Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans

Comparative and Functional Genomics

Volumn 5, Issue 3, 2004, Pages 225-229

  van der Linden, Alexander M ^a   Plasterk, Ronald H A ^a  

^a HUBRECHT INSTITUTE   (Netherlands)

Author keywords

Caenorhabditis elegans; Insertional mutagenesis screen; mut 7; Tc1; Transposon insertion display protocol

Indexed keywords

CAENORHABDITIS ELEGANS PROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; GENE IDENTIFICATION; GENE INSERTION; GENE MAPPING; GENE SEQUENCE; GENETIC SCREENING; GERM LINE; MOLECULAR CLONING; MULTIGENE FAMILY; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; INSERTION SEQUENCES; TRANSPOSONS;

EID: 2942534291     PISSN: 15316912     EISSN: None     Source Type: Journal    
DOI: 10.1002/cfg.392     Document Type: Article

References (19)

1. C. elegans BLAST server: http://www.sanger.ac.uk/Projects/C_elegans/blast_server.shtml
2. Consortium. 1998. Genome sequence of the nematode C. elegans: a platform for investigating biology. The C. elegans Sequencing Consortium. Science 282: 2012-2018.
3. Fischer SEJ, Wienholds E, Plasterk RHA. 2003. Continuous exchange of sequence information between dispersed Tc1 transposons in the C. elegans genome. Genetics 164: 127-134.
4. Kamath RS, Fraser AG, Dong Y. et al., 2003. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421: 231-237.
5. Ketting RF, Haverkamp TH, van Luenen HG, Plasterk RH. 1999. Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99: 133-141.
6. Korswagen HC, Durbin RM, Smits MT, Plasterk RHA. 1996. Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markets for gene mapping. Proc Natl Acad Sci USA 93: 14 680-14 685.
7. Lewis JA, Fleming JT. 1995. Basic culture methods. Methods Cell Biol 48: 3-29.
8. Liao GC, Rehm EJ, Rubin GM. 2000. Insertion site preferences of the P transposable element in Drosophila melanogaster. Proc Natl Acad Sci USA 97: 3347-3351.
9. Martin E, Laloux H, Couette G, et al., 2002. Identification of 1088 new transposon insertions of Caenorhabditis elegans: a pilot study toward large-scale screens. Genetics 162: 521-524.
10. Plasterk RHA, van Luenen H. 1997. Transposons. In C. elegans II, Riddle D, Blumenthal T, Meyer B, Priess J (eds). Cold Spring Harbor Laboratory Press: New York; 97-116.
11. Rushforth AM, Anderson P. 1996. Splicing removes the Caenorhabditis elegans transposon Tc1 from most mutant pre-mRNAs. Mol Cell Biol 16: 422-429.
12. Transposon Insertion Display Protocol: http://www.niob.knaw.nl.researchpages/plasterk/transdip.html
13. Vidan S, Snyder M. 2001. Large-scale mutagenesis: yeast genetics in the genome era. Curr Opin Biotechnol 12: 28-34.
14. Waterston RH, Sulston JE, Coulson AR. 1997. The genome. In C. elegans II, Riddle D, Blumenthal T, Meyer B, Priess J (eds). Cold Spring Harbor Laboratory Press: New York; 23-45.
15. Wicks SR, de Vries CJ, van Luenen HG, Plasterk RH. 2000. CHE-3, a cytosolic dynein heavy chain, is required for sensory cilia structure and function in Caenorhabditis elegans. Dev Biol 221: 295-307.
16. Williams BD, Schrank B, Huynh C, Shownkeen R, Waterston RH. 1992. A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131: 609-624.
17. Wormbase Database: http://www.wormbase.org
18. Zambrowicz BP, Friedrich GA, Buxton EC, et al. 1998. Disruption and sequence identification of 2000 genes in mouse embryonic stem cells. Nature 392: 608-611.
19. Zwaal RR, Broeks A, van Meurs J, Groenen JT, Plasterk RH. 1993. Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank. Proc Natl Acad Sci USA 90: 7431-7435.