Silver staining of proteins on electroblotting membranes and intensification of silver staining of proteins separated by polyacrylamide gel electrophoresis

Analytical Biochemistry

Volumn 304, Issue 1, 2002, Pages 33-41

  Sørensen, Birgitte Kjær ^a   Højrup, Peter ^b   Østergård, Erik ^a   Jørgensen, Charlotte Sværke ^a   Enghild, Jan ^c   Ryder, Lisa Rebekka ^a   Houen, Gunnar ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^c AARHUS UNIVERSITY   (Denmark)

Author keywords

Electrophoresis; Gallyas' stain; Mass spectrometry; Proteins; Silver intensification

Indexed keywords

ELECTROPHORESIS; ENDOTHELIAL CELLS; GELS; MASS SPECTROMETRY; MEMBRANES; SEPARATION; SIGNAL TO NOISE RATIO; SILVER;

ELECTROPHORESIS GELS; FULLY COMPATIBLE; GALLYAS' STAIN; HUMAN ENDOTHELIAL CELLS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SILVER STAINING; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PROTEINS;

CELL PROTEIN; FLUORIDE; MEMBRANE PROTEIN; POLYVINYL DERIVATIVE; SILVER;

ANALYTIC METHOD; ARTICLE; ELECTROBLOTTING; ENDOTHELIUM CELL; GEL; HISTOCHEMISTRY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO; SILVER STAINING;

EID: 0036571319     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5604     Document Type: Article

References (30)

1. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels
2. Silver-staining of proteins in polyacrylamide gels: A general overview
3. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
4. Staining and quantification of proteins separated by polyacrylamide gel electrophoresis
5. Mechanisms of protein silver staining in polyacrylamide gels: A 10-year synthesis
6. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry
7. Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes
8. A silver stain for the rapid quantitative detection of proteins or nucleic acids on membranes or thin layer plates
9. An improved colloidal silver staining method of protein blots on nitrocellulose membranes
10. Colloidal silver staining of electroblotted proteins for high sensitivity peptide mapping by liquid chromatography-electrospray ionization tandem mass spectrometry
11. Comparison of different staining methods for polyvinylidene difluoride membranes
12. Suppression of the argyrophil III reaction by mercapto compounds (a prerequisite for the intensification of certain histochemical reactions by physical developers)
13. High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry
14. Application of the silver-gold intensified 3,3′-diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain
15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
16. Methods for increasing the resolution of two-dimensional protein electrophoresis
17. The current state of two-dimensional electrophoresis with immobilized pH gradients
18. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
19. Mechanisms of protein silver staining in polyacrylamide gels: A 10-year synthesis
20. Ammoniacal silver staining of proteins: Mechanism of glutaraldehyde enhancement
21. Increased sensitivity in peroxidase immunocytochemistry. A comparative study of a number of peroxidase visualization methods employing a model system
22. Routine identification of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS)
23. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity
24. Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels
25. Colloidal silver staining of electroblotted proteins for high sensitivity peptide mapping by liquid chromatography-electrospray ionization tandem mass spectrometry
26. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry
27. Matrix-assisted laser desorption/ionisation mass spectrometry: Sample preparation techniques designed for various peptide and protein analytes