Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR

Theriogenology

Volumn 65, Issue 2, 2006, Pages 288-298

  Iwanami, Yoshihito ^a   Kobayashi, Toshihiro ^a   Kato, Megumi ^b   Hirabayashi, Masumi ^b,c   Hochi, Shinichi ^a  

^a SHINSHU UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

^c GRADUATE UNIVERSITY FOR ADVANCED STUDIES   (Japan)

Author keywords

Flow cytometry; Male germ cells; Microinsemination; RT PCR; Spermatogenesis

Indexed keywords

NONHISTONE PROTEIN; NUCLEAR PROTEIN; PRIMER DNA; PROTAMINE; PROTAMINE P2; SPERMATID TRANSITION PROTEINS; TNP2 PROTEIN, RAT;

ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL DIFFERENTIATION; CHEMISTRY; CLASSIFICATION; COCULTURE; COMPARATIVE STUDY; CYTOLOGY; FEMALE; FERTILIZATION IN VITRO; FLOW CYTOMETRY; GROWTH, DEVELOPMENT AND AGING; INTRACYTOPLASMIC SPERM INJECTION; MALE; METHODOLOGY; NEWBORN; OOCYTE; PHYSIOLOGY; PLOIDY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERTOLI CELL; SPERMATID; SPERMATOGONIUM; WISTAR RAT;

ANIMALS; ANIMALS, NEWBORN; CELL DIFFERENTIATION; CELLS, CULTURED; CHROMOSOMAL PROTEINS, NON-HISTONE; COCULTURE TECHNIQUES; DNA PRIMERS; FEMALE; FERTILIZATION IN VITRO; FLOW CYTOMETRY; MALE; NUCLEAR PROTEINS; OOCYTES; PLOIDIES; PROTAMINES; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SERTOLI CELLS; SPERM INJECTIONS, INTRACYTOPLASMIC; SPERMATIDS; SPERMATOGONIA;

EID: 29244445548     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2005.04.025     Document Type: Article

References (31)

1. M.K. Skinner Cell-cell interactions in the testis Endocr Rev 12 1991 45 77
2. A.L. Kierszenbaum Mammalian spermatogenesis in vivo and in vitro: a partnership of spermatogenic and somatic cell lineages Endocr Rev 15 1994 116 134
3. M.D. Griswold Interactions between germ cells and Sertoli cells in the testis Biol Reprod 52 1995 211 216
4. M.C. Hofmann, S. Narisawa, R.A. Hess, and J.L. Millan Immortalization of germ cells and somatic testicular cells using the SV40 large T-antigen Exp Cell Res 201 1992 417 435
5. M.C. Hofmann, R.A. Hess, E. Goldberg, and J.L. Millan Immortalized germ cells undergo meiosis in vitro Proc Natl Acad Sci USA 91 1994 5533 5537
6. L.-X. Feng, Y. Chen, L. Dettin, R.A. Reijo Pera, J.C. Herr, and E. Goldberg Generation and in vitro differentiation of a spermatogonial cell line Science 297 2002 392 395
7. N. Geijsen, M. Horoschak, K. Kim, J. Gribnau, K. Eggan, and G.Q. Daley Derivation of embryonic germ cells and male gametes from embryonic stem cells Nature 427 2004 148 154
8. J. Marh, L.L. Tres, Y. Yamazaki, R. Yanagimachi, and A.L. Kierszenbaum Mouse round spermatids developed in vitro from pre-existing spermatocytes can produce normal offspring by nuclear injection into in vivo developed mature oocytes Biol Reprod 69 2003 169 176
9. J. Tesarik, M. Bahceci, C. Ozcan, E. Greco, and C. Mendoza Restoration of fertility by in-vitro spermatogenesis Lancet 353 1999 555 556
10. Rat Genome Sequencing Project Consortium Genome sequence of the Brown Norway rat yields insights into mammalian evolution Nature 428 2004 493 521
11. Q. Zhou, J.P. Renard, G.L. Friec, V. Brochard, N. Beaujean, and Y. Cherifi Generation of fertile cloned rats by regulating oocyte activation Science 302 2003 1179
12. P. Vecino, J.A. Uranga, A. Alonso, and J. Arechaga Immortalization of type A spermatogonia using the SV40 Large T Antigen Int J Dev Biol 45 S1 2001 161 162 [abstract]
13. A. Ogura, J. Matsuda, T. Asano, O. Suzuki, and R. Yanagimachi Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring J Assist Reprod Genet 13 1996 431 434
14. D. Hue, D. Staub, M.H. Perrard-Sapori, M. Weiss, J.C. Nicolle, and M. Vigier Meiotic differentiation of germinal cells in three-week cultures of whole cell population from rat seminiferous tubules Biol Reprod 59 1998 379 387
15. M. Hirabayashi, M. Kato, T. Aoto, M. Ueda, and S. Hochi Rescue of infertile transgenic rat lines by intracytoplasmic injection of cryopreserved round spermatids Mol Reprod Dev 62 2002 295 299
16. M. Kato, A. Ishikawa, S. Hochi, and M. Hirabayashi Effect of activation regimens for rat oocytes on full-term development after round spermatid injection Contemp Top Lab Anim Sci 43/2 2004 13 15
17. K. Miyoshi, T. Kono, and K. Niwa Stage-dependent development of rat 1-cell embryos in a chemically defined medium after fertilization in vivo and in vitro Biol Reprod 56 1997 180 185
18. N.B. Hecht Regulation of "haploid expressed genes" in male germ cells J Reprod Fertil 88 1990 679 693
19. C. Marret, O. Avallet, M.H. Perrard-Sapori, and P. Durand Localization and quantitative expression of mRNAs encoding the testis-specific histone TH2B, the phosphoprotein p19, the transition proteins 1 and 2 during pubertal development and throughout the spermatogenic cycle of the rat Mol Reprod Dev 51 1998 22 35
20. D. Bunick, R. Balhorn, L.H. Stanker, and N.B. Hecht Expression of the rat protamine 2 gene is suppressed at the level of transcription and translation Exp Cell Res 188 1990 147 152
21. Y. Iwanami, M. Kobayashi, M. Kato, M. Hirabayashi, and S. Hochi An attempt at inducing differentiation into round spermatids of rat spermatogonia co-cultured with Sertoli cells Reprod Fertil Dev 17 2005 308 [abstract]
22. J. Grima, L.J. Zhu, and C.Y. Cheng Testin is tightly associated with testicular cell membrane upon its secretion by Sertoli cells whose steady-state mRNA level in the testis correlates with the turnover of and integrity of inter-testicular cell junctions J Biol Chem 272 1997 6499 6509
23. C. Mauduit, S. Hamamah, and M. Benahmed Stem cell factor/c-kit system in spermatogenesis Hum Reprod Update 5 1999 535 545
24. F. Izadyar, K. Den Ouden, L.B. Creemers, G. Posthuma, M. Parvinen, and D.G. De Rooij Proliferation and differentiation of bovine type A spermatogonia during long-term culture Biol Reprod 68 2003 272 281
25. D.R. Lee, M.T. Kaproth, and J.E. Parks In vitro production of haploid germ cells from fresh or frozen-thawed testicular cells of neonatal bulls Biol Reprod 65 2001 873 878
26. C. Boitani, M.G. Politi, and T. Menna Spermatogonial cell proliferation in organ culture of immature rat testis Biol Reprod 48 1993 761 767
27. N. Cremades, R. Bernabeu, A. Barros, and M. Sousa In-vitro maturation of round spermatids using co-culture on Vero cells Hum Reprod 14 1999 1287 1293
28. N. Cremades, M. Sousa, R. Bernabeu, and A. Barros Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids Hum Reprod 16 2001 1938 1944
29. M. Sousa, N. Cremades, C. Alves, J. Silva, and A. Barros Developmental potential of human spermatogenic cells co-cultured with Sertoli cells Hum Reprod 17 2002 161 172
30. A. Krivokharchenko, E. Popova, I. Zaitseva, L. Vil'ianovich, D. Ganten, and M. Bader Development of parthenogenetic rat embryos Biol Reprod 68 2002 829 836
31. M. Vigier, M. Weiss, M.H. Perrard, M. Godet, and P. Durand The effects of FSH and of testosterone on the completion of meiosis and the very early steps of spermiogenesis of the rat: an in vitro study J Mol Endocrinol 33 2004 729 742