Sequence transfers between variable regions in a mouse antibody transgene can occur by gene conversion

Journal of Immunology

Volumn 175, Issue 12, 2005, Pages 8133-8137

  D'Avirro, Nicole ^a   Truong, David ^a   Xu, Bo ^a   Selsing, Erik ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AB V GENE; ANIMAL CELL; ARTICLE; B LYMPHOCYTE DIFFERENTIATION; CONTROLLED STUDY; DNA RECOMBINATION; GENE; GENE CONSTRUCT; GENE CONVERSION; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENE TRANSFER; GENETIC RECOMBINATION; GENETIC VARIABILITY; IMMUNOGLOBULIN VARIABLE REGION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SOMATIC HYPERCONVERSION; SPECIES DIFFERENTIATION; SPECIES IDENTIFICATION; TRANSGENE;

EID: 29144490088     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: 10.4049/jimmunol.175.12.8133     Document Type: Article

References (36)

1. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302: 575-581.
2. Alt, F. W., T. K. Blackwell, and G. D. Yancopoulos. 1987. Development of the primary antibody repertoire. Science 238: 1079-1087.
3. Storb, U. 1998. Progress in understanding the mechanism and consequences of somatic hypermutation. Immunol. Rev. 162: 5-11.
4. Reynaud, C. A., V. Anquez, H. Grimal, and J. C. Weill. 1987. A hyperconversion mechanism generates the chicken light chain preimmune repertoire. Cell 48: 379-388.
5. Thompson, C. B., and P. E. Neiman. 1987. Somatic diversification of the chicken immunoglobulin light chain gene is limited to the rearranged variable gene segment. Cell 48: 369-378.
6. H allotypes: implications for the generation of antibody diversity. Cell 60: 963-970.
7. Parng, C. L., S. Hansal, R. A. Goldsby, and B. A. Osborne. 1996. Gene conversion contributes to Ig light chain diversity in cattle. J. Immunol. 157: 5478-5486.
8. Arakawa, H., S. Furusawa. S. Ekino, and H. Yamagishi. 1996. Immunoglobulin gene hyperconversion ongoing in chicken splenic germinal centers. EMBO J. 15: 2540-2546.
9. Arakawa, H., K. Kuma, M. Yasuda, S. Ekino, A. Shimizu, and H. Yamagishi. 2002. Effect of environmental antigens on the Ig diversification and the selection of productive V-J joints in the bursa. J. Immunol. 169: 818-828.
10. Arakawa, H., K. Kuma, M. Yasuda, S. Furusawa, S. Ekino, and H. Yamagishi. 1998. Oligoclonal development of B cells bearing discrete Ig chains in chicken single germinal centers. J. Immunol. 160: 4232-4241.
11. Kong, Q., L. Zhao, S. Subbaiah, and N. Maizels. 1998. A λ 3′ enhancer drives active and untemplated somatic hypermutation of a λ 1 transgene. J. Immunol. 161: 294-301.
12. Chien, N. C., R. R. Pollock, C. Desaymard, and M. D. Scharff. 1988. Point mutations cause the somatic diversification of IgM and IgG2a antiphosphorylcholine antibodies. J. Exp. Med. 167: 954-973.
13. H gene segment encodes the immune response to phosphrylcholine: somatic mutation is correlated with the class of the antibody. Cell 25: 59-66.
14. Ford, J. E., M. G. McHeyzer-Williams, and M. R. Lieber. 1994. Analysis of individual immunoglobulin λ light chain genes amplified from single cells is inconsistent with variable region gene conversion in germinal-center B cell somatic mutation. Eur. J. Immunol 24: 1816-1822.
15. Bernard, O., N. Hozumi, and S. Tonegawa. 1978. Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell 15: 1133-1144.
16. Wysocki, L. J., and M. L. Gefter. 1989. Gene conversion and the generation of antibody diversity. Annu. Rev. Biochem. 58: 509-531.
17. Muramatsu, M., K. Kinoshita, S. Fagarasan, S. Yamada, Y. Shinkai, and T. Honjo. 2000. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 102: 553-563.
18. Arakawa, H., J. Hauschild, and J. M. Buerstedde. 2002. Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion. Science 295: 1301-1306.
19. Chaudhuri, J., M. Tian, C. Khuong, K. Chua, E. Pinaud, and F. W. Alt. 2003. Transcription-targeted DNA deamination by the AID antibody diversification enzyme. Nature 422: 726-730.
20. Bransteitter, R., P. Pham, M. D. Scharff, and M. F. Goodman. 2003. Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase. Proc. Natl. Acad. Sci. USA 100: 4102-4107.
21. Pham, P., R. Bransteitter, J. Petruska, and M. F. Goodman. 2003. Processive AID-catalysed cytosine deamination on single-stranded DNA simulates somatic hypermutation. Nature 424: 103-107.
22. Dickerson, S. K., E. Market, E. Besmer, and F. N. Papavasiliou. 2003. AID mediates hypermutation by deaminating single stranded DNA. J. Exp. Med. 197: 1291-1296.
23. Ramiro, A. R., P. Stavropoulos, M. Jankovic, and M. C. Nussenzweig. 2003. Transcription enhances AID-mediated cytidine deamination by exposing single-stranded DNA on the nontemplate strand. Nat. Immunol. 4: 452-456.
24. Sale. J. E., D. M. Calandrini, M. Takata, S. Takeda, and M. S. Neuberger. 2001. Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation. Nature 412: 921-926.
25. Xu, B., and E. Selsing. 1994. Analysis of sequence transfers resembling gene conversion in a mouse antibody transgene. Science 265: 1590-1593.
26. Selsing, E., B. Xu, and D. Sigurdardottir. 1996. Gene conversion and homologous recombination in murine B cells. Semin. Immunol. 8: 151-158.
27. -/- mutant of the chicken DT40 cell line. Cell 89: 185-193.
28. D'Avirro, N., D. Truong, M. Luong, R. Kanaar, and E. Selsing. 2002. Gene conversion-like sequence transfers between transgenic antibody V genes are independent of RAD54. J. Immunol. 169: 3069-3075.
29. Tsai, H. F., N. D'Avirro, and E. Selsing. 2002. Gene conversion-like sequence transfers in a mouse antibody transgene: antigen selection allows sensitive detection of V region interactions based on homology. Int. Immunol. 14: 55-64.
30. Durdik, J., R. M. Gerstein, S. Rath, P. F. Robbins, A. Nisonoff, and E. Selsing. 1989. Isotype switching by a microinjected μ immunoglobulin heavy chain gene in transgenic mice. Proc. Natl. Acad. Sci. USA 86: 2346-2350.
31. Gerstein, R. M., W. N. Frankel, C.-L. Hsieh, J. M. Durdik, S. Rath, J. M. Coffin, A. Nisonoff, and E. Selsing. 1990. Isotype switching of an immunoglobulin heavy chain transgene occurs by DNA recombination between different chromosomes. Cell 63: 537-548.
32. Carlson, L. M., W. T. McCormack, C. E. Postema, E. H. Humphries, and C. B. Thompson. 1990. Templated insertions in the rearranged chicken IgL V gene segment arise by intrachromosomal gene conversion. Genes Dev. 4: 536-547.
33. Cumano, A., and K. Rajewsky. 1986. Clonal recruitment and somatic mutation in the generation of immunologic memory to the hapten NP. EMBO J. 5: 2459-2468.
34. David, V., N. L. Folk, and N. Maizels. 1992. Germ line variable regions that match hypermutated sequences in genes encoding murine anti-hapten antibodies. Genetics 132: 799-811.
35. Ruckerl, F., and J. Bachl. 2004. Activation-induced cytidine deaminase fails to induce a mutator phenotype in the human pre-B cell line Nalm-6. Eur. J. Immunol. 35: 290-298.
36. Muramatsu, M., V. S. Sankaranand, S. Anant, M. Sugai, K. Kinoshita, N. O. Davidson, and T. Honjo. 1999. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 274: 18470-18476.