Endogenous glutathione and catalase protect cultured rat astrocytes from the iron-mediated toxicity of hydrogen peroxide

Neuroscience Letters

Volumn 364, Issue 3, 2004, Pages 164-167

  Liddell, Jeff R ^a   Robinson, Stephen R ^a   Dringen, Ralf ^a,b  

^a MONASH UNIVERSITY   (Australia)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Astrocytes; Catalase; Glutathione; Hydrogen peroxide; Iron; Oxidative stress

Indexed keywords

AMITROLE; BUTHIONINE SULFOXIMINE; CATALASE; DEFEROXAMINE; GLUTATHIONE; HYDROGEN PEROXIDE; IRON; LACTATE DEHYDROGENASE;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN PROTECTION; BRAIN TISSUE; CELL CULTURE; CELL KILLING; CELL LOSS; CELL PROTECTION; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; DETOXIFICATION; DRUG EFFECT; NEUROTOXICITY; NEWBORN; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT;

ANIMALS; ASTROCYTES; CATALASE; CELLS, CULTURED; GLUTATHIONE; HYDROGEN PEROXIDE; IRON; OXIDANTS; OXIDATIVE STRESS; RATS;

EID: 2942627809     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2004.04.042     Document Type: Article

References (17)

1. Abe K., Saito H. Characterization of t-butyl hydroperoxide toxicity in cultured cortical neurones and astrocytes. Pharmacol. Toxicol. 83:1998;40-46
2. H.E. Aebi, Catalase, in: H.U. Bergmeyer, J. Bergmeyer, M. Grassl (Eds.), Methods of Enzymatic Analysis, vol. 3, Verlag Chemie, Weinheim, Germany, 1984, pp. 273-286.
3. Crichton R.R., Wilmet S., Legssyer R., Ward R.J. Molecular and cellular mechanisms of iron homeostasis and toxicity in mammalian cells. J. Inorg. Biochem. 91:2002;9-18
4. Dringen R. Metabolism and functions of glutathione in brain. Prog. Neurobiol. 62:2000;649-671
5. Dringen R., Hamprecht B. Involvement of glutathione peroxidase and catalase in the disposal of exogenous hydrogen peroxide by cultured astroglial cells. Brain Res. 759:1997;67-75
6. Dringen R., Kussmaul L., Hamprecht B. Detoxification of exogenous hydrogen peroxide and organic hydroperoxides by cultured astroglial cells assessed by a microtiter plate assay. Brain Res. Protoc. 2:1998;223-228
7. Dringen R., Kussmaul L., Gutterer J.M., Hirrlinger J., Hamprecht B. The glutathione system of peroxide detoxification is less efficient in neurons than in astroglial cells. J. Neurochem. 72:1999;2523-2530
8. Hamprecht B., Löffler F. Primary glial cultures as a model system for studying hormone action. Methods Enzymol. 109:1985;341-345
9. Hirrlinger J., Resch A., Gutterer J.M., Dringen R. Oligodendroglial cells in culture effectively dispose of exogenous hydrogen peroxide: comparison with cultured neurones, astroglial and microglial cells. J. Neurochem. 82:2002;635-644
10. Hoepken H.H., Korten T., Robinson S.R., Dringen R. Iron accumulation, iron-mediated toxicity and altered levels of ferritin and transferrin receptor in cultured astrocytes during incubation with ferric ammonium citrate. J. Neurochem. 88:2004;1194-1202
11. 2 in vitro. Brain Res. 671:1995;181-186
12. Jenner P. Oxidative stress in Parkinson's disease. Ann. Neurol. 53(Suppl. 3):2003;S26-S36
13. Kruszewski M. Labile iron pool: the main determinant of cellular response to oxidative stress. Mutat. Res. 531:2003;81-92
14. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275
15. Milton N.G. Role of hydrogen peroxide in the aethiology of Alzheimer's disease: implications for treatment. Drugs Aging. 21:2004;81-100
16. Robb S.J., Connor J.R. An in vitro model for analysis of oxidative death in primary mouse astrocytes. Brain Res. 788:1998;125-132
17. Ying W., Han S.K., Miller J.W., Swanson R.A. Acidosis potentiates oxidative neuronal death by multiple mechanisms. J. Neurochem. 73:1999;1549-1556