The desaturase from Bacillus subtilis, a promising tool for the selective olefination of phospholipids

Journal of Biotechnology

Volumn 121, Issue 1, 2006, Pages 49-53

  Bonamore, Alessandra ^a   MacOne, Alberto ^a   Colotti, Gianni ^b   Matarese, Rosa Marina ^a   Boffi, Alberto ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b CNR   (Italy)

Author keywords

Bacillus subtilis; Bacterial desaturases; Cyclopropane fatty acid synthase; Fatty acid methyl esters; Gas chromatography mass spectrometry; Unsaturated fatty acids

Indexed keywords

ENZYME KINETICS; ESCHERICHIA COLI; ESTERS; FATTY ACIDS; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; OLEFINS; PHOSPHOLIPIDS;

BACILLUS SUBTILIS; BACTERIAL DESATURASES; CYCLOPROPANE FATTY ACID SYNTHASE; FATTY ACID METHYL ESTERS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; UNSATURATED FATTY ACIDS;

ENZYMES;

5 PALMITOLEIC ACID; DELTA5 DESATURASE; EFEDRENIC ACID; ENZYME; OXYGEN; PHOSPHOLIPID DERIVATIVE; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; LIPID COMPOSITION; NONHUMAN; OLEFINATION; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; ESCHERICHIA COLI; FATTY ACID DESATURASES; OXIDATION-REDUCTION; PHOSPHOLIPIDS; RECOMBINANT PROTEINS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 28444443442     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.07.008     Document Type: Article

References (14)

1. P.S. Aguilar, J.E. Cronan Jr., and D. De Mendoza A Bacillus subtilis gene induced by cold shock encode a membrane phospholipid desaturase J. Bacteriol. 180 8 1998 2194 2200
2. P.S. Aguilar, A.M. Hernandez Arriaga, L. Cybulsky, A. Erazo, and D. De Mendoza Molecular basis of thermosensing: a two-component signal transduction thermometer in Bacillus subtilis EMBO J. 20 7 2000 1681 1691
3. 5 desaturase J. Bacteriol. 185 10 2003 3228 3231
4. B. Behrouzian, and P.H. Buist Fatty acid desaturation: variation on an oxidative theme Curr. Opin. Chem. Biol. 6 5 2002 577 582
5. H.R. Buser, H. Arn, P. Guerin, and S. Rauscher Determination of double bond position in mono-unsaturated acetates by mass spectrometry of dimethyl disulfide adducts Anal. Chem. 55 1 1983 818 822
6. A.R. Diaz, M.C. Mansilla, A.J. Vila, and D. De Mendoza Membrane topology of the acyl-lipid desaturase from Bacillus subtilis J. Biol. Chem. 277 50 2002 48099 48106
7. T. Lewis, P.D. Nichols, and T.A. McMeekin Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs J. Microbiol. Meth. 43 2 2000 107 116
8. D.A. Los, and N. Murata Structure and expression of fatty acid desaturases Biochim. Biophys. Acta 1394 1 1998 1403 1415
9. T.O. Olagbemiro, M.A. Birkett, A.J. Mordue, and J.A. Pickett Production of 5R,6S-6-acetoxy-5-hexadecanolide, the mosquito oviposition pheromone from the seed oil of the summer cypress plant Kochia scoparia (Chenopodiaceae) J. Agric. Food Chem. 47 8 1999 3411 3415
10. A. Sutherland, K. Auclair, and J.C. Vederas Recent advances in the biosynthetic studies of lovastatin Curr. Opin. Drug Discov. Devel. 4 2 2001 229 236
11. F.R. Taylor, D.W. Grogan, and J.E. Cronan Jr. Cyclopropane fatty acid synthase from Escherichia coli Meth. Enzymol. 71 1981 133 139
12. D.R. Tocher, M.J. Leaver, and P.A. Hodgson Recent advances in the biochemistry and molecular biology of fatty acyl desaturases Prog. Lipid Res. 37 2-3 1998 73 117
13. R.L. Wolff, and W.W. Christie Structures, practical sources gymnosperm seeds, gas-liquid chromatographic data equivalent chain lengths, and mass spectrometric characteristics of all-cis Δ5-olefmic acids Eur. J. Lipid Sci. Technol. 104 1 2002 234 244
14. R.L. Wolff, W.W. Christie, A. Pedrono, A.M. Marpeau, N. Tsevegsuren, K. Aitzetmuller, and F.D. Gunstone Δ5-Olefinic acids in the seed lipids from four Ephedra species and their distribution between the and in the positions of triacylglicerols characteristics common to Coniferophytes and Cycadophytes Lipids 34 8 1998 855 864