Determination of a ginseng saponin metabolite, IH901, in rat plasma by liquid chromatography-tandem mass spectrometry

Journal of Liquid Chromatography and Related Technologies

Volumn 28, Issue 20, 2005, Pages 3205-3216

  Min, Kyo Jeoung ^a   Youn, Bok Chung ^a   Pung, Sok Lee ^a   Chang, Soo Kim ^a   Nam, Hee Kim ^a   Dong, Ju Son ^a   Jin, Tae Hong ^a   Moon, Dong Cheul ^a  

^a Chungbuk National University   (South Korea)

Author keywords

20 O D Glucopyranosyl 20(S) protopanaxadiol; Compound K; IH901; LC MS MS; Rat plasma

Indexed keywords

ACETONITRILE; IONIZATION; MASS SPECTROMETRY; METABOLISM; PLASMA APPLICATIONS; PRECIPITATION (CHEMICAL); PROTEINS;

COMPOUND K; GINSENG SAPONIN METABOLITE; RAT PLASMAS;

LIQUID CHROMATOGRAPHY;

20 O BETA DEXTRO GLUCOPYRANOSYL 20 PROTOPANAXADIOL; GINSENG SAPONIN; PANAXADIOL; PREDNISOLONE; PROTOPANAXADIOL 20 GLUCOSIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY; ELUTION; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; NONHUMAN; QUANTITATIVE ANALYSIS; RAT; TANDEM MASS SPECTROMETRY;

ANIMALIA; PANAX GINSENG;

EID: 28744445284     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070500330885     Document Type: Article

References (20)

1. Attele, A.S.; Wu, J.A.; Yuan, C.S. Ginseng pharmacology multiple constituents and multiple actions. Biochem. Pharmacol. 1999, 58, 1685-1693.
2. Huang, K.C. The Pharmacology of Chinese Herbs', CRC Press: Boca Raton, FL, 1999.
3. Karikura, M.; Miyase, T.; Tanizawa, H.; Takino, Y.; Taniyama, T.; Hayashi, T. Studies on the absorption, distribution, excretion, and metabolism of ginseng saponins V. Chem. Pharm. Bull. 1990, 38 (10), 2859-2861.
4. Hasegawa, H.; Sung, J.H.; Huh, J.D. Role of human intgestinal Prevotella oris in hydrolyzing ginseng saponins. Planta Med. 1997, 63, 436-440.
5. Lee, B.H.; Lee, S.J.; Hui, J.H.; Lee, S.Y.; Sung, J.H.; Huh, J.D.; Moon, J.K. In vitro antigenotoxic activity of ginseng saponin metabolites formed by intestinal bacteria. Planta Med 1998, 64, 500-503.
6. Hasegawa, H.; Matsumiya, S.; Murakami, C.; Kurokawa, T.; Kasai, R.; Ishibashi, S.; Yamasaki, K. Interaction of ginseng extract, ginseng separated fractions, and some triterpenoid sapomin with glucose transporters in sheep erythrocytes. Planta Med 1994, 60, 153-157.
7. Hasegawa, H.; Sung, J.H.; Huh, J.D. Ginseng intestinal bacterial metabolite IH-901 as a new anti-metastatic agent. Arch. Pharm. Res. 1997, 20, 539-544.
8. Lim, W.K.; Sung, J.H.; Seong, Y.H. General pharmacology of IH-901. J. Applied Pharmacol. 2003, 11, 183-189.
9. Corthout, J.; Naessens, T.; Apers, S.; Vlietinck, A.J. Quantitative determination of ginsenosides from panax ginseng roots and ginseng preparations by thin layer chromatography-densitometry. J. Pharmaceut. Biomed. Anal. 1999, 21, 187-192.
10. Xu, Q.F.; Fang, X.L.; Chen, D.F. Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats. J. Ethnopharmacol. 2003, 84, 187-192.
11. Park, M.K.; Park, J.H.; Han, S.B.; Shin, Y.G.; Park, I.H. High performance liquid chromatographic analysis of ginseng saponins using evaporative light scattering detection. J. Chromatogr. A. 1996, 736, 77-81.
12. Shangguan, D.; Han, H.; Zhao, R.; Zhao, Y.; Xiong, S.; Liu, G. New method for high performance liquid chromatographic separation and fluorescence detectiom of ginsenosides. J. Chromatogr. A 2001, 910, 367-372.
13. Cui, J.F.; Garle, M.; Lund, E.; Björkhem, I.; Eneroth, P. Analysis of ginsenosides by chromatography and mass spectrometry. Release of 20 S-protopanaxadiol and 20 S-protopanaxatriol for quantitation. Anal. Biochem. 1993, 210, 411-417 .
14. Akao, T.; Kanaoka, M.; Kobashi, K. Apperarance of compound K, a major metabolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral administration - Measurement of compound K by enzyme immunoassay. Chem. Pharm. Bull. 1998, 21 (3), 245-249.
15. Mona, A.T.; Ute, B.; Michael, K.; Mario, W.; Manfred, S.Z. Degradation of ginsenosides in humans after oral administration. Drug metabolism and disposition. 2003, 31, 1065-1071.
16. Bae, E.A.; Choo, M.K.; Park, E.K.; Park, S.Y.; Shin, H.Y.; Kim, D.H. Metabolism of ginsenoside Rc by human intestinal bacteria and its related antiallergic activity. Biol. Pharm. Bull. 2002, 25 (6), 743-747.
17. (R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activity. Biol. Chem. Bull. 2002, 25 (1), 58-63.
18. Miao, X.S.; Metcalfe, C.D.; Hao, C.; March, R.E. Electrospray ionization mass spectrometry of ginsenosides. J Mass Spectrom. 2002, 37, 495-506.
19. Wang, X.; Sakuma, T.; Asafu-Adjaye, E.; Shiu, G.K. Determination of ginsenosides in plant extracts from Panax ginseng and Panax quinquefolius L. by LC/MS/MS. Anal Chem. 1999, 71 (8), 1579-1584.
20. Cai, Z.; Qian, T.; Wong, R.N.S.; Jiang, Z.H. Liquid chromatography- electrospray ionization mass spectrometry for metabolism and pharmacokinetic studies of ginsenoside Rg3. Anal. Chimica Acta. 2003, 492, 283-293.