, D.Y.2
  • 15
    • 0024357799 scopus 로고
    • A simple method for site-directed mutagenesis using the polymerase chain reaction
    • Hemsley, A., Arnheim, N., Toney, M. D., Cortopassi, G., and Galas, D. J. (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 17, 6545-6551.
    • (1989) Nucleic Acids Res. , vol.17 , pp. 6545-6551
    • Hemsley, A.1    Arnheim, N.2    Toney, M.D.3    Cortopassi, G.4    Galas, D.J.5
  • 16
  • 17
    • 0028856773 scopus 로고
    • Efficient amplification using 'megaprimer' by asymmetric polymerase chain reaction
    • Datta, A. K. (1995) Efficient amplification using 'megaprimer' by asymmetric polymerase chain reaction. Nucleic Acids Res. 23, 4530-4531.
    • (1995) Nucleic Acids Res. , vol.23 , pp. 4530-4531
    • Datta, A.K.1
  • 18
    • 0025643362 scopus 로고
    • A general method for rapid site-directed mutagenesis using the polymerase chain reaction
    • Landt, O., Grunert, H. P., and Hahn, U. (1990) A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96, 125-128.
    • (1990) Gene , vol.96 , pp. 125-128
    • Landt, O.1    Grunert, H.P.2    Hahn, U.3
  • 19
    • 0030743251 scopus 로고    scopus 로고
    • Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method
    • Ke, S. H. and Madison, E. L. (1997) Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method. Nucleic Acids Res. 25, 3371-3372.
    • (1997) Nucleic Acids Res. , vol.25 , pp. 3371-3372
    • Ke, S.H.1    Madison, E.L.2
  • 21
    • 0026729267 scopus 로고
    • Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme
    • Lowenstein, C. J., Glatt, C. S., Bredt, D. S., and Snyder, S. H. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proc. Natl. Acad. Sci. USA 89, 6711-6715.
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 6711-6715
    • Lowenstein, C.J.1    Glatt, C.S.2    Bredt, D.S.3    Snyder, S.H.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.