Gel purified LipL32: A prospective antigen for detection of leptospirosis

Journal of Postgraduate Medicine

Volumn 51, Issue 3, 2005, Pages 164-168

  Tahiliani, P ^a   Mohan Kumar, M ^a   Chandu, D ^a   Kumar, A ^a   Nagaraj, C ^b   Nandi, D ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b Government of India   (India)

Author keywords

Gel elution; Human IgG ELISA; Leptospira; LipL32

Indexed keywords

IMMUNOGLOBULIN G; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN;

AGGLUTINATION TEST; ANTIGEN DETECTION; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BLOOD SAMPLING; DIAGNOSTIC APPROACH ROUTE; DIAGNOSTIC TEST; DIFFERENTIAL DIAGNOSIS; EARLY DIAGNOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; GEL; LEPTOSPIRA INTERROGANS; LEPTOSPIROSIS; POLYCLONAL ACTIVATION; PROTEIN PURIFICATION; PROTEIN SECRETION;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; LEPTOSPIRA; LEPTOSPIROSIS; LIPOPROTEINS;

EID: 28844472541     PISSN: 00223859     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, et al, Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 2003;3:757-71.
2. Vinetz JM. Leptospirosis. Curr Opin Infect Dis 2001;14:527-38.
3. Rathinam SR. Ocular leptospirosis. Curr Opin Ophthalmol 2002;13:381-6.
4. Saengjaruk P, Chaicumpa W, Watt G, Bunyaraksyotin G, Wuthiekanun V, Tapchaisri P, et al. Diagnosis of human leptospirosis by monoclonal antibody-based antigen detection in urine. J Clin Microbiol 2002;40:480-9.
5. nd edn. Cold Spring Harbor (NY): Spring Harbor Laboratory; 1989.
6. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
7. Cooper HM, Paterson Y. Production of Antibodies. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, editors, Current protocols in Immunology. New York: John Wiley and sons Inc; 1995. p. 2.4.1-2.4.9.
8. Haake DA, Chao G, Zuerner RL, Barnett JK, Barnett D, Mazel M, et al. The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection. Infect Immun 2000;68:2276-85.
9. Lechtzier V, Hutoran M, Levy T, Kotler M, Brenner T, Steinitz M. Sodium dodecyl sulphate-treated proteins as ligands in ELISA. J Immunol Methods 2000;270:19-26.
10. Dey S, Mohan CM, Kumar TM, Ramadass P, Nainar AM, Nachimuthu K. Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis. Vet Microbiol 2004;103:99-106.
11. Flannery B, Costa D, Carvalho FP, Guerreiro H, Matsunaga J, Da Silva ED, et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. J Clin Microbiol 2001;39:3303-10.
12. Zuerner RL, Knudtson W, Bolin CA, Trueba G. Characterization of outer membrane and secreted proteins of Leptospira interrogans serovar pomona. Microb Pathog 2001;10:311-22.
13. Guerreiro H, Croda J, Flannery B, Mazel M, Matsunaga J, Galvao RM. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infect Immun 2001;69:4958-68.