Reactive amino acid residues involved in glutamate-binding of human glutamate dehydrogenase isozymes

Biochimie

Volumn 86, Issue 4-5, 2004, Pages 261-267

  Yoon, Hye Young ^a,d   Cho, Eun Hee ^b   Yang, Seung Ju ^a,c   Lee, Hyun Ju ^a   Huh, Jae Wan ^a   Choi, Myung Min ^a   Cho, Sung Woo ^a  

^a University of Ulsan College of Medicine   (South Korea)

^b Chosun University   (South Korea)

^c Yonsei University Wonju   (South Korea)

^d NATIONAL CANCER INSTITUTE   (United States)

Author keywords

GDH; Glutamate dehydrogenase; glutamate dehydrogenase; Glutamate binding site; Isozymes; Reactive glycine; Reactive lysine

Indexed keywords

ADENOSINE DIPHOSPHATE; AMINO ACID; ARACHIDONIC ACID; GLUTAMATE DEHYDROGENASE; GUANOSINE TRIPHOSPHATE; ISOENZYME; MUTANT PROTEIN;

ANIMAL CELL; ARTICLE; BINDING KINETICS; BINDING SITE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME BINDING; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GENE CASSETTE; HUMAN; HUMAN CELL; MUTAGENESIS; NONHUMAN; PROTEIN STABILITY; SENSITIVITY ANALYSIS; WILD TYPE;

ADENOSINE DIPHOSPHATE; AMINO ACIDS; BINDING SITES; CATALYSIS; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; GUANOSINE TRIPHOSPHATE; HUMANS; ISOENZYMES; MUTAGENESIS, SITE-DIRECTED; MUTATION;

EID: 2942655190     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biochi.2004.04.005     Document Type: Article

References (46)

1. Smith E., Austin B., Blumenthal K., Nyc J. Glutamate dehydrogenase. Boyer P. The Enzymes. 11:1975;293-367 Academic Press, New York
2. Fisher H.F. L-Glutamate dehydrogenase from bovine liver. Methods Enzymol. 113:1985;16-27
3. Hudson R.C., Daniel R.M. L-Glutamate dehydrogenase: distribution, properties and mechanism. Comp. Biochem. Physiol. B. 106:1993;767-792
4. Nissim I. Newer aspects of glutamine/glutamate metabolism: the role of acute pH changes. Am. J. Physiol. 277:1999;F493-F497
5. Plaitakis A., Berl S., Yahr M.D. Neurological disorders associated with deficiency of glutamate dehydrogenase. Ann. Neurol. 15:1984;144-153
6. Hussain M.H., Zannis V.I., Plaitakis A. Characterization of glutamate dehydrogenase isoproteins purified from the cerebellum of normal subjects and patients with degenerative neurological disorders and from human neoplastic cell lines. J. Biol. Chem. 264:1989;20730-20735
7. McDaniel H.G. Comparison of the primary structure of nuclear and mitochondrial glutamate dehydrogenase from bovine liver. Arch. Biochem. Biophys. 319:1995;316-321
8. Rajas F., Gire V., Rouset B. Involvement of a membrane-bound form of glutamate dehydrogenase in the association of lysosomes to microtubules. J. Biol. Chem. 271:1996;29882-29890
9. Stanley C.A., Lieu Y.K., Hsu B.Y.L., Burlina A.B., Greenberg C.R., Hopwood N.J., Perlman K., Rich B.H., Zammarchi E., Poncz M. Hyperinsulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene. New Engl. J. Med. 338:1988;1353-1357
10. Tanizawa Y., Nakai K., Sasaki T., Anno T., Ohta Y., Inoue H., Matsuo K., Koga M., Furukawa S., Oka Y. Unregulated elevation of glutamate dehydrogenase activity induces glutamine-stimulated insulin secretion: identification and characterization of a GLUD1 gene mutation and insulin secretion studies with MIN6 cells overexpressing the mutant glutamate dehydrogenase. Diabetes. 51:2002;712-717
11. Yorifuji T., Muroi J., Uematsu A., Hiramatsu H., Momoi T. Hyperinsulinism-hyperammonemia syndrome caused by mutant glutamate dehydrogenase accompanied by novel enzyme kinetics. Hum. Genet. 104:1999;476-479
12. Yang S.-J., Huh J.-W., Kim M.J., Lee W.-J., Kim T.U., Choi S.Y., Cho S.-W. Regulatory effects of 5'-deoxypyridoxal on glutamate dehydrogenase activity and insulin secretion in pancreatic islets. Biochimie. 85:2003;581-586
13. Cho S.-W., Ahn J.-Y., Lee J., Choi S.Y. Identification of a peptide of the guanosine triphosphate binding site within brain glutamate dehydrogenase isoproteins using 8-azidoguanosine triphosphate. Biochemistry. 35:1996;13907-13913
14. Rice D.W., Baker P.J., Farrants G.W., Hornby D.P. The crystal structure of glutamate dehydrogenase from Clostridium symbiosum at 0.6 nm resolution. Biochem. J. 242:1987;789-795
15. Knapp S., De Vos W.M., Rice D., Ladenstein R. Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 Å resolution. J. Mol. Biol. 267:1997;916-932
16. Stillman T.J., Baker P.J., Britton K.L., Rice D.W. Conformational flexibility in glutamate dehydrogenase: role of water in substrate recognition and catalysis. J. Mol. Biol. 234:1993;1131-1139
17. Stillman T.J., Migueis A.M.B., Wang X.-G., Baker P.J., Britton K.L., Engel P.C., Rice D.W. Insights into the mechanism of domain closure and substrate specificity of glutamate dehydrogenase from Clostridium symbiosum. J. Mol. Biol. 285:1999;875-885
18. Peterson P.E., Smith T.J. The structure of bovine glutamate dehydrogenase provides insights into the mechanism of allostery. Structure. 7:1999;769-782
19. Smith T.J., Peterson P.E., Schmidt T., Fang J., Stanley C.A. Structure of bovine glutamate dehydrogenase complexes elucidate the mechanism of purine regulation. J. Mol. Biol. 307:2001;707-720
20. Smith T.J., Schmidt T., Fang J., Wu J., Siuzdak G., Stanley C.A. The structure of apo human glutamate dehydrogenase details subunit communication and allostery. J. Mol. Biol. 318:2002;765-777
21. Hochreiter M.C., Schellenberg K.A. Alpha-iminoglutarate formation by beef liver L-glutamate dehydrogenase. Detection by borohydride of dithionite reduction to glutamate. J. Am. Chem. Soc. 91:1969;6530-6538
22. Rife J.E., Cleland W.W. Determination of the chemical mechanism of glutamate dehydrogenase from pH studies. Biochemistry. 19:1980;2321-2328
23. Chen S.-S., Engel P.C. Ox liver glutamate dehydrogenase. The role of Kine-126 reappraised in the light of studies of inhibition and inactivation by pyridoxal 5'-phosphate. Biochem. J. 149:1995;619-626
24. McPherson M.J., Baron A.J., Jones K.M., Price G.J., Wootton J.C. Multiple interactions of Kine-128 of Escherichia coli glutamate dehydrogenase revealed by site-directed mutagenesis studies. Protein Eng. 2:1988;147-152
25. +-dependent glutamate dehydrogenase of Clostridium symbiosum. Biochim. Biophys. Acta. 1080:1991;191-197
26. Dean J.L.E., Wang X.-G., Teller J.K., Waugh M.L., Britton L.K., Baker P.J., Stillman T.J., Martin S.M., Rice D.W., Engel P.C. The catalytic role of aspartate in the active site of glutamate dehydrogenase. Biochem. J. 301:1994;13-16
27. Baker P.J., Britton K.L., Rice D.W., Rob A., Stillman T.J. Structural consequences of sequence patterns in the fingerprint region of the nucleotide binding fold implications for nucleotide specificity. J. Mol. Biol. 228:1992;662-671
28. Teller J.K., Smith R.J., McPherson M.J., Engel P.C., Guest J.R. Correlation of intron-exon organization with the three-dimensional structure in glutamate dehydrogenase. Eur. J. Biochem. 206:1992;151-159
29. Cho S.-W., Yoon H.-Y., Ahn J.-Y., Lee E.-Y., Lee J. Cassette mutagenesis of lysine 130 of human glutamate dehydrogenase: an essential residue in cataKis. Eur. J. Biochem. 268:2001;3205-3213
30. Lee E.Y., Yoon H.Y., Ahn J.-Y., Choi S.Y., Cho S.-W. Identification of GTP binding site of human glutamate dehydrogenase using cassette mutagenesis and photoaffinity labeling. J. Biol. Chem. 276:2001;47930-47936
31. Yoon H.-Y., Lee E.-Y., Cho S.-W. Cassette mutagenesis and photoaffinity labeling of adenine binding domain of ADP regulatory site within human glutamate dehydrogenase. Biochemistry. 41:2002;6817-6823
32. Yang S.-J., Huh J.-W., Hong H.-N., Kim T.U., Cho S.-W. Important role of Ser443 in different thermal stability of human glutamate dehydrogenase isozymes. FEBS Lett. 562:2004;59-64
33. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:1983;557-580
34. Studier F.W., Moffatt B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:1986;113-130
35. Choi S.Y., Hong J.W., Song M.-S., Jeon S.G., Bahn J.H., Lee B.Y., Ahn J.-Y., Cho S.-W. Different antigenic reactivities of bovine brain glutamate dehydrogenase isoproteins. J. Neurochem. 72:1999;2162-2169
36. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
37. Cho S.-W., Lee J., Choi S.Y. Two soluble forms of glutamate dehydrogenase isoproteins from bovine brain. Eur. J. Biochem. 233:1995;340-346
38. Fersht A. Enzyme Structure and Mechanism. 1985;98-120 W.H. Freeman, New York
39. Valinger Z., Engel P.C., Metzler D.E. Is pyridoxal 5'-phosphate an affinity label for phosphate-binding sites in proteins? the case of bovine glutamate dehydrogenase. Biochem. J. 294:1993;835-839
40. Kim S.W., Lee J., Song M.-S., Choi S.Y., Cho S.-W. Essential active-site lysine of brain glutamate dehydrogenase isoproteins. J. Neurochem. 69:1997;418-422
41. Singh N., Maniscalco S., Fisher H. The real-time resolution of proton-related transient-state steps in an enzymatic reaction. The early steps in the oxidative deamination reaction of bovine liver glutamate dehydrogenase. J. Biol. Chem. 268:1993;21-28
42. Shashidharan P., Michaelidis T.M., Robakis N.K., Kresovali A., Papamatheakis J., Pliatakis A. Novel human glutamate dehydrogenase expressed in neural and testicular tissues and encoded by an X-linked intronless gene. J. Biol. Chem. 269:1994;16971-16976
43. Shashidharan P., Clarke D.D., Ahmed N., Moschonas N., Plaitakis A. Nerve tissue-specific human glutamate dehydrogenase that is thermolabile and highly regulated by ADP. J. Neurochem. 68:1997;1804-1811
44. Zaganas I., Plaitakis A. Single amino acid substitution (G456A) in the vicinity of the GTP binding domain of human housekeeping glutamate dehydrogenase markedly attenuates GTP inhibition and abolishes the cooperative behavior of the enzyme. J. Biol. Chem. 277:2002;26422-26428
45. Plaitakis A., Spanaki C., Mastorodemos V., Zaganas I. Study of structure-function relationships in human glutamate dehydrogenases reveals novel molecular mechanisms for the regulation of the nerve tissue-specific (GLUD2) isoenzyme. Neurochem. Int. 43:2003;401-410
46. Cho S.-W., Yoon H.-Y. Photoaffinity labeling of brain glutamate dehydrogenase isoproteins with an azido-ADP. J. Biol. Chem. 274:1999;13948-13953