Generation of large deletion mutants from plasmid DNA

BioTechniques

Volumn 36, Issue 3, 2004, Pages 402-406

  Stoynova, Ludmila ^a   Solórzano, Ramona ^a   Collins, Elaine D ^a  

^a SAN JOSE STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; EXONUCLEASE; MACROGOL; MUTANT PROTEIN; PLASMID DNA; VITAMIN D RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME METABOLISM; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE DELETION; GENE MUTATION; GENE SEQUENCE; NONHUMAN; POLYMERASE CHAIN REACTION; WILD TYPE;

ESCHERICHIA COLI;

EID: 1542285933     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04363bm05     Document Type: Article

References (8)

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3. Eckstein, F. and G. Gish. 1989. Phosphorothioates in molecular biology. Trends Biochem. Sci. 14:97-100.
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5. Kerr, C. and P.D. Sadowski. 1972. Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction. J. Biol. Chem. 247: 311-318.
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8. da Costa, L.J. and A. Tanuri. 1998. Use of T7 gene 6 exonuclease and phosphorothioated primers for the manipulation of HIV-1 infectious clones. J. Virol. Methods 72: 117-121.