Production of human caseinomacropeptide in recombinant Saccharomyces cerevisiae and Pichia pastoris

Journal of Industrial Microbiology and Biotechnology

Volumn 32, Issue 9, 2005, Pages 402-408

  Kim, Yu Jin ^a   Oh, You Kwan ^a   Kang, Whankoo ^b   Lee, Eun Yeol ^c   Park, Sunghoon ^a  

^a Pusan National University   (South Korea)

^b Hannam University   (South Korea)

^c Kyungsung University   (South Korea)

Author keywords

Caseinomacropeptide; Fed batch; Promoter; Recombinant yeast; Secretion

Indexed keywords

AMINO ACID; CASEIN; GALACTOSE; PEPTIDE DERIVATIVE; POLYPEPTIDE;

ARTICLE; BIOLOGICAL ACTIVITY; BIOREACTOR; CARBOXY TERMINAL SEQUENCE; FED BATCH REACTOR; NONHUMAN; PEPTIDE SYNTHESIS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

BIOMASS; BIOREACTORS; CASEINS; FERMENTATION; FUNGAL PROTEINS; GALACTOSE; GENES, SYNTHETIC; GENETIC VECTORS; HUMANS; PEPTIDE FRAGMENTS; PICHIA; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

MAMMALIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 28344448370     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-005-0010-2     Document Type: Article

References (17)

1. El-Salam MH-Abd, El-Shibiny S, Buchheim W (1995) Characteristics and potential uses of the casein macropeptide. Int Dairy J 6:327-341
2. Pihlanto A, Korhonen H (2003) Bioactive peptide and proteins. Advances in food and nutrition research. Academic, London
3. Totsuka M, Katakura Y, Shimizu M, Kumagak I, Miura K, Kaminogawa S (1990) Expression and secretion of bovine β-Lactoglobulin in Saccharomyces cerevisiae. Agic Biol Chem 54:3111-3116
4. Choi BK, Jimènez-Flores R (2001) Expression and purification of glycosylated bovine βcasein (L70S/P71S) in Pichia pastoris. J Agric Food Chem 49:1761-1766
5. Paramasivam M, Saravanan K, Uma K, Sharma S, Singh TP, Srinivasan A (2002) Expression, purification, and characterization of equine lactoferrin in Pichia pastoris. Protein Expr Purif 26:28-34
6. Mercier JC, Chobert JM (1976) Comparative study of the amino acid sequences of the caseinomacropeptide from seven species. FEBS 72:208-214
7. Sudbery PE (1996) The expression of recombinant proteins in yeasts. Curr Opin Biotechnol 7:517-524
8. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66
9. Gellissen G (2000) Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 54:741-750
10. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
11. Kozak M (1987) An analysis of 5′- noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 15:8125-8148
12. Chung BH, Seo DJ, Nam SW, Na JG (1997) Optimization of feeding strategy for overproduction of human lipocortin-I in Saccharomyces cerevisiae controlled by the GAL10 promoter. J Ferment Bioeng 84:466-470
13. Chung BH, Seo DJ, Nam SW (1999) High-level secretory production of recombinant human lipocortin-1 by Saccharomyces cerevisiae. Process Biochem 35:97-101
14. Chung BH, Kim BM, Rhee SK, Park YH, Nam SW (1995) Effect of galactose feeding strategy on heterologous human lipocortin-I production in the fed-batch culture of Saccharomyces cerevisiae controlled by the GAL 10 promoter. J Microbiol Biotechnol 5:224-228
15. Bradford MM (1976) A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248-254
16. Mylin LM, Hofmann, KJ, Schultz LD, Hopper JE (1990) Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast. Methods Enzymol 185:297-308
17. Sohn JH, Lee SK, Choi ES, Rhee SK (1991) Gene expression and Secretion of the anticoagulant hirudin in Saccharomyces cerevisiae. J Micorbiol Biotechnol 1:266-273