Molecular cloning and characterization of rat LC3A and LC3B - Two novel markers of autophagosome

Biochemical and Biophysical Research Communications

Volumn 339, Issue 1, 2006, Pages 437-442

  Wu, Jiaxue ^a   Dang, Yongjun ^a   Su, Wei ^a   Liu, Chao ^a   Ma, Haijie ^a   Shan, Yuxi ^a   Pei, Yuan ^a   Wan, Bo ^a   Guo, Jinhu ^a   Yu, Long ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

Autophagy; LC3A; LC3B; Post translational modification; Rat LC3; Subcellular localization

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; AUTOPHAGY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; MOLECULAR CLONING; MUTATION; NUCLEOTIDE SEQUENCE; PHAGOSOME; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; YEAST;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; AUTOPHAGY; CELL LINE; CLONING, MOLECULAR; HUMANS; INTRACELLULAR MEMBRANES; MICROTUBULE-ASSOCIATED PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; ORGAN SPECIFICITY; PHAGOSOMES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RATS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 28144431882     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.10.211     Document Type: Article

References (22)

1. D.J. Klionsky, and S.D. Emr Autophagy as a regulated pathway of cellular degradation Science 290 2000 1717 1721
2. I. Tanida, T. Ueno, and E. Kominami LC3 conjugation system in mammalian autophagy Int. J. Biochem. Cell Biol. 36 2004 2503 2518
3. S.A. Kuznetsov, and V.I. Gelfand 18 kDa microtubule-associated protein: identification as a new light chain (LC-3) of microtubule-associated protein 1 (MAP-1) FEBS Lett. 212 1987 145 148
4. T.A. Schoenfeld, L. McKerracher, R. Obar, and R.B. Vallee MAP 1A and MAP 1B are structurally related microtubule associated proteins with distinct developmental patterns in the CNS J. Neurosci. 9 1989 1712 1730
5. S.S. Mann, and J.A. Hammarback Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B J. Biol. Chem. 269 1994 11492 11497
6. T. Lang, E. Schaeffeler, D. Bernreuther, M. Bredschneider, D.H. Wolf, and M. Thumm Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole EMBO J. 17 1998 3597 3607
7. Y. Kabeya, N. Mizushima, T. Ueno, A. Yamamoto, T. Kirisako, T. Noda, E. Kominami, Y. Ohsumi, and T. Yoshimori LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19 2000 5720 5728
8. J. Hemelaar, V.S. Lelyveld, B.M. Kessler, and H.L. Ploegh A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L J. Biol. Chem. 278 2003 51841 51850
9. I. Tanida, Y.S. Sou, J. Ezaki, N. Minematsu-Ikeguchi, T. Ueno, and E. Kominami HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates J. Biol. Chem. 279 2004 36268 36276
10. Y. Kabeya, N. Mizushima, A. Yamamoto, S. Oshitani-Okamoto, Y. Ohsumi, and T. Yoshimori LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation J. Cell Sci. 117 2004 2805 2812
11. I. Tanida, E.M. Tanida, T. Ueno, and E. Kominami The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3 J. Biol. Chem. 276 2001 1701 1706
12. I. Tanida, E.M. Tanida, M. Komatsu, T. Ueno, and E. Kominami Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p J. Biol. Chem. 277 2002 13739 13744
13. Y. Ichimura, T. Kirisako, T. Takao, Y. Satomi, Y. Shimonishi, N. Ishihara, N. Mizushima, I. Tanida, E. Kominami, M. Ohsumi, T. Noda, and Y. Ohsumi A ubiquitin-like system mediates protein lipidation Nature 408 2000 488 492
14. T. Kouno, M. Mizuguchi, I. Tanida, T. Ueno, T. Kanematsu, Y. Mori, H. Shinoda, M. Hirata, E. Kominami, and K. Kawano Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains J. Biol. Chem. 280 2005 24610 24617
15. B. Zhou, N. Boudreau, C. Coulber, J. Hammarback, and M. Rabinovitch Microtubule-associated protein 1 light chain 3 is a fibronectin mRNA-binding protein linked to mRNA translation in lamb vascular smooth muscle cells J. Clin. Invest. 100 1997 3070 3082
16. B. Zhou, and M. Rabinovitch Microtubule involvement in translational regulation of fibronectin expression by light chain 3 of microtubule-associated protein 1 in vascular smooth muscle cells Circ. Res. 83 1998 481 489
17. S. Furuta, K. Miura, T. Copeland, W.H. Shang, A. Oshima, and T. Kamata Light chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling Oncogene 21 2002 7060 7066
18. H. He, Y. Dang, F. Dai, Z. Guo, J. Wu, X. She, Y. Pei, Y. Chen, W. Ling, C. Wu, S. Zhao, J.O. Liu, and L. Yu Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B J. Biol. Chem. 278 2003 29278 29287
19. I. Tanida, T. Ueno, and E. Kominami Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes J. Biol. Chem. 279 2004 47704 47710
20. C.I. Seidenbecher, M. Landwehr, K.H. Smalla, M. Kreutz, D.C. Dieterich, W. Zuschratter, C. Reissner, J.A. Hammarback, T.M. Bockers, E.D. Gundelfinger, and M.R. Kreutz Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins J. Mol. Biol. 336 2004 957 970
21. T. Kirisako, Y. Ichimura, H. Okada, Y. Kabeya, N. Mizushima, T. Yoshimori, M. Ohsumi, T. Takao, T. Noda, and Y. Ohsumi The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway J. Cell Biol. 151 2000 263 276
22. D.B. Munafo, and M.I. Colombo A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation J. Cell Sci. 14 2001 3619 3629