Analysis of the Effects of Chlorhexidine on Oral Biofilm Vitality and Structure Based on Viability Profiling and an Indicator of Membrane Integrity

Antimicrobial Agents and Chemotherapy

Volumn 48, Issue 5, 2004, Pages 1461-1468

  Hope, C K ^a   Wilson, M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDE; CHLORHEXIDINE; CHLORHEXIDINE GLUCONATE; HYDROXYAPATITE; TOBRAMYCIN;

ARTICLE; BACTERICIDAL ACTIVITY; BIOFILM; BIOREACTOR; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; FLUORESCENCE; IMAGE ANALYSIS; MEMBRANE; NONHUMAN; PRIORITY JOURNAL;

ANTI-INFECTIVE AGENTS, LOCAL; BIOFILMS; CELL MEMBRANE; CHLORHEXIDINE; DENTAL PLAQUE; FLUORESCENT DYES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, CONFOCAL; MOUTH;

EID: 2142705750     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.48.5.1461-1468.2004     Document Type: Article

References (35)

1. Auschill, T. M., N. B. Arweiler, L. Netuschil, M. Brecx, E. Reich, A. Sculean, and N. B. Artweiler. 2001. Spatial distribution of vital and dead microorganisms in dental biofilms. Arch. Oral Biol. 46:471-476.
2. Bassler, B. L. 2002. Small talk. Cell-to-cell communication in bacteria. Cell 109:421-424.
3. Coquet, L., G. A. Junter, and T. Jouenne. 1998. Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. J. Antimicrob. Chemother. 42:755-760.
4. Fitzgerald, K. A., A. Davies, and A. D. Russell. 1989. Uptake of 14C-chlorhexidine diacetate to Escherichia coli and Pseudomonas aeruginosa and its release by azolectin. FEMS Microbiol. Lett. 51:327-332.
5. Gilbert, P., P. J. Collier, and M. R. Brown. 1990. Influence of growth rate on susceptibility to antimicrobial agents: biofilms, cell cycle, dormancy, and stringent response. Antimicrob. Agents Chemother. 34:1865-1868.
6. Gilbert, P., J. Das, and I. Foley. 1997. Biofilm susceptibility to antimicrobials. Adv. Dent. Res. 11:160-167.
7. Gulot, E., P. Georges, A. Brun, M. P. Fontaine-Aupart, M. N. Bellon-Fontaine, and R. Briandet. 2002. Heterogeneity of diffusion inside microbial biofilms determined by fluorescence correlation spectroscopy under two-photon excitation. Photochem. Photobiol. 75:570-578.
8. Hope, C. K., and M. Wilson. 2003. Cell vitality within oral biofilms, p. 269-284. In A. J. McBain, D. Allison, M. Brading, A. Rickard, J. Verran, and J. Walker (ed.), Biofilm communities: order from chaos. Bioline, Cardiff, United Kingdom.
9. Hope, C. K., D. Clements, and M. Wilson. 2002. Determining the spatial distribution of viable and nonviable bacteria in hydrated microcosm dental plaques by viability profiling. J. Appl. Microbiol. 93:448-455.
10. Hope, C. K., and M. Wilson. 2002. Comparison of the interproximal plaque removal efficacy of two powered toothbrushes using in vitro oral biofilms. Am. J. Dent. 15B:7B-11B.
11. Hope, C. K., and M. Wilson. 2003. Effects of dynamic fluid activity from an electric toothbrush on in vitro oral biofilms. J. Clin. Periodontol. 30:624-629.
12. Hope, C. K., and M. Wilson. 2003. Measuring the thickness of an outer layer of viable bacteria in an oral biofilm by viability mapping. J. Microbiol. Methods 54:403-410.
13. Jenkins, S., M. Addy, W. Wade, and R. G. Newcombe. 1994. The magnitude and duration of the effects of some mouthrinse products on salivary bacterial counts. J. Clin. Periodontol. 21:397-401.
14. Jepras, R. I., J. Carter, S. C. Pearson, F. E. Paul, and M. J. Wilkinson. 2003. Development of a robust flow cytometric assay for determining numbers of viable bacteria. Appl. Environ. Microbiol. 61:2696-2701.
15. Jones, C. G. 1997. Chlorhexidine: is it still the gold standard? Periodontology 2000 15:55-62.
16. Keijser, J. A., H. Verkade, M. F. Timmerman, and F. A. Van der Weijden. 2003. Comparison of 2 commercially available chlorhexidine mouthrinses. J. Periodontol. 74:214-218.
17. Kinniment, S. L., J. W. Wimpenny, D. Adams, and P. D. Marsh. 1996. Development of a steady-state oral microbial biofilm community using the constant-depth film fermenter. Microbiology 142:631-638.
18. Kolenbrander, P. E., R. N. Andersen, D. S. Blehert, P. G. Egland, J. S. Foster, and R. J. Palmer, Jr. 2002. Communication among oral bacteria. Microbiol. Mol. Biol. Rev. 66:486-505.
19. Mah, T. F., and G. A. O'Toole. 2001. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 9:34-39.
20. Nealson, K. H., and J. W. Hastings. 1979. Bacterial bioluminescence: its control and ecological significance. Microbiol. Rev. 43:496-518.
21. Netuschil, L., E. Reich, G. Unteregger, A. Sculean, and M. Brecx. 1998. A pilot study of confocal laser scanning microscopy for the assessment of undisturbed dental plaque vitality and topography. Arch. Oral Biol. 43:277-285.
22. O'Neill, J. F., C. K. Hope, and M. Wilson. 2002. Oral bacteria in multispecies biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg. Med. 31:86-90.
23. Paul, F., R. Jepras, D. Hynes, A. Smith, and B. Marken. 1996. Activity of common oral antiseptics against bacteria assessed using the oxonol DIBAC4(3). Purdue cytometry CD-ROM 2(4). Purdue University Cytometry Laboratories, West Lafayette, Ind.
24. Pratten, J., and M. Wilson. 1999. Antimicrobial susceptibility and composition of microcosm dental plaques supplemented with sucrose. Antimicrob. Agents Chemother. 43:1595-1599.
25. Rolla, G., H. Loe, and C. R. Schiott. 1971. Retention of chlorhexidine in the human oral cavity. Arch. Oral Biol. 16:1109-1116.
26. Roszak, D. B., and R. R. Colwell. 2003. Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51:365-379.
27. Schiott, C. R., H. Loe, S. B. Jensen, M. Kilian, R. M. Davies, and K. Glavind. 1970. The effect of chlorhexidine mouthrinses on the human oral flora. J. Periodontal Res. 5:84-89.
28. Sheen, S., and M. Addy. 2003. An in vitro evaluation of the availability of cetylpyridinium chloride and chlorhexidine in some commercially available mouthrinse products. Br. Dent. J. 194:207-210.
29. Spoering, A. L., and K. Lewis. 2001. Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials. J. Bacteriol. 183:6746-6751.
30. Stewart, P. S. 1996. Theoretical aspects of antibiotic diffusion into microbial biofilms. Antimicrob. Agents Chemother. 40:2517-2522.
31. Stewart, P. S. 2003. Diffusion in biofilms. J. Bacteriol. 185:1485-1491.
32. Thurnheer, T., R. Gmur, S. Shapiro, and B. Guggenheim. 2003. Mass transport of macromolecules within an in vitro model of supragingival plaque. Appl. Environ. Microbiol. 69:1702-1709.
33. Wilson, M. 2001. Bacterial biofilms and human disease. Sci. Prog. 84:235-254.
34. Wilson, M., T. Burns, and J. Pratten. 1996. Killing of Streptococcus sanguis in biofilms using a light-activated antimicrobial agent. J. Antimicrob. Chemother. 37:377-381.
35. Wood, S. R., J. Kirkham, P. D. Marsh, R. C. Shore, B. Nattress, and C. Robinson. 2000. Architecture of intact natural human plaque biofilms studied by confocal laser scanning microscopy. J. Dent. Res. 79:21-27.