Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor

FEBS Journal

Volumn 272, Issue 24, 2005, Pages 6344-6353

  Teh, Evelyn M ^a   Hewitt, Jeff ^a   Ung, Karen C ^a   Griffiths, Tanya A M ^a   Nguyen, Vinh ^a   Briggs, Sara K ^b   Mason, Anne B ^b   MacGillivray, Ross T A ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF VERMONT   (United States)

Author keywords

Epitope mapping; Monoclonal antibody; Transferrin C lobe; Transferrin transferrin receptor interaction

Indexed keywords

EPITOPE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; TRANSFERRIN; TRANSFERRIN RECEPTOR;

ARTICLE; COMPUTER MODEL; ESCHERICHIA COLI; IMMUNOBLOTTING; MOUSE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN LOCALIZATION; SWINE;

ANTIBODIES, MONOCLONAL; BINDING SITES; COMPUTER SIMULATION; EPITOPE MAPPING; EPITOPES; HELA CELLS; HUMANS; PROTEIN BINDING; RECEPTORS, TRANSFERRIN; SPECIES SPECIFICITY; TRANSFERRIN;

ESCHERICHIA COLI; SUS SCROFA;

EID: 29144536128     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.05028.x     Document Type: Article

References (31)

1. MacGillivray RTA & Mason AB (2002) Transferons. In Molecular and Cellular Iron Transport (Templeton, DM, ed.), pp. 41-69. Marcel Dekker Inc., New York, NY.
2. Anderson BF, Baker HM, Dodson EJ, Norris GE, Rumball SV, Waters JM & Baker EN (1987) Structure of human lactoferrin at 3.2-A resolution. Proc Natl Acad Sci USA 84, 1769-1773.
3. Anderson BF, Baker HM, Norris GE, Rumball SV & Baker EN (1990) Apolactoferrin structure demonstrates ligand-induced conformational change in transferrins. Nature 344, 784-787.
4. Bailey S, Evans RW, Garratt RC, Gorinsky B, Hasnain S, Horsburgh C, Jhoti H, Lindley PF, Mydin A, Sarra R & Watson JL (1988) Molecular structure of serum transferrin at 3.3-A resolution. Biochemistry 27, 5804-5812.
5. Baker EN & Lindley PF (1992) New perspectives on the structure and function of transferrins. J Inorg Biochem 47, 147-160.
6. Aisen P, Enns C & Wessling-Resnick M (2001) Chemistry and biology of eukaryotic iron metabolism. Int J Biochem Cell Biol 33, 940-959.
7. Enns CA (2002) The transferrin receptor. In Molecular and Cellular Iron Transport (Templeton, DM, ed.), pp. 71-94. Marcel and Dekker Inc., New York, NY.
8. Turkewitz AP, Amatruda JF, Borhani D, Harrison SC & Schwartz AL (1988) A high yield purification of the human transferrin receptor and properties of its major extracellular fragment. J Biol Chem 263, 8318-8325.
9. Lawrence CM, Ray S, Babyonyshev M, Galluser R, Borhani DW & Harrison SC (1999) Crystal structure of the ectodomain of human transferrin receptor. Science 286, 779-782.
10. Lebron JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN & Bjorkman PJ (1998) Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Cell 93, 111-123.
11. Bennett MJ, Lebron JA & Bjorkman PJ (2000) Crystal structure of the hereditary haemochromatosis protein HFE complexed with transferrin receptor. Nature 403, 46-53.
12. Buchegger F, Trowbridge IS, Liu LF, White S & Collawn JF (1996) Functional analysis of human/chicken transferrin receptor chimeras indicates that the carboxy-terminal region is important for ligand binding. Eur J Biochem 235, 9-17.
13. Dubljevic V, Sali A & Coding JW (1999) A conserved RGD (Arg-Gly-Asp) motif in the transferrin receptor is required for binding to transferrin. Biochem J 341, 11-14.
14. West AP Jr, Bennett MJ, Sellers VM, Andrews NC, Enns CA & Bjorkman PJ (2000) Comparison of the interactions of transferrin receptor and transferrin receptor 2 with transferrin and the hereditary hemochromatosis protein HFE. J Biol Chem 275, 38135-38138.
15. Mason AB, Tam BM, Woodworth RC, Oliver RW, Green BN, Lin LN, Brandts JF, Savage KJ, Lineback JA & MacGillivray RT (1997) Receptor recognition sites reside in both lobes of human serum transferrin. Biochem J 326, 77-85.
16. Zak O, Trinder D & Aisen P (1994) Primary receptor-recognition site of human transferrin is in the C-terminal lobe. J Biol Chem 269, 7110-7114.
17. Zak O & Aisen P (2002) A new method for obtaining human transferrin C-lobe in the native conformation: preparation and properties. Biochemistry 41, 1647-1653.
18. Jeffrey PD, Bewley MC, MacGillivray RT, Mason AB, Woodworth RC & Baker EN (1998) Ligand-induced conformational change in transferrins: crystal structure of the open form of the N-terminal half-molecule of human transferrin. Biochemistry 37, 13978-13986.
19. Bali PK & Aisen P (1991) Receptor-modulated iron release from transferrin: differential effects on N- and C-terminal sites. Biochemistry 30, 9947-9952.
20. Bali PK, Zak O & Aisen P (1991) A new role for the transferrin receptor in the release of iron from transferrin. Biochemistry 30, 324-328.
21. Sipe DM & Murphy RF (1991) Binding to cellular receptors results in increased iron release from transferrin at mildly acidic pH. J Biol Chem 266, 8002-8007.
22. Cheng Y, Zak O, Aisen P, Harrison SC & Walz T (2004) Structure of the human transferrin receptor-transferrin complex. Cell 116, 565-576.
23. Liu R, Guan JQ, Zak O, Aisen P & Chance MR (2003) Structural reorganization of the transferrin C-lobe and transferrin receptor upon complex formation: the C-lobe binds to the receptor helical domain. Biochemistry 42, 12447-12454.
24. Mason AB & Woodworth RC (1991) Monoclonal antibodies to the amino- and carboxyl-terminal domains of human transferrin. Hybridoma 10, 611-623.
25. Mason AB, Miller MK, Funk WD, Banfield DK, Savage KJ, Oliver RW, Green BN, MacGillivray RT & Woodworth RC (1993) Expression of glycosylated and nonglycosylated human transferrin in mammalian cells. Characterization of the recombinant proteins with comparison to three commercially available transferrins. Biochemistry 32, 5472-5479.
26. Ikeda RA, Bowman BH, Yang F & Lokey LK (1992) Production of human serum transferrin in Escherichia coli. Gene 117, 265-269.
27. Steinlein LM & Ikeda RA (1993) Production of N-terminal and C-terminal human serum transferrin in Escherichia coli. Enzyme Microb Technol 15, 193-199.
28. Hoefkens P, de Smit MH, de Jeu-Jaspars NM, Huijskes-Heins MI, de Jong G & van Eijk HG (1996) Isolation, renaturation and partial characterization of recombinant human transferrin and its half molecules from Escherichia coli. Int J Biochem Cell Biol 28, 975-982.
29. Prasad L, Sharma S, Vandonselaar M, Quail JW, Lee JS, Waygood EB, Wilson KS, Dauter Z & Delbaere LT (1993) Evaluation of mutagenesis for epitope mapping. Structure of an antibody-protein antigen complex. J Biol Chem 268, 10705-10708.
30. Zuccola HJ (1993) PhD Thesis, Georgia Institute of Technology, Atlanta.
31. Hall DR, Hadden JM, Leonard GA, Bailey S, Neu M, Winn M & Lindley PF (2002) The crystal and molecular structures of diferric porcine and rabbit serum transferrins at resolutions of 2.15 and 2.60 A, respectively. Acta Crystallogr D Biol Crystallogr 58, 70-80.