Unique insertions within Plasmodium falciparum subtilisin-like protease-1 are crucial for enzyme maturation and activity

Molecular and Biochemical Parasitology

Volumn 144, Issue 2, 2005, Pages 187-197

  Jean, Létitia ^a,b   Withers Martinez, Chrislaine ^a   Hackett, Fiona ^a   Blackman, Michael J ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Autocatalytic; Insertions; Invasion; Plasmodium; Processing; Subtilisin

Indexed keywords

PROTEIN P47; PROTOZOAL PROTEIN; SUBTILISIN LIKE PROTEASE 1; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; INSECT CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN STABILITY; SUBSTITUTION REACTION;

AMINO ACID SEQUENCE; ANIMALS; DNA TRANSPOSABLE ELEMENTS; ENZYME STABILITY; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PLASMODIUM FALCIPARUM; PROTOZOAN PROTEINS; SEQUENCE ALIGNMENT; SUBTILISINS;

BACTERIA (MICROORGANISMS); INSECTA; PLASMODIUM FALCIPARUM;

EID: 27644474297     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2005.07.008     Document Type: Article

References (35)

1. M.J. Blackman Proteases involved in erythrocyte invasion by the malaria parasite: function and potential as chemotherapeutic targets Curr Drug Targets 1 2000 59 83
2. M.J. Blackman, H. Fujioka, and W.H. Stafford A subtilisin-like protein in secretory organelles of Plasmodium falciparum merozoites J Biol Chem 273 1998 23398 23409
3. F. Hackett, M. Sajid, C. Withers-Martinez, M. Grainger, and M.J. Blackman PfSUB-2: a second subtilisin-like protein in Plasmodium falciparum merozoites Mol Biochem Parasitol 103 1999 183 195
4. J.C. Barale, T. Blisnick, and H. Fujioka Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase Proc Natl Acad Sci USA 96 1999 6445 6450
5. M. Sajid, C. Withers-Martinez, and M.J. Blackman Maturation and specificity of Plasmodium falciparum subtilisin-like protease-1, a malaria merozoite subtilisin-like serine protease J Biol Chem 275 2000 631 641
6. C. Withers-Martinez, L. Jean, and M.J. Blackman Subtilisin-like proteases of the malaria parasite Mol Microbiol 53 2004 55 63
7. M.J. Blackman Proteases in host cell invasion by the malaria parasite Cell Microbiol 6 2004 893 903
8. E. Pizzi, and C. Frontali Divergence of noncoding sequences and of insertions encoding nonglobular domains at a genomic region well conserved in plasmodia J Mol Evol 50 2000 474 480
9. E. Pizzi, and C. Frontali Low-complexity regions in Plasmodium falciparum proteins Genome Res 11 2001 218 229
10. M.J. Gardner, H. Tettelin, and D.J. Carucci Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum Science 282 1998 1126 1132
11. B. Kappes, C.D. Doerig, and R. Graeser An overview of Plasmodium protein kinases Parasitol Today 15 1999 449 454
12. H.Y. Xue, and D.R. Forsdyke Low-complexity segments in Plasmodium falciparum proteins are primarily nucleic acid level adaptations Mol Biochem Parasitol 128 2003 21 32
13. L. Brocchieri Low-complexity regions in Plasmodium proteins: in search of a function Genome Res 11 2001 195 197
14. J.C. Wootton, and S. Federhen Analysis of compositionally biased regions in sequence databases Meth Enzymol 266 1996 554 571
15. D.J. Bzik, B.A. Fox, and K. Gonyer Expression of Plasmodium falciparum lactate dehydrogenase in Escherichia coli Mol Biochem Parasitol 59 1993 155 166
16. C.R. Dunn, M.J. Banfield, and J.J. Barker The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design Nat Struct Biol 3 1996 912 915
17. P.M. Farber, K. Becker, S. Muller, R.H. Schirmer, and R.M. Franklin Molecular cloning and characterization of a putative glutathione reductase gene, the PfGR2 gene, from Plasmodium falciparum Eur J Biochem 239 1996 655 661
18. G.N. Sarma, S.N. Savvides, K. Becker, M. Schirmer, R.H. Schirmer, and P.A. Karplus Glutathione reductase of the malarial parasite Plasmodium falciparum: crystal structure and inhibitor development J Mol Biol 328 2003 893 907
19. J.L. Clarke, O. Sodeinde, and P.J. Mason A unique insertion in Plasmodium berghei glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: evolutionary and functional studies Mol Biochem Parasitol 127 2003 1 8
20. C. Withers-Martinez, E.P. Carpenter, and F. Hackett PCR-based gene synthesis as an efficient approach for expression of the A + T-rich malaria genome Protein Eng 12 1999 1113 1120
21. C. Withers-Martinez, J.W. Saldanha, B. Ely, F. Hackett, T. O'Connor, and M.J. Blackman Expression of recombinant Plasmodium falciparum subtilisin-like protease-1 in insect cells. Characterization, comparison with the parasite protease, and homology modelling J Biol Chem 277 2002 26709 29698
22. M.J. Blackman, J.E. Corrie, J.C. Croney, G. Kelly, J.F. Eccleston, and D.M. Jameson Structural and biochemical characterization of a fluorogenic rhodamine-labeled malarial protease substrate Biochemistry 41 2002 12244 12252
23. T. Gallagher Subtilisin BPN′ at 1.6 A resolution: analysis for discrete disorder and comparison of crystal forms Acta Crystallogr D: Biol Crystallogr 52 1996 1125 1135
24. E.A. Davidson, and D.C. Gowda Glycobiology of Plasmodium falciparum Biochimie 83 2001 601 604
25. L. Jean, F. Hackett, S.R. Martin, and M.J. Blackman Functional characterisation of the propeptide of Plasmodium falciparum subtilisin-like protease-1 J Biol Chem 278 2003 28572 28579
26. Z. Kostova, and D.H. Wolf For whom the bell tolls: protein quality control of the endoplasmic reticulum and the ubiquitin-proteasome connection EMBO J 22 2003 2309 2317
27. O. Almog, A. Gonzalez, D. Klein, H.M. Greenblatt, S. Braun, and G. Shoham The 0.93 A crystal structure of sphericase: a calcium-loaded serine protease from Bacillus sphaericus J Mol Biol 332 2003 1071 1082
28. P.A. Alexander, B. Ruan, and P.N. Bryan Cation-dependent stability of subtilisin Biochemistry 40 2001 10634 10639
29. T. Holyoak, C.A. Kettner, G.A. Petsko, R.S. Fuller, and D. Ringe Structural basis for differences in substrate selectivity in Kex2 and furin protein convertases Biochemistry 43 2004 2412 2421
30. S. Henrich, A. Cameron, and G.P. Bourenkov The crystal structure of the proprotein processing proteinase furin explains its stringent specificity Nat Struct Biol 10 2003 520 526
31. R.J. Siezen, and J.A. Leunissen Subtilases: the superfamily of subtilisin-like serine proteases Protein Sci 6 1997 501 523
32. Y. Li, and M. Inouye Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine J Biol Chem 269 1994 4169 4174
33. S.C. Jain, U. Shinde, Y. Li, M. Inouye, and H.M. Berman The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution J Mol Biol 284 1998 137 144
34. T. Gallagher, G. Gilliland, L. Wang, and P. Bryan The prosegment-subtilisin BPN′ complex: Crystal structure of a specific 'foldase' Structure 3 1995 907 914
35. E.D. Anderson, S.S. Molloy, F. Jean, H. Fei, S. Shimamura, and G. Thomas The ordered and compartment-specific autoproteolytic removal of the furin intramolecular chaperone is required for enzyme activation J Biol Chem 277 2002 12879 12890