Rapid microplate assay for substrates and inhibitors of proteinase mixtures

BioTechniques

Volumn 23, Issue 1, 1997, Pages 70-72

  Oppert, Brenda ^a,b   Kramer, Karl J ^a   McGaughey, William H ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

ARTICLE; COLORIMETRY; DATA ANALYSIS; ENZYME INHIBITION; HYDROLYSIS; PROTEIN ANALYSIS;

EID: 0030758530     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm14     Document Type: Article

References (3)

1. Laura, R., D.J. Robison and D.H. Bing. 1980. (p-Amidinophenyl) methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry 19:4859-4864.
2. Little, S.E. and J.T. Little. 1995. Rapid protease screening using ammonium sulfate precipitations and a high-performance centrifuge. Am. Biotechnol. Lab. July, p. 28.
3. Oppert, B., K.J. Kramer, D. Johnson, S.J. Upton and W.H. McGaughey. 1996. Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin. Insect Biochem. Mol. Biol. 26:571-583.