The Bacillus subtilis acyl lipid desaturase is a Δ5 desaturase

Journal of Bacteriology

Volumn 185, Issue 10, 2003, Pages 3228-3231

  Altabe, Silvia G ^a   Aguilar, Pablo ^a,c   Caballero, Gerardo M ^b   De Mendozal, Diego ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b Secretaria de Turismo y Deporte   (Argentina)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL LIPID DESATURASE; BACTERIAL ENZYME; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; COVALENT BOND; ENZYME ACTIVITY; ENZYME ANALYSIS; FATTY ACID SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

BACILLUS SUBTILIS; ESCHERICHIA COLI; FATTY ACID DESATURASES; FATTY ACIDS, UNSATURATED; MASS SPECTROMETRY; TEMPERATURE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0037966887     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.10.3228-3231.2003     Document Type: Article

References (18)

1. Aguilar, P. S., J. E. Cronan, Jr., and D. de Mendoza. 1998. A Bacillus subtilis gene induced by cold shock encodes a membrane phospholipid desaturase. J. Bacteriol. 180:2194-2200.
2. Aguilar, P. S., P. Lopez, and D. de Mendoza. 1999. Transcriptional control of the low-temperature-inducible des gene encoding the Δ5 desaturase of Bacillus subtilis. J. Bacteriol. 181:7028-7033.
3. Aguilar, P. S., A. M. Hernandez-Arriaga, L. E. Cybulsky, A. C. Erazo, and D. de Mendoza. 2001. Molecular basis of thermosensing: a two component transduction thermometer in Bacillus subtilis. EMBO J. 20:1-11.
4. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 31:911-917.
5. Christie, W. 1989. Gas chromatography and lipids. The Oily Press, Ayr, Scotland.
6. de Mendoza, D., G. Schujman, and P. S. Aguilar. 2002. Biosynthesis and function of membrane lipids, p. 43-55. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and its relatives: from genes to cells. ASM Press, Washington D.C.
7. Diaz, A. R., M. C. Mansilla, A. J. Vila, and D. de Mendoza. 2002. Membrane topology of the acyl-lipid desaturase from Bacillus subtilis. J. Biol. Chem. 277:48099-48106.
8. Harvey, D. J. 1982. Picolinyl esters as derivatives for the structural determination of long chain branched and unsaturated fatty acids. Biomed. Mass Spectrom. 9:33-38.
9. Kaneda, T. 1991. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function and taxonomic significance. Microbiol. Rev. 55:288-302.
10. Klein, W., M. H. W. Weber, and M. A. Marahiel. 1999. Cold shock response of Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures. J. Bacteriol. 181:5341-5349.
11. Lindquist, Y., W. Huang, G. Schneider, and J. Shanklin. 1996. Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins. EMBO J. 15:4081-4092.
12. Meesapydsuk, D., D. W. Reed, C. H. Savile, P. Buist, S. Ambrose, and P. S. Covello. 2000. Characterization of the regiochemistry and cryptoregiochemistry of a Caenorhabditis elegans fatty acid desaturase (FAT-1) expressed in Saccharomyces cerevisiae. Biochemistry 39:11948-11954.
13. Murata, N., and H. Wada. 1995. Acyl-lipid desaturases and their importance in the tolerance and acclimation to cold of cyanobacteria. Biochem. J. 308:1-8.
14. Shanklin, J., E. Whittle, and B. G. Fox. 1994. Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33:12787-12794.
15. Shanklin, J., and E. B. Cahoon. 1998. Desaturation and related modifications of fatty acids. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49:611-641.
16. Spizizen, J. 1958. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. USA 44:1072-1078.
17. Weber, M. H. W., W. Klein, L. Muller, U. M. Niess, and M. A. Marahiel. 2001. Role of the Bacillus subtilis fatty acid desaturase in membrane adaptation during cold shock. Mol. Microbiol. 39:1321-1329.
18. Yamamoto, K., A. Shibahara, T. Nakayama, and G. Kajimoto. 1991. Determination of double-bond positions in methylene-interrupted dienoic fatty acids by GC-MS as their dimethyl disulfide adducts. Chem. Phys. Lipids 60:39-50.