Radiation-induced DNA double-strand breaks in dependence on protein concentration and under aerobic and anaerobic conditions

Radiation Physics and Chemistry

Volumn 75, Issue 2, 2006, Pages 210-217

  Distel, L ^a   Schwotzer, G ^a   Schussler H ^b  

^a Strahlenklinik ^*  (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

DNA double strand breaks; Oxygen enhancement; Protein peroxyl radicals; Radiation protection by proteins; Radiolysis of DNA

Indexed keywords

FREE RADICALS; OXYGEN; PHOSPHATES; X RAYS;

OXYGEN ENHANCEMENT; RADIOLYSIS OF DNA;

DNA;

DOUBLE STRANDED DNA; PEROXY RADICAL; SERUM ALBUMIN;

ARTICLE; CHEMICAL REACTION; DNA CROSS LINKING; DNA STRAND BREAKAGE; ELECTROPHORESIS; PROTEIN DNA INTERACTION; RADIATION INJURY; RADIATION PROTECTION; X IRRADIATION;

EID: 27944502408     PISSN: 0969806X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.radphyschem.2005.08.018     Document Type: Article

References (21)

1. -) in aqeous solution J. Phys. Chem. Data 17 1988 513 886
2. L.V.R. Distel, and H. Schuessler The effect of serum albumin on the radiolysis of DNA studied by constant field electrophoresis and compared to alterations caused by low molecular weight OH-scavengers Int. J. Radiat. Biol. 71 1997 401 412
3. L. Distel, B. Distel, and H. Schüssler Formation of DNA double-strand breaks and DNA-protein crosslinks by irradiation of DNA in the presence of a protein Radiat. Phys. Chem. 65 2002 141 149
4. J. Du, and J.M. Gebicki Proteins are major initial cell targets of hydroxyl free radicals Intern. J. Biochem. Cell Biol. 36 2004 2334 2343
5. J.M. Gebicki Protein hydroperoxides as new reactive oxygen species Redox Report 3 1997 99 110
6. S. Gebicki, and M. Gebicki Crosslinking of DNA and proteins induced by protein hydroperoxides Biochem. J. 338 1999 629 636
7. 2 species studied by pulse radiolysis Z.Naturforsch. 38a 1983 589 590
8. 4- in wässriger Lösung I. Geschwindigkeitskonstanten der Reaktionen mit den Primärprodukten der Wasserradiolyse Int. J. Radiat. Phys. Chem. 5 1973 393 403
9. M. Ljungman, S. Nyberg, J. Nygren, M. Erikson, and G. Ahnstrom DNA-bound proteins contribute much more than soluble intracellular compounds to the intrinsic protection against radiation-induced DNA strand breaks in human cells Radiat. Res. 127 1991 171 176
10. L.K. Mee Radiation Chemistry of Biopolymers Fahartaziz M.A.J. Rodgers Radiation Chemistry, Principles and Applications 1987 VCH Publishers, Inc. New York 477 499
11. J. Nygren, M. Ljungman, and G. Ahnström Chromatin structure and radiation-induced DNA strand breaks in human cells: soluble scavengers and DNA-bound proteins offer a better protection against single- than double-strand breaks Int. J. Radiat. Biol. 68 1995 11 18
12. T. Paul, M.J. Young, I.E. Hill, and K.U. Ingold Strand cleavage of supercoiled DNA by water-soluble peroxyl radicals. The overlooked importance of peroxyl radical charge Biochemistry 39 2000 4129 4135
13. R. Roots, and S. Okada Estimation of life times and diffusion distances of radicals involved in X-ray- induced DNA strand breaks or killing of mammalian cells Radiat. Res. 64 1975 306 320
14. A. Sak, M. Stuschke, R. Wurm, and V. Budach Protection of DNA from radiation-induced double-strand breaks: influence of replication and nuclear proteins Int. J. Rad. Biol. 76 2000 749 756
15. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning. A Laboratory Manual 2nd Ed 1989 Cold Spring Harbor Press New York, NY
16. H. Schuessler, and E. Jung Protein-DNA crosslinks induced by primary and secondary radicals Int. J. Radiat. Biol. 56 1989 423 435
17. H. Schuessler, L. Distel, and R. Sieber Radiolysis of DNA in the presence of a protein studied by HPL-gel chromatography Int. J. Rad. Biol. 71 1997 543 553
18. H. Schüssler, S. Navaratnam, and L. Distel Pulse radiolysis studies on histones and serum albumin under different ionic conditions Radiat. Phys. Chem. 61 2001 123 128
19. H. Schüssler, S. Navaratnam, and L. Distel Rate constants for the reactions of DNA with hydrated electrons and with OH-radicals, Radiat Phys. Chem. 73 2005 163 168
20. C. von Sonntag The Chemical Basis of Radiation Biology 1987 Taylor and Francis London, New York, Philadelphia
21. C. von Sonntag Free-radical-induced chain scission and crosslinking of polymers in aqueous solution-an overview Radiat. Phys. Chem. 67 2003 353 359