Immobilization of fuculose-1-phosphate aldolase from Escherichia coli to glyoxal-agarose gels by multipoint covalent attachment

Biocatalysis and Biotransformation

Volumn 23, Issue 3-4, 2005, Pages 241-250

  Suau, Trinitat ^a   Calveras, Jordi ^b   Clapés, Pere ^b   Benaiges, Maria Dolors ^a   Álvaro, Gregorio ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b DEPARTMENT OF ENVIRONMENTAL CHEMISTRY   (Spain)

Author keywords

Agarose gel; Aldol condensation; Covalent attachment; Enzyme immobilization; Fuculose 1 phosphate aldolase; Non conventional media

Indexed keywords

ALDEHYDES; CONDENSATION; DERIVATIVES; EMULSIONS; ESCHERICHIA COLI; GELS; PH EFFECTS; PHOSPHATES; POLYSACCHARIDES; SOLUBILITY; SYNTHESIS (CHEMICAL); THERMAL EFFECTS;

AGAROSE GEL; ALDOL CONDENSATION; COVALENT ATTACHMENT; FUCULOSE-1-PHOSPHATE ALDOLASE; NON-CONVENTIONAL MEDIA;

ENZYME IMMOBILIZATION;

AGAROSE; DIHYDROXYACETONE PHOSPHATE; FRUCTOSE BISPHOSPHATE ALDOLASE; PROPIONALDEHYDE; RECOMBINANT ENZYME;

ALDOL REACTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; COVALENT BOND; EMULSION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PH; TEMPERATURE DEPENDENCE;

ESCHERICHIA COLI;

EID: 28244493707     PISSN: 10242422     EISSN: 10292446     Source Type: Journal    
DOI: 10.1080/10242420500208720     Document Type: Article

References (22)

1. Álvaro G, Fernandez-Laftiente R, Blanco RM, Guisán JM. 1990. Immobilization-stabilization of penicillin G acylase from E. coli. Appl Biochem and Biotech 26:181-195.
2. Bednarski MD. 1991. Applications of enzymic aldol reactions in organic synthesis. Appl Biocatal 1:87-116.
3. Blanco RM, Calvete JJ, Guisán JM. 1989. Immobilizationstabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment. Enzyme Microb Technol 11:353-359.
4. Budde CL, Khmelnitsky YL. 1999. Aldolase stability in the presence of organic solvents. Biotechnol Lett 21:77-80.
5. Durany O, Caminal. G, de Mas C, López-Santin J. 2004a. Studies on the expression of recombinant fuculose-1-phosphate aldolase in E. coli. Process Biochem 39:1677-1684.
6. Durany O, de Mas C, López-Santín J. 2004b. Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli. Process Biochem 40:707-716.
7. Effenberger F, Straub A. 1987. A novel convenient preparation of dihydroxyacetone phosphate and its use in enzymatic aldol reactions. Tetrahedron Lett 28:1641-1644.
8. Espelt L, Parella T, Bujons J, Solans C, Joglar J, Delgado A, Clapes P. 2003a. Stereoselective aldol additions catalysed by dihydroxyacetone phosphate dependent aldolases in emulsion systems: preparation and structural characterization of linear and cyclic aminopolyols from aminoaldehydes. Chem-Eur J 9:4887-4899.
9. Espelt L, Clapés P, Esquena J, Manich A, Solans C. 2003b. Enzymatic Carbon (Carbon bond formation in water-in-oil highly concentrated emulsions (Gel Emulsions) Langmuir 19:1337-1346.
10. Espelt L, Bujons J, Patella T, Calveras J, Joglar J, Delgado A, Clapés P. 2005. Aldol additions of dihydroxyacetone phosphate to N-Cbz-amino aldehydes catalyzed by 1-fuculose-1-phosphate aldolase in emulsion systems: inversion of stereoselectivity as a function of the acceptor aldehyde. Chem Eur J 11:1392-1401.
11. Fessner W, Schneider A, Eyrisch O, Sinerius G, Badia J. 1993. 6-Deoxy-L-lyxo- and 6-deoxy-L-arabino-hexulose 1-phosphates. Enzymic synthesis by antagonistic metabolic pathways. Tetrehedron Asymm 41:781-790.
12. Guisán JM. 1988. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb Technol 10:375-382.
13. Guisán JM, Fernandez-Lafuente R, Rodriguez V, Bastida A, Blanco RM, Álvaro G. 1993. Enzyme stabilization by multipoint covalent attachment to activated pre-existing supports. In: Van den Tweel WJJ, Harder A, Buitelaar RM. editors. Stability and Stabilization of Enzymes. Amsterdam: Elsevier. 47:55-62.
14. Joerger AC, Gosse C, Fessner W-D, Schulz GE. 2000. Catalytic action of Fuculose 1-phosphate aldolase (Class II) as derived from structure-directed mutagenesis. Biochemistry 39:6033-6041.
15. Jung SH, Jeong JH, Miller P, Wong CH. 1994. An efficient multigram-scale preparation of dihydroxyacetone phosphate. J Org Chem 59:7182-7184.
16. Morís-varas F, Qian XH, Wong CH. 1996. Enzymatic/chemical synthesis and biological evaluation of seven-membered iminocyclitols. CJ Am Chem Soc 118:7647-7652.
17. Rojo J, Morales JC, Penades S. 2002. Carbohydrate-carbohydrate interactions in biological model systems. Topics in Current Chemistry 218:45-92.
18. Sobolov SB, Bartoszko-Malik A, Oeschger TR, Montelbano MM. 1994. Crosslinked enzyme crystals of fructose diphosphate aldolase: development as a biocatalyst for synthesis. Tetrahedron Lett 35:7751-7754.
19. Takayama S, McGarvey GJ, Wong CH. 1997. Microbial aldolases and transketolases: new biocatalytic approaches to simple and complex sugars. Annu Rev Microbiol 51:285-310.
20. Tijoe TT, Van der Wiel JP, Stickkelman RM, Straathof AJ, Van Rantwijk F. 1985. High-performance liquid chromatographic determination of glucose 1-phosphate and glucuronic acid 1-phosphate. J Chromatogr 330:412-414.
21. Toone EJ, Simon ES, Bednarski MD, Whitesides GM. 1989. Enzyme-catalysed synthesis of carbohydrates. Tetrahedron. 45:5365-5377.
22. Zagalak B, Frey P, Karabatsos GL, Abeles R. 1996. The stereochemistry of the conversion of D and L 1,2-propanediols to propionaldehyde. J Biol Chem 241:3028-3035.