Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

Applied and Environmental Microbiology

Volumn 70, Issue 6, 2004, Pages 3769-3771

  Abdullah, Mohd Amir F ^a,b   Dean, Donald H ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; MUTAGENESIS; PROTEINS; TOXICITY;

MUTATIONS; PROTEIN ENGINEERING;

MICROBIOLOGY;

BACILLUS THURINGIENSIS TOXIN; PROTEIN CRY19AA; UNCLASSIFIED DRUG;

MICROBIOLOGY;

AEDES AEGYPTI; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; CRY19AA GENE; CULEX; GENE MUTATION; NONHUMAN; PROTEIN DOMAIN; PROTEIN ENGINEERING; TOXICITY TESTING;

AEDES; AMINO ACID SEQUENCE; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; ENDOTOXINS; HEMOLYSIN PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PEST CONTROL, BIOLOGICAL; PROTEIN ENGINEERING;

AEDES AEGYPTI; ARACHNIDA; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); HEXAPODA; POSIBACTERIA;

EID: 2942532173     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.6.3769-3771.2004     Document Type: Article

References (11)

1. Abdullah, M. A. F., O. Alzate, M. Mohammad, R. J. McNall, M. J. Adang, and D. H. Dean. 2003. Introduction of Culex toxicity into Bacillus thuringiensis Cry4Ba by protein engineering. Appl. Environ. Microbiol. 69:5343-5353.
2. Dean, D. H., F. Rajamohan, M. K. Lee, S.-J. Wu, X.-J. Chen, E. Alcantara, and S. R. Hussain. 1996. Probing the mechanism of action of Bacillus thuringiensis insecticidal proteins by site-directed mutagenesis. Gene 179:111-117.
3. Guex, N., and M. C. Peitsch. 1997. SWISS-MODEL and the Swiss-Pdb Viewer: an environment for comparative protein modeling. Electrophoresis 18:2714-2723.
4. Lu, H., F. Rajamohan, and D. H. Dean. 1994. Identification of amino acid residues of Bacillus thuringiensis δ-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. J. Bacteriol. 176:5554-5559.
5. Peitsch, M. C. 1996. ProMod and Swiss-Model: internet-based tools for automated comparative protein modelling. Biochem. Soc. Trans. 24:274-279.
6. Peitsch, M. C. 1995. Protein modelling by e-mail. Bio/Technology 13:658-660.
7. Ragni, A., I. Thiéry, and A. Delécluse. 1996. Characterization of six highly mosquitocidal Bacillus thuringiensis strains that do not belong to the H-14 serotype. Curr. Microbiol. 32:48-54.
8. Rosso, M. L., and A. Delecluse. 1997. Contribution of the 65-kilodalton protein encoded by the cloned gene cry19A to the mosquitocidal activity of Bacillus thuringiensis subsp. jegathesan. Appl. Environ. Microbiol. 63:4449-4455.
9. Schnepf, E., N. Crickmore, J. VanRie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:775-806.
10. Wirth, M. C., A. Delécluse, and W. E. Walton. 2001. Lack of cross-resistance to Cry19A from Bacillus thuringiensis subsp. jegathesan in Culex quinquefasciatus (Diptera: Culicidae) resistant to Cry toxins from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 67:1956-1958.
11. Wu, S.-J., C. N. Koller, D. L. Miller, L. S. Bauer, and D. H. Dean. 2000. Enhanced toxicity of Bacillus thuringiensis Cry3A δ-endotoxin in coleopterans by mutagenesis in a receptor binding loop. FEBS Lett. 473:227-232.