DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings

Nucleic Acids Research

Volumn 32, Issue 10, 2004, Pages 2977-2986

  Thirlway, Jenny ^a   Turner, Ian J ^a   Gibson, Christopher T ^a   Gardiner, Laurence ^a   Brady, Kevin ^a   Allen, Stephanie ^a   Roberts, Clive J ^a   Soultanas, Panos ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA PRIMASE; HELICASE; BACTERIAL PROTEIN; DNAB HELICASE;

AMINO TERMINAL SEQUENCE; ARTICLE; ATOMIC FORCE MICROSCOPY; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME BINDING; ENZYME STABILITY; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN POLYMERIZATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; DNA REPLICATION; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; ULTRASTRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

BACILLUS STEAROTHERMOPHILUS; BACTERIAL PROTEINS; DNA HELICASES; DNA PRIMASE; DNA REPLICATION; DNAB HELICASES; MICROSCOPY, ATOMIC FORCE; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY;

EID: 2942679433     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkh628     Document Type: Article

References (45)

1. Caruthers,J.M. and McKay,D.B. (2002) Helicase structure and mechanism. Curr. Opin. Struct. Biol., 12, 123-133.
2. von Hippel,P.H. and Delagoute,E. (2001) A general model for nucleic acid helicases and their 'coupling' within macromolecular machines. Cell, 104, 177-190.
3. Soultanas,P. and Wigley,D.B. (2000) DNA helicases: 'inching forward'. Curr. Opin. Struct. Biol., 10, 124-128.
4. Patel,S.S. and Picha,K.M. (2000) Structure and function of hexameric helicases. Annu. Rev. Biochem., 69, 651-697.
5. Katayama,T., Kubota,T., Kurokawa,K., Crooke,E. and Sekimizu,K. (1998) The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E.coli chromosomal replicase. Cell, 94, 61-71.
6. Datta,H.J., Khatri,G.S. and Bastia,D. (1999) Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101. Proc. Natl Acad. Sci. USA, 96, 73-78.
7. Konieczny,I. and Marszalek,J. (1995) The requirement for molecular chaperones in lambda-DNA replication is reduced by the mutation-Pi in lambda-P gene, which weakens the interaction between lambda-P protein and DnaB helicase. J. Biol. Chem., 270, 9792-9799.
8. Wahle,E., Lasken,R.S. and Kornberg,A. (1989) The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties. J. Biol. Chem., 264, 2463-2468.
9. Wahle,E., Lasken,R.S. and Kornberg,A. (1989) The dnaB-dnaC replication protein complex of Escherichia coli. II. Role of the complex in mobilizing dnaB functions. J. Biol. Chem., 264, 2469-2475.
10. Imai,Y., Ogasawara,N., Ishigo-Oka,D., Kadoya,R., Daito,T. and Moriya,S. (2000) Subcellular localization of DNA-initiation protein of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids. Mol. Microbiol., 36, 1037-1048.
11. Soultanas,P. (2002) A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus. Nucleic Acids Res., 30, 966-974.
12. Smelkova,N. and Marians,K.J. (2001) Timely release of both replication forks from oriC requires modulation of origin topology. J. Biol. Chem., 276, 39186-39191.
13. Kim,S., Dallmann,H.G., McHenry,C.S. and Marians,K.J. (1996) Coupling of a replicative polymerase and helicase: a τ-DnaB interaction mediates rapid replication fork movement. Cell, 84, 643-650.
14. Haroniti,A., Till,R., Smith,M.C.M. and Soultanas,P. (2003) The clamp-loader-helicase interaction in Bacillus. Leucine 381 is critical for pentamerisation and helicase-binding of the Bacillus τ protein. Biochemistry, 42, 10955-10964.
15. Haroniti,A., Anderson,C., Doddridge,Z., Gardiner,L., Roberts,C.J., Allen,S. and Soultanas,P. (2004) The clamp-loader-helicase interaction in Bacillus. Atomic force microscopy reveals the structural organization of the τ-DnaB complex in Bacillus. J. Mol. Biol., 336, 381-393.
16. Bussiere,D.E., Bastia,D. and White,S.W. (1995) Crystal-structure of the replication terminator protein from Bacillus-subtilis at 2.6-angstrom. Cell, 80, 651-660.
17. Gautam,A., Mulugu,S., Alexander,K. and Bastia,D. (2001) A single domain of the replication termination protein of Bacillus subtilis is involved in arresting both DnaB helicase and RNA polymerase. J. Biol. Chem., 276, 23471-23479.
18. Tougu,K and Marians,K.J. (1996) The interaction between helicase and primase sets the replication fork clock. J. Biol. Chem., 271, 21391-21397.
19. Bird,L.E., Pan,H., Soultanas,P. and Wigley,D.B. (2000) Mapping protein-protein interactions within a stable complex of DNA primase and DnaB helicase from Bacillus stearothermophilus. Biochemistry, 39, 171-182.
20. Ng,J.Y. and Marians,K.J. (1996) The ordered assembly of the ΦX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes. J. Biol. Chem., 271, 15642-15648.
21. Ng,J.Y and Marians,K.J. (1996) The ordered assembly of the ΦX174-type primosome. II. Preservation of primosome composition from assembly through replication. J. Biol. Chem., 271, 15649-15655.
22. Liu,J., Nurse,P. and Marians,K.J. (1996) The ordered assembly of the ΦX174-type primosome. III. PriB facilitates complex formation between PriA and DnaT. J. Biol. Chem., 271, 15656-15661.
23. Chang,P. and Marians,K.J. (2000) Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork. J. Biol. Chem., 275, 26187-26195.
24. Maurer,R. and Wong,A. (1988) Dominant-lethal mutations in the dnaB helicase gene of Salmonella typhimurium. J. Bacteriol., 170, 3682-3688.
25. Stordal,L. and Maurer,R. (1996) Defect in general priming conferred by linker region mutants of Escherichia coli DnaB. J. Bacteriol., 178, 4620-4627.
26. Bird,L.E. and Wigley,D.B. (1999) The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity. Biochim. Biophys. Acta, 1444, 424-428.
27. Lu,Y.B., Ratnakar,P., Mohanty,B.K. and Bastia,D. (1996) Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA. Proc. Natl Acad. Sci. USA, 93, 12902-12907.
28. Fass,D., Bogden,C.E. and Berger,J.M. (1999) Crystal structure of the N-terminal domain of the DnaB hexameric helicase. Structure, 7, 691-698.
29. Weigelt,J., Brown,S.E., Miles,C.S., Dixon,N.E. and Otting,G. (1999) NMR structure of the N-terminal domain of E.coli DnaB helicase: implications for structure rearrangements in the helicase hexamer. Structure, 7, 681-690.
30. Sawaya,M.R., Guo,S., Tabor,S., Richardson,C.C. and Ellenberger,T. (1999) Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7. Cell, 99, 167-177.
31. Singleton,M.R., Sawaya,M.R., Ellenberger,T. and Wigley,D.B. (2000) Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides. Cell, 101, 589-600.
32. Niedenzu,T., Roleke,D., Bains,G., Scherzinger,E. and Saenger,W. (2001) Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010. J. Mol. Biol., 306, 479-487.
33. Soultanas,P. and Wigley,D.B. (2002) Site-directed mutagenesis reveals roles for conserved amino acid residues in the hexameric DNA helicase DnaB from Bacillus stearothermophilus. Nucleic Acids Res., 30, 4051-4060.
34. Pullman,M.E., Penefsky,A., Datta,A. and Racker,E. (1960) Partial resolution of the enzymes catalysing oxidative phosphorylation. J. Biol. Chem., 235, 3322-3329.
35. Becker,D.M. and Guarente,L. (1991) High-efficiency transformation of yeast by electroporation. Methods Enzymol., 194, 182-187.
36. Dumay,H., Rubbi,L., Sentenac,A. and Marck,C. (1999) Interaction between yeast RNA polymerase III and transcription factor TFIIIC via ABC10α and τ131 subunits. J. Biol. Chem., 274, 33462-33468.
37. Biswas,S.B., Chen,P.H. and Biswas,E.E. (1994) Structure and function of Escherichia coli DnaB protein: role of the N-terminal domain in helicase activity. Biochemistry, 33, 11307-11314.
38. Yang,S., Yu,X., VanLoock,M.S., Jezewska,M.J., Bujalowski,W. and Egelman,E.H. (2002) Flexibility of the rings: structural asymmetry in the DnaB hexameric helicase. J. Mol. Biol., 321, 839-849.
39. Bird,L.E., Hakansson,K., Pan,H. and Wigley,D.B. (1997) Characterisation and crystallisation of the helicase domain of bacteriophage T7 gene 4 protein. Nucleic Acids Res., 25, 2620-2626.
40. Frick,D.N. and Richardson,C.C. (2001) DNA primases. Annu. Rev. Biochem., 70, 39-80.
41. Yuzhakov,A., Kelman,Z. and O'Donnell,M. (1999) Trading places on DNA - a three-point switch underlies primer handoff from primase to the replicative DNA polymerase. Cell, 96, 153-163.
42. Pan,H. and Wigley,D.B. (2000) Structure of the zinc-binding domain of Bacillus stearothermophilus DNA primase. Structure, 8, 231-239.
43. Keck,J.L., Roche,D.D., Lynch,A.S. and Berger,J.M. (2000) Structure of the RNA polymerase domain of E.coli primase. Science, 287, 2482-2486.
44. Van Loock,M.S., Chen,Y.-J., Yu,X., Patel,S.S. and Egelman,E.H. (2001) The primase active site is on the outside of the hexameric bacteriophage T7 gene 4 helicase-primase ring. J. Mol. Biol., 311, 951-956.
45. Hingorani,M.M., Washington,M.T., Moore,K.C. and Patel,S.S. (1996) The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase. Proc. Natl Acad. Sci. USA, 94, 5012-5017.