Enhancement of mouse sperm motility by the PI3-kinase inhibitor LY294002 does not result in toxic effects on preimplantation embryo development

Human Reproduction

Volumn 20, Issue 12, 2005, Pages 3500-3504

  Luconi, Michaela ^a   Torcia, Simona ^b   Grillo, Domenico ^b   Fiorenza, Maria Teresa ^b   Forti, Gianni ^a   Mangia, Franco ^b   Baldi, Elisabetta ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Embryo; Phosphatidylinositol 3 kinase; Sperm motility

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; PHOSPHATIDYLINOSITOL 3 KINASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG MECHANISM; DRUG STRUCTURE; EMBRYO; EMBRYO DEVELOPMENT; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; FERTILIZATION; IN VITRO STUDY; MALE; MOUSE; NIDATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPERMATOZOON CAPACITATION; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; TOXICITY TESTING; WESTERN BLOTTING;

ANIMALIA;

EID: 28544442352     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/dei248     Document Type: Article

References (17)

1. Andersen AN, Gianaroli L, Felberbaum R, de Mouzon J and Nygren KG; The European IVF-monitoring programme (EIM), European Society of Human Reproduction and Embryology (ESHRE). (2005) Assisted reproductive technology in Europe, 2001. Results generated from European registers by ESHRE. Hum Reprod 20,1158-1176.
2. Bonduelle M, Wennerholm UB, Loft A, Tarlatzis BC, Peters C, Henriet S, Mau C, Victorin-Cederquist A, Van Steirteghem A, Balaska A et al. (2005) A multi-centre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Hum Reprod 20,413-419.
3. Burdon T, Smith A and Savatier P (2002) Signalling, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol 12,432-438.
4. Carr DW, Fujita A, Stentz CL, Liberty GA, Olson GE and Narumiya S (2001) Identification of sperm specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J Biol Chem 276,17332-17338.
5. du Plessis SS, Franken DR, Baldi E and Luconi M (2004) Phosphatidylinositol 3-kinase inhibition enhances human sperm motility and sperm-zona pellucida binding. Int J Androl 27,19-26.
6. Hoshino Y, Yokoo M, Yoshida N, Sasada H, Matsumoto H and Sato E (2004) Phosphatidylinositol 3kinase and Akt participate in the FSH-induced meiotic maturation of mouse oocytes. Mol Reprod Dev 69,77-86.
7. Liguori L, de Lamirande E, Minelli A and Gagnon C (2005) Various protein kinases regulate human sperm acrosome reaction and the associated phosphorylation of Tyr residues and of the Thr-Glu-Tyr motif. Mol Hum Reprod 11,211-221.
8. Luconi M and Baldi E (2003) How do sperm swim? Molecular mechanisms underlying sperm motility. Cell Mol Biol (Noisy-le-grand) 49,357-369.
9. Luconi M, Marra F, Gandini L, Filimberti E, Lenzi A, Forti G and Baldi E (2001) Phosphatidylinositol 3-kinase inhibition enhances human sperm motility. Hum Reprod 16,1931-1937.
10. Luconi M, Carloni V, Marra F, Ferruzzi P, Forti G and Baldi E (2004) Increased phosphorylation of AKAP by inhibition of phosphatidylinositol 3-kinase enhances human sperm motility through tail recruitment of protein kinase A. J Cell Sci 117,1235-1246.
11. Luconi M, Porazzi I, Ferruzzi P, Marchiani S, Forti G and Baldi E (2005) Tyrosine phosphorylation of the a kinase anchoring protein 3 (AKAP3) and soluble adenylate cyclase are involved in the increase of human sperm motility by bicarbonate. Biol Reprod 72,22-32.
12. Nagy A, Gertsenstein M, Vintersten K and Behringer R (eds) (2003) Manipulating the Mouse Embryo, 3rd edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Nauc V, De Lamirande E, Leclerc P and Gagnon C (2004) Inhibitors of phosphoinositide 3-kinase, LY294002 and wortmannin, affect sperm capacitation and associated phosphorylation of proteins differently: Ca2+dependent divergences. J Androl 25,573-585.
14. Quill TA, Sugden SA, Rossi KL, Doolittle LK, Hammer RE and Garbers DL (2003) Hyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Natl Acad Sci USA 100, 14869-14874.
15. Quinn P, Kerin JF and Warnes GM (1985) Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 44,493-498.
16. Vijayaraghavan S, Liberty GA, Mohan J, Winfrey VP, Olson GE and Carr DW (1999) Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein. Mol Endocrinol 13,705-717.
17. Whittingham, DG (1971) Culture of mouse ova. J Reprod Fertil Suppl 14,7-21.