Economical use of substrates in enzyme activity blots

American Biotechnology Laboratory

Volumn 16, Issue 13, 1998, Pages 20-

  Oppert, Brenda ^a   Kramer, Karl J ^a  

^a GRAIN MARKETING AND PRODUCTION RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 29444437113     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Ohlsson BG, Westrom BR, Karlsson BW. Enzymoblotting: a method for localizing proteinases and their zymogens using para-nitroanilide substrates after agarose gel electrophoresis and transfer to nitrocellulose. Anal Biochem 1986; 152:239-14.
2. Oppert B, Kramer KJ, Johnson D, Upton SJ, McGaughey WH. Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CrylA(c) protoxin. Insect Biochem Molec Biol 1996; 26:571-83.
3. 4. Nature (London) 1970; 227:680-5.
4. Oppert B, Kramer KJ, McGaughey WH. Rapid microplate assay of protetnase mixtures. Bio Techniques 1997; 23: 70-2.
5. Oppert B, Kramer KJ, Beeman RW, Johnson D, McGaughey WH. Proteinasc-mediated resistance to Bacillus thuringiensis insecticidal toxins. J Biol Chem 1997; 272:23473-6.