Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers

BMC Biochemistry

Volumn 6, Issue , 2005, Pages

  Adam, Petra ^a   Gütlich, Markus ^a   Oschkinat, Hartmut ^b   Bacher, Adelbert ^a   Eisenreich, Wolfgang ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID DERIVATIVE; ASPARAGINE; ASPARTIC ACID; CARBOHYDRATE; CARBON 13; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; NITROGEN 15; NUCLEOSIDE; PENTOSE PHOSPHATE; PHENYLALANINE; PROTEIN; RIBONUCLEOSIDE DERIVATIVE; TRACER;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL METABOLISM; INSECT CELL; ISOTOPE LABELING; LEPIDOPTERA; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; QUANTITATIVE ANALYSIS; RECYCLING; TRANSAMINATION;

ANIMALS; CARBON ISOTOPES; CELLS, CULTURED; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; SPODOPTERA;

ANIMALIA; EUKARYOTA; INSECTA; LEPIDOPTERA; SPODOPTERA FRUGIPERDA;

EID: 28844452104     PISSN: 14712091     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-6-24     Document Type: Article

References (24)

1. Luckow VA, Summers MD: High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors. Virology 1989, 170:31-39.
2. Brüggert M, Rehm T, Shanker S, Georgescu J, Holak TA: A novel medium for expression of proteins selectively labeled with 15N-amino acids in Spodoptera frugiperda (Sf9) insect cells. J Biomol NMR 2003, 25:335-348.
3. Strauss A, Bitsch F, Cutting B, Fendrich G, Graff P, Liebetanz J, Zurinin M, Jahnke W: Amino-acid-type selective isotope labeling of proteins expressed in Baculovirus-infected insect cells useful for NMR studies. J Biomol NMR 2003, 26:367-372.
4. Weiss SA, Godwin GP, Gorfien SF, Whitford WG: Serum-free media. Bioprocess Technology 1993, 17:179-194.
5. Blomberg F, Maurer W, Rüterjans H: Nitrogen-15 nuclear magnetic resonance investigations on amino acids. Proc Natl Acad Sci USA 1976, 73:1409-1413.
6. Rabenstein DL, Mariappan SVS: Determination of 15N isotope effects on the acid-base equilibria of amino groups in amino acids by carbon-13 NMR. J Org Chem 1993, 58:4487-4489.
7. Leimer KR, Rice RH, Gehrke CW: Complete mass spectra of N-trifluoroacetyl-n-butyl esters of amino acids. J Chromatogr 1977, 141:121-144.
8. Eisenreich W, Strauß G, Werz U, Bacher A, Fuchs G: Retrobiosynthetic analysis of carbon fixation in the phototrophic eubacterium Chloroflexus aurantiacus. Eur J Biochem 1993, 215:619-632.
9. 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur J Biochem 1995, 232:433-438.
10. 13C tracer experiments and metabolite balancing for metabolic flux analysis: comparing two approaches. Biotechnol Bioeng 1998, 58:254-257.
11. 13C NMR flux ratio analysis of Escherichia coli central carbon metabolism in microaerobic bioprocesses. J Am Chem Soc 1999, 121:1407-1408.
12. Park SM, Klapa MI, Sinskey AJ, Stephanopoulos G: Metabolite and isotopomer balancing in the analysis of metabolic cycles: II. Applications. Biotechnol Bioeng 1999, 62:392-401.
13. Bacher A, Rieder C, Eichinger D, Arigoni D, Fuchs G, Eisenreich W: Elucidation of biosynthetic pathways and metabolic flux patterns via retrobiosynthetic NMR analysis. FEMS Microbiol Rev 1999, 22:567-598.
14. Kruger NJ, Ratcliffe RG, Roscher A: Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling. Phytochem Rev 2003, 2:17-30.
15. Glawischnig E, Gierl A, Tomas A, Bacher A, Eisenreich W: Starch biosynthesis and intermediary metabolism in maize kernels. Quantitative analysis of metabolite flux by NMR. Plant Physiol 2003, 130:1717-1727.
16. Eisenreich W, Ettenhuber C, Laupitz R, Theus C, Bacher A: Isotopolog perturbation techniques for metabolic networks. Metabolic recycling of nutritional glucose in Drosophila melanogaster. Proc Natl Acad Sci USA 2004, 101:6764-6769.
17. Strauss A, Bitsch F, Fendrich G, Graff P, Knecht R, Meyhack B, Jahnke W: Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells. J Biomol NMR 2005, 31:343-349.
18. Silva FJ, Bel Y, Botella LM, Cotton RG, Ferre J: Immunological detection of phenylalanine hydroxylase protein in Drosophila melanogaster. Biochem J 1992, 287:85-89.
19. Bel Y, Wiesner P, Kayser H: Candidate target mechanisms of the growth inhibitor cyromazine: studies of phenylalanine hydroxylase, puparial amino acids, and dihydrofolate reductase in dipteran insects. Arch Insect Biochem Physiol 2000, 45:69-78.
20. Ruiz-Vázques P, Silva FJ: Aberrant splicing of the Drosophila melanogaster phenylalanine hydroxylase pre-mRNA caused by the insertion of a B104/roo transposable element in the Henna locus. Insect Biochem Mol Biol 1999, 29:311-318.
21. 15N NMR. J Biotechnol 2000, 78:23-27.
22. Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959, 37:911-917.
23. Eisenreich W, Schwarzkopf B, Bacher A: Biosynthesis of nucleotids, flavins, and deazaflavins in Methanobacterium thermoautotrophicum. J Biol Chem 1991, 226:9622-9631.
24. Eisenreich W, Bacher A: Elucidation of biosynthetic pathways by retrodictive/predictive comparison of isotopomer patterns determined by NMR spectroscopy. Volume 22. Edited by: Setlow JK. Genetic Engineering, Principles and Methods. Kluwer Academic/ Plenum Publisher, New York; 2000:121-153.