A combined in vitro transposition - In vivo recombination mutagenesis method to knock out genes in Escherichia coli

American Biotechnology Laboratory

Volumn 22, Issue 5, 2004, Pages 8-11

  Marincs, Ferenc ^a   McDowall, Kenneth J ^a   Stockley, Peter G ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

EID: 29244454543     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Berg DE, Schmandt MA, Lowe JB. Specificity of transposon Tn5 insertion. Genetics 1983; 105:813-28.
2. Greene RC, Smith AA. Insertion mutagenesis of the metJBLF gene cluster of Escherichia coli K-12: evidence for a metBL operon. J Bacteriol 1984; 159:767-9.
3. Treat ML, Weaver ML, Emmett MR, Johnson JR. Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcription unit. Mol Gen Genet 1984; 193:370-5.
4. Goryshin IY, Reznikoff WS. Tn5 in vitro transposition. J Biol Chem 1998; 273:7367-74.
5. www.epicentre.com/guide_to_transposomics.asp.
6. Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci 2000; 97:6640-5.
7. Murphy KC, Campellone KG, Poteete AR. PCR-mediated gene replacement in Escherichia coli. Gene 2000; 246:321-30.
8. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL. An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci 2000; 97:5978-83.