Chlorhexidine-induced ultrastructural alterations in oral biofilm

Microscopy Research and Technique

Volumn 68, Issue 2, 2005, Pages 85-89

  Vitkov, Ljubomir ^a,b   Hermann, A ^b   Krautgartner, W D ^a   Herrmann, M ^a   Fuchs, K ^c   Klappacher, M ^b   Hannig, M ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY OF SALZBURG   (Austria)

^c Landeskrankenhaus Voecklabruck   (Austria)

Author keywords

Adhesion; Biofilm disintegration; Biofilm matrix; Biofilm phenotype; Fimbriae

Indexed keywords

CYTOLOGY; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; RUTHENIUM COMPOUNDS; SCANNING ELECTRON MICROSCOPY;

BACTERIAL CELLS; BACTERIAL MEMBRANES; BIOFILM DISINTEGRATION; BIOFILM MATRIX; BIOFILM PHENOTYPE; CHLORHEXIDINE; EPITHELIAL CELLS; FIMBRIA; MICROSCOPIC LEVELS; MORPHOLOGICAL ALTERATION;

BIOFILMS;

CHLORHEXIDINE; OXIDE; RUTHENIUM RED; TETROXIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL MEMBRANE; BIOFILM; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; EPITHELIUM CELL; FEMALE; GINGIVA; GLYCOCALYX; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MOUTH; NORMAL HUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

BACTERIA (MICROORGANISMS);

EID: 27644530498     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jemt.20238     Document Type: Article

References (22)

1. Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. 1995. Microbial biofilms. Annu Rev Microbiol 49:711-745
2. Cramton SE, Gerke C, Schnell NF, Nichols WW, Gotz F. 1999. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 67:5427-5433.
3. Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295-298.
4. Decker EM, Weiger R, Von Ohle C, Wiech I, Brecx M. 2003a. Susceptibility of planktonic versus attached Streptococcus sanguinis cells to chlorhexidine. Clin Oral Investig 7:98-102.
5. Decker EM, Weiger R, Wiech I, Heide PE, Brecx M. 2003b. Comparison of antiadhesive and antibacterial effects of antiseptics on Streptococcus sanguinis. Eur J Oral Sci 111:144-148.
6. Donlan RM, Costerton JW. 2002. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 15:167-193.
7. Erlandsen SL, Kristich CJ, Dunny GM, Wells CL. 2004. High-resolution visualization of the microbial glycocalyx with low-voltage scanning electron microscopy: dependence on cationic dyes. J Histochem Cytochem 52:1427-1435.
8. Fassel TA, Edmiston CE, Jr. 1999. Ruthenium red and the bacterial glycocalyx. Biotech Histochem 74:194-212.
9. Froeliger EH, Fives-Taylor P. 2001. Streptococcus parasanguis fimbria-associated adhesin fap1 is required for biofilm formation. Infect Immun 69:2512-2514.
10. Hope CK, Wilson M. 2004. Analysis of the effects of chlorhexidine on oral biofilm vitality and structure based on viability profiling and an indicator of membrane integrity. Antimicrob Agents Chemother 48:1461-1468.
11. Kaplan JB, Fine DH. 2002. Biofilm dispersal of Neisseria subflava and other phylogenetically diverse oral bacteria. Appl Environ Microbiol 68:4943-4950.
12. Mah TF, O'Toole GA. 2001. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 9:34-39.
13. Plesiat P, Aires JR, Godard C, Köhler T. 1997. Use of steroids to monitor alterations in the outer membrane of Pseudomonas aeruginosa. J Bacteriol 179:7004-7010.
14. Pratten J, Barnett P, Wilson M. 1998. Composition and susceptibility to chlorhexidine of multispecies biofilms of oral bacteria. Appl Environ Microbiol 64:3515-3519.
15. Springer EL, Roth IL. 1973. The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red. J Gen Microbiol 74:21-31.
16. Suci PA, Tyler BJ. 2002. Action of chlorhexidine digluconate against yeast and filamentous forms in an early-stage Candida albicans biofilm. Antimicrob Agents Chemother 46:3522-3531.
17. Tattawasart U, Hann AC, Maillard JY, Furr JR, Russell AD. 2000. Cytological changes in chlorhexidine-resistant isolates of Pseudomonas stutzeri. J Antimicrob Chemother 45:145-152.
18. Vaahtoniemi LH. 1997. Surface ultrastructure of intact and in situ chlorhexidine-treated human buccal cells. A method for scanning electron microscopy. Acta Odontol Scand 55:277-281.
19. Vitkov L, Krautgartner WD, Hannig M, Fuchs K. 2001. Fimbria-mediated bacterial adhesion to human oral epithelium. FEMS Microbiol Lett 202:25-30.
20. Vitkov L, Hannig M, Krautgartner WD, Fuchs K. 2002. Bacterial adhesion to sulcular epithelium in periodontitis. FEMS Microbiol Lett 211:239-246.
21. Zaura-Arite E, van Marie J, ten Cate JM. 2001. Confocal microscopy study of undisturbed and chlorhexidine-treated dental biofilm. J Dent Res 80:1436-1440.
22. Zucchelli G, Pollini F, Clauser C, De Sanctis M. 2000. The effect of chlorhexidine mouthrinses on early bacterial colonization of guided tissue regeneration membranes. An in vivo study. J Periodontol 71:263-271.