Gene replacement in Haloarcula marismortui: Construction of a strain with two of its three chromosomal rRNA operons deleted

Extremophiles

Volumn 9, Issue 6, 2005, Pages 427-435

  Tu, Daqi ^a   Blaha, Gregor ^a   Moore, Peter B ^a   Steitz, Thomas A ^a,b  

^a YALE UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Gene replacement; Haloarcula marismortui; Ribosomal protein L22; rRNA Operon

Indexed keywords

DNA; ESCHERICHIA COLI PROTEIN; PRIMER DNA; RIBOSOME PROTEIN; RIBOSOME RNA; RNA 23S; RNA BINDING PROTEIN; RPLV PROTEIN, E COLI; SUCROSE;

ARTICLE; BIOLOGICAL MODEL; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; CHROMOSOME MAP; ELECTRON; GENE DELETION; GENETIC PROCEDURES; GENETICS; HALOARCULA MARISMORTUI; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; SOUTHERN BLOTTING; X RAY CRYSTALLOGRAPHY;

BASE SEQUENCE; BLOTTING, SOUTHERN; CHROMOSOME MAPPING; CRYSTALLOGRAPHY, X-RAY; DNA; DNA PRIMERS; ELECTRONS; ESCHERICHIA COLI PROTEINS; GENE DELETION; GENETIC TECHNIQUES; HALOARCULA MARISMORTUI; MODELS, CHEMICAL; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OPERON; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBOSOMAL PROTEINS; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 23S; RNA-BINDING PROTEINS; RRNA OPERON; SUCROSE;

HALOARCULA MARISMORTUI; HALOBACTERIUM SALINARUM;

EID: 27844590293     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-005-0459-y     Document Type: Article

References (28)

1. Arndt E (1990) Nucleotide sequence of four genes encoding ribosomal proteins from the 'S10 and spectinomycin' operon equivalent region in the archaebacterium Halobacterium marismortui. FEBS Lett 267:193-198
2. Arndt E (1992) The genes for ribosomal protein L15 and the protein equivalent to secY in the archaebacterium Haloarcula (Halobacterium) marismortui. Biochim Biophys Acta 1130:113-116
3. Arndt E, Kromer W, Hatakeyama T (1990) Organization and nucleotide sequence of a gene cluster coding for eight ribosomal proteins in the archaebacterium Halobacterium marismortui. J Biol Chem 265:3034-3039
4. Arndt E, Weigel C (1990) Nucleotide sequence of the genes encoding the L11, L1, L10 and L12 equivalent ribosomal proteins from the archaebacterium Halobacterium marismortui. Nucleic Acids Res 18:1285
5. Baliga NS, Bonneau R, Facciotti MT, Pan M, Glusman G, Deutsch EW, Shannon P, Chiu Y, Weng RS, Gan RR, Hung P, Date SV, Marcotte E, Hood L, Ng WV (2004) Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res 14:2221-2234
6. Ban N, Freeborn B, Nissen P, Penczek P, Grassucci RA, Sweet R, Frank J, Moore PB, Steitz TA (1998) A 9 Å resolution X-ray crystallographic map of the large ribosomal subunit. Cell 93:1105-1115
7. Ban N, Nissen P, Hansen J, Moore PB, Steitz TA (2000) The complete atomic structure of the large ribosomal subunit at 2.4 A resolution. Science 289:905-920
8. Chang S, Cohen SN (1979) High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111-115
9. Charlebois RL, Lam WL, Cline SW, Doolittle WF (1987) Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium. Proc Natl Acad Sci USA 84:8530-8534
10. Chittum HS, Champney WS (1994) Ribosomal protein gene sequence changes in erythromycin-resistant mutants of Escherichia coli. J Bacteriol 176:6192-6198
11. Cline SW, Doolittle WF (1987) Efficient transfection of the archaebacterium Halobacterium halobium. J Bacteriol 169:1341-1344
12. Cline SW, Doolittle WF (1992) Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons. J Bacteriol 174:1076-1080
13. Cline SW, Lam WL, Charlebois RL, Schalkwyk LC, Doolittle WF (1989) Transformation methods for halophilic archaebacteria. Can J Microbiol 35:148-152
14. Dennis PP, Ziesche S, Mylvaganam S (1998) Transcription analysis of two disparate rRNA operons in the halophilic archaeon Haloarcula marismortui. J Bacteriol 180:4804-4813
15. Dyall-Smith M (2003) The Halohandbook: protocols for halobacterial genetics. http://www.microbiol.unimelb.edu.au/staff/mds/HaloHandbook/ (accessed March 2005)
16. Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA (2002) The structures of four macrolide antibiotics bound to the large ribosomal subunit. Mol Cell 10:117-128
17. Kita T, Brown MS, Goldstein JL (1980) Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in livers of mice treated with mevinolin, a competitive inhibitor of the reductase. J Clin Invest 66:1094-1100
18. Klebe RJ, Harriss JV, Sharp ZD, Douglas MG (1983) A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene 25:333-341
19. Kraft A, Lutz C, Lingenhel A, Grobner P, Piendl W (1999) Control of ribosomal protein L1 synthesis in mesophilic and thermophilic archaea. Genetics 152:1363-1372
20. Krebs MP, Mollaaghababa R, Khorana HG (1993) Gene replacement in Halobacterium halobium and expression of bacteriorhodopsin mutants. Proc Natl Acad Sci USA 90:1987-1991
21. Lam WL, Doolittle WF (1989) Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc Natl Acad Sci USA 86:5478-5482
22. Mevarech M, Hirsch-Twizer S, Goldman S, Yakobson E, Eisenberg H, Dennis PP (1989) Isolation and characterization of the rRNA gene clusters of Halobacterium marismortui. J Bacteriol 171:3479-3485
23. Ramírez C, Köpke AKE, Yang D-C, Boeckh T, Matheson AT (1993) The structure, function and evolution of archaeal ribosomes. In: Kates M, Kushner DJ, Matheson AT (eds) The biochemistry of archaea (archaebacteria), vol 26. Elsevier Science Publishers BV, Amsterdam, The Netherlands, pp439-466
24. Scholzen T, Arndt E (1991) Organization and nucleotide sequence often ribosomal protein genes from the region equivalent to the spectinomycin operon in the archaebacterium Halobacterium marismortui. Mol Gen Genet 228:70-80
25. Stern S, Moazed D, Noller HF (1988) Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension. In: Harry F, Noller J, Moldave K (eds) Methods in enzymology, vol 164. Academic Press Inc., San Diego, pp 481-489
26. BK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance. Cell 121:257-270
27. Unge J, berg A, Al-Kharadaghi S, Nikulin A, Nikonov S, Davydova N, Nevskaya N, Garber M, Liljas A (1998) The crystal structure of ribosomal protein L22 from Thermus thermophilus: insights into the mechanism of erythromycin resistance. Structure 6:1577-1586
28. Weisblum B (1995) Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother 39:577-585