Molecular characterization of marker-free transgenic lines of indica rice that accumulate carotenoids in seed endosperm

Molecular Genetics and Genomics

Volumn 274, Issue 4, 2005, Pages 325-336

  Parkhi, V ^a,b   Rai, M ^a   Tan, J ^a   Oliva, N ^a   Rehana, S ^a   Bandyopadhyay, A ^a   Torrizo, L ^a   Ghole, V ^b   Datta, K ^a   Datta, S K ^a,c  

^a INTERNATIONAL RICE RESEARCH INSTITUTE   (Philippines)

^b UNIVERSITY OF PUNE   (India)

^c UNIVERSITY OF CALCUTTA   (India)

Author keywords

Carotene; Agrobacterium tumefaciens; Co transformation; High iron rice; HPLC

Indexed keywords

CAROTENOID;

ARTICLE; CULTIVAR; ENDOSPERM; GENETIC MARKER; METABOLIC CAPACITY; MOLECULAR GENETICS; NUTRITIONAL VALUE; PLANT GENETICS; PRIORITY JOURNAL; RHIZOBIUM; RICE; TRANSGENE; TRANSGENIC PLANT;

BETA CAROTENE; BLOTTING, SOUTHERN; CAROTENOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC TECHNIQUES; GENETIC VECTORS; GENOTYPE; LINKAGE (GENETICS); MODELS, GENETIC; ORYZA SATIVA; PEPTIDES; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RHIZOBIUM; SEEDS; SPECIES SPECIFICITY; TIME FACTORS; TRANSGENES;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; RHIZOBIUM;

EID: 27844482943     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-005-0030-7     Document Type: Article

References (38)

1. Breitler J, Meynard D, Boxtel J, Royer M, Bonnot F, Cambillau L, Guiderdoni E (2004) A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (Oryza sativa L). Transgenic Res 13:271-278
2. Cotsaftis O, Sallaud C, Breitler JC, Meynard D, Greco R, Pereira A, Guiderdoni E (2002) Transposon-mediated generation of T-DNA and maker free rice plants expressing a Bt endotoxin gene. Mol Breed 10:165-180
3. Daley M, Knauf VC, Summerfelt KR, Turner JC (1998) Cotransformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17:489-496
4. Datta SK, Peterhans A, Datta K, Potrykus I (1990) Genetically engineered fertile Indica-rice plants recovered from protoplasts. Biotechnology 8:736-740
5. Datta K, Koukolíková-Nicola Z, Baisakh N, Oliva N, Datta SK (2000) Agrobacterium-mediated engineering for sheath blight resistance of indica rice cultivars from different ecosystems. Theor Appl Genet 100:832-839
6. Datta K, Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan J, Rai M, Rehana S, Al-Babili S, Beyer P, Potrykus I, Datta S (2003a) Bioengineered ' golden' indica rice cultivar with β-carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnol J 1:81-90
7. Datta SK, Chandel G, Tu J, Baisakh N, Datta K (2003b) Engineering of Bt transgenic rice for insect pest protection. In: Metz M (ed) Bacillus thuringienesis: a cornerstone of modern agriculture (Part III). Food Products, Binghamton, pp 77-91
8. DeBlock M, DeBrouwer D (1991) 2 T-DNAs co-transformed into Brassica napus by double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theor Appl Genet 82:257-263
9. Demmig-Adams B, Adams WW (2002) Antioxidants in photosynthesis and human nutrition. Science 298:2149-2153
10. Depicker A, Herman L, Jacobs S, Schell J, van Montagu M (1985) Frequency of simultaneous transformation with different T-DNAs and their relevance to the A grobacterium-plant cell interaction. Mol Gen Genet 201:477-484
11. Ducreux LJM, Morris WL, Hedley PE, Sheperd T, Davies HV, Millam S, Taylor MA (2005) Metabolic engineering of high carotenoid potato tubers containing enhanced level of β-carotene and lutein. J Expt Bot 56:81-89
12. Edward K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
13. Hellens R, Mullineaux P, Klee H (2000) A guide to Agrobacterium binary Ti vectors. Trends Plant Sci 5:446-451
14. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271-228
15. Hoa TTC, Al-Babili S, Schaub P, Potrykus I, Beyer P (2003) Golden indica and japonica rice lines amenable to deregulation. Plant Physiol 133:161-169
16. Huang S, Gilbertson LA, Adams TH, Malloy K, Reisenbigler EK, Birr DH, Snyder MW, Zhang Q, Luethy MH (2004) Generation of marker free transgenic maize by regular two right-border Agrobacterium transformation vectors. Transgenic Res 13:451-461
17. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
18. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vector carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10:165-174
19. Krinsky NI, Landrum JT, Bone RA (2003) Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Annu Rev Nutr 23:171-201
20. Lloyd AM, Davis RW (1994) Functional expression of the yeast FLP/FRT site specific recombination system in Nicotiana tabacum. Mol Gen Genet 242:653-657
21. Lu H-J, Zhou X-R, Gong Z-X, Upadhyaya NM (2001) Generation of selectable marker free transgenic rice using double right-border vectors. Aust J Plant Physiol 28:241-248
22. Miller M, Tagliani L, Wang N, Berka B, Sidney D, Zhao Z-Y (2002) High frequency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system. Transgenic Res 11:381-396
23. Misawa N, Yamano S, Linden H, DeFelip MR, Lucas M, Ikenaga H, Sandmann G (1993) Functional expression of Erwinia uredovora carotenoid biosynthesis gene crtI in transgenic plants showing an increase of β-carotene biosynthesis activity and resistance to bleaching herbicide norflurazon. Plant J 4:833-840
24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:474-497
25. Naik PS, Chanemougasoudharam A, Paul Khurana SM, Kalloo G (2003) Genetic manipulation of carotenoid pathway in higher plants. Curr Sci 85:1423-1430
26. Odell J, Caimi P, Sauer B, Russell S (1990) Site directed recombination in the genome of transgenic tobacco. Mol Gen Genet 223:369-378
27. Poirier Y, Ventre G, Nawrath C (2000) High-frequency linkage of co-expressing T-DNA in transgenic Arabidopsis thaliana transformed by vacuum-infiltration of Agrobacterium tumefaciens. Theor Appl Genet 100:487-493
28. Rao R, Abrigo E, Rai M, Oliva N, Datta K, Datta SK (2003) Marker-free Bt transgenic rice. Rice Genet Newslett 20:51-53
29. Romer S, Fraser PD, Kiano JW, Shipton CA, Misawa N, Schuch W, Bramley P (2000) Elevation of provitamin A content of transgenic tomato plants. Nat Biotechnol 18:666-669
30. Romer S, Lubeck J, Kauder F, Steiger S, Adomat C, Sandmann G (2002) Genetic engineering of zeaxanthin-rich potato by anti-sense inactivation and co-suppression of carotenoid epoxidation. Metab Eng 4:263-272
31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory, Cold Spring Harbor
32. Schledz M, Al-Babili S, von Lintig J, Haubruck H, Rabbani S, Kleinig H, Beyer P (1996) Phytoene synthase from Narcissus pseudonarcissus: functional expression, galactolipid requirement, topological distribution in chromoplasts and induction during flowering. Arch Biochem Biophys 385:4-12
33. Tan J, Baisakh N, Oliva N, Parkhi V, Rai M, Oliva N, Torrizo L, Datta K, Datta SK (2005) The screening of rice germplasm, including those transgenic rice lines which accumulate β-carotene in their polished seeds, for their carotenoid profile. Int J Food Sci Technol 40:563-569
34. Tu J, Datta K, Oliva N, Zhang G, Xu C, Khush GS, Zhang Q, Datta SK (2003) Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation. Plant Biotechnol J 1:155-165
35. Vain P, Afolabi A, Worland B, Snape W (2003) Transgene behavior in populations of rice plants transformed using a new dual binary vector system: pGreen/pSoup. Theor Appl Genet 107:201-217
36. Vasconcelos M, Datta K, Oliva N, Khalekuzzaman M, Torrizo L, Krishnan S, Oliveira M, Goto F, Datta SK (2003) Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene. Plant Sci 64:371-378
37. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:302-305
38. Zubko E, Scutt C, Meyer P (2000) Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol 18:442-445