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Volumn 127, Issue 44, 2005, Pages 15491-15505

Reactant concentrations from fluorescence correlation spectroscopy with tailored fluorescent probes. An example of local calibration-free pH measurement

Author keywords

[No Author keywords available]

Indexed keywords

CONSTRAINT THEORY; ELECTRIC EXCITATION; FLUORESCENCE; PH EFFECTS; SPECTROSCOPIC ANALYSIS; THERMODYNAMICS; TITRATION;

EID: 27644487200     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja053909w     Document Type: Article
Times cited : (21)

References (61)
  • 11
    • 0001579306 scopus 로고
    • Fluorescence Correlation Spectroscopy
    • Lakowicz, J., Ed.; Plenum Press: New York
    • Thompson, N. L. Fluorescence Correlation Spectroscopy. Topics in Fluorescence Spectroscopy, Techniques; Lakowicz, J., Ed.; Plenum Press: New York, 1991; Vol. 1, pp 337-410.
    • (1991) Topics in Fluorescence Spectroscopy, Techniques , vol.1 , pp. 337-410
    • Thompson, N.L.1
  • 13
    • 0034755570 scopus 로고    scopus 로고
    • Elson, E. L. Traffic 2001, 2, 789-796.
    • (2001) Traffic , vol.2 , pp. 789-796
    • Elson, E.L.1
  • 18
    • 0242385316 scopus 로고    scopus 로고
    • Here, we do not consider the situation where at least one reactant is immobilized such as in, for instance: Lieto, A. M.; Cush, R. C.; Thompson, N. L. Biophys. J. 2003, 85, 3294-3302.
    • (2003) Biophys. J. , vol.85 , pp. 3294-3302
    • Lieto, A.M.1    Cush, R.C.2    Thompson, N.L.3
  • 40
    • 27644492679 scopus 로고    scopus 로고
    • note
    • ω does not significantly affect G(τ) and cannot be reliably extracted from the fit. See ref 14.
  • 42
    • 27644549316 scopus 로고    scopus 로고
    • note
    • For a given relative brightness, Q, two different H̄ values give noticeably the same Ξ value; in principle, it should not be possible to extract pH-pK from Ξ without ambiguity. In fact, the constraint on the intensity of fluorescence emission should forbid the observation of the branch attached to pH values significantly departing from pK (vide infra).
  • 45
    • 0001866318 scopus 로고
    • Porter, G., Ed.; Pergamon Press: Oxford, London, Edinburgh, New York, Paris, Frankfurt
    • Eigen, M.; Kruse, W.; Maas, G.; de Mayer, L. In Progress in Reaction Kinetics: Porter, G., Ed.; Pergamon Press: Oxford, London, Edinburgh, New York, Paris, Frankfurt, 1964, Vol. 2; pp 287-318.
    • (1964) Progress in Reaction Kinetics , vol.2 , pp. 287-318
    • Eigen, M.1    Kruse, W.2    Maas, G.3    De Mayer, L.4
  • 48
    • 27644435371 scopus 로고    scopus 로고
    • note
    • Here, one here assumes that the solution is dilute enough to neglect the reaction script A signℋ + A ⇌ A + script A signℋ
  • 54
    • 27644441087 scopus 로고    scopus 로고
    • note
    • D = 1-10 where the threshold associated to the chemical reaction is at the most visible in the FCS autocorrelation curve.
  • 55
    • 27644452066 scopus 로고    scopus 로고
    • note
    • The data are provided by the Waters Company. HPLC Troubleshooting, Neue, U. D., Waters Corporation, www.waters.com.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.