A direct, continuous, sensitive assay for protein disulphide-isomerase based on fluorescence self-quenching

Biochemical Journal

Volumn 391, Issue 2, 2005, Pages 351-357

  Raturi, Arun ^a   Vacratsis, Panayiotis O ^a   Seslija, Dana ^a   Lee, Lana ^a   Mutus, Bulent ^a  

^a UNIVERSITY OF WINDSOR   (Canada)

Author keywords

Denitrosation; Diabz GSSG; Disulphide exchange; Fluorescence self quenching; Insulin turbidity; Protein disulphide isomerase

Indexed keywords

ENZYMES; FLUORESCENCE; QUENCHING; REDUCTION; RNA; ULTRAVIOLET SPECTROSCOPY;

DENITROSATION; DIABZ-GSSG; DISULPHIDE EXCHANGE; FLUORESCENCE SELF-QUENCHING; INSULIN TURBIDITY; PROTEIN DISULPHIDE-ISOMERASE;

PROTEINS;

ISOMERASE; SULFUR DERIVATIVE;

ARTICLE; CHEMICAL REACTION; FLUORESCENCE; FLUOROMETRY; KINETICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; REDUCTION; SURFACE PROPERTY; TURBIDITY; ULTRAVIOLET SPECTROSCOPY;

BLOOD PLATELETS; DISULFIDES; FLUORESCENCE; GLUTATHIONE; GLUTATHIONE DISULFIDE; HUMANS; KINETICS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN DISULFIDE-ISOMERASE; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 27444447668     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050770     Document Type: Article

References (28)

1. Goldberg, R. F., Epstein, C. J. and Anfinsen, C. B. (1963) Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver. J. Biol. Chem. 238, 628-635
2. Wilkinson, B. and Gilbert, H. F. (2004) Protein disulfide isomerase. Biochim. Biophys. Acta 1699, 35-44
3. Ramachandran, N., Root, P., Jiang, X. M., Hogg, P. J. and Mutus, B. (2001) Mechanism of transfer of NO from extracellular S-nitrosothiols into the cytosol by cell-surface protein disulfide isomerase. Proc. Natl. Acad. Sci. U.S.A. 98, 9539-9544
4. Sliskovic, I., Raturi, A. and Mutus, B. (2005) Characterization of the S-denitrosation activity of protein disulfide isomerase. J. Biol. Chem. 280, 8733-8741
5. Lundstrom, J. and Holmgren, A. (1990) Protein disulfide-isomerase is a substrate for thioredoxin reductase and has thioredoxin-like activity. J. Biol. Chem. 265, 9114-9120
6. Hillson, D. A., Lambert, N. and Freedman, R. B. (1984) Formation and isomerization of disulfide bonds in proteins: protein disulfide-isomerase. Methods Enzymol. 107, 281-294
7. Lyles, M. M. and Gilbert, H. F. (1991) Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer. J. Biol. Chem. 30, 613-619
8. Jones, L. J., Upson, R. H., Haugland, R. P., Panchuk-Voloshina, N., Zhou, M. and Haugland, R. P. (1997) Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement. Anal. Biochem. 251, 144-152
9. Wendt, H., Berger, C., Baici, A., Thomas, R. M. and Bosshard, H. R. (1995) Kinetics of folding of leucine zipper domains. Biochemistry 34, 4097-4107
10. Zhuang, X., Ha, T., Kim, H. D., Centner, T., Labeit, S. and Chu, S. (2000) Fluorescence quenching: a tool for single-molecule protein-folding study. Proc. Natl. Acad. Sci. U.S.A. 97, 241-244
11. Root, P. and Mutus, B. (2003) O-aminobenzoyl-S-nitrosohomocysteine, a fluorogenic probe for cell-surface thiol determinations via a microtiter plate assay. Anal. Biochem. 320, 299-302
12. Westphal, V., Spetzler, J. C., Meldal, M., Christensen, U. and Winther, J. R. (1998) Kinetic analysis of the mechanism and specificity of protein-disulfide isomerase using fluorescence-quenched peptides. J. Biol. Chem. 273, 24992-24999
13. Christiansen, C., St Hilaire, P. M. and Winther, J. R. (2004) Fluorometric polyethyleneglycol-peptide hybrid substrates for quantitative assay of protein disulfide isomerase. Anal. Biochem. 333, 148-155
14. Pihlajaniemi, T., Helaakoski, T., Tasanen, K., Myllyla, R., Huhtala, M. L., Koivu, J. and Kivirikko, K. I. (1987) Molecular cloning of the β-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 6, 643-649
15. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
16. Bennett, I, Edwards, B., Sklar, L. and Rogelj, S. (2000) Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes. J. Immunol. 164, 4120-4129
17. Jiang, X. M., Fitzgerald, M., Grant, C. M. and Hogg, P. J. (1999) Redox control of exofacial protein thiols/disulfides by protein disulfide isomerase. J. Biol. Chem. 274, 393-399
18. Mustard, J. F., Kinlough-Rathborne, R. L. and Packham, M. A. (1989) Isolation ot human platelets from plasma by centrifugation and washing. Methods Enzymol. 169, 3-11
19. Root, P., Sliskovic, I. and Mutus, B. (2004) Platelet cell-surface protein disulphide-isomerase mediated S-nitrosoglutathione consumption. Biochem. J. 382,575-580
20. Lahav, J., Jurk, K., Hess, O., Barnes, M. J., Farndale, R. W., Luboshitz, J. and Kehrel, B. E. (2002) Sustained integrin ligation involves extracellular free sulthydryls and enzymatically catalyzed disulphide exchange. Blood 100, 2472-2478
21. Churchich, J. E. (1993) Fluorescence properties of O-aminobenzoyl- labelled proteins. Anal. Biochem. 213, 229-233
22. Holmgren, A. (1979) Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide. J. Biol. Chem. 254, 9627-9632
23. Chen, K., Detwiler, T. C. and Essex, D. W. (1995) Characterization of protein disulphide isomerase released from activated platelets. Br. J. Haematol. 90, 425-431
24. Raturi, A. and Mutus, B. (2004) Use of 2,3-diaminonaphthalene for studying denitrosation activity of protein disulfide isomerase. Anal. Biochem. 326, 281-283
25. Essex, D. W., Chen, K. and Swiatkowska, M. (1995) Localization of protein disulfide isomerase to the external surface of the platelet plasma membrane. Blood 86, 2168-2173
26. Kim, T. W., Yoon, H. Y., Park, J. H., Kwon, O. H., Jang, D. J. and Hong, J. I. (2005) Molecular tripods showing fluorescence enhancement upon binding to streptavidin. Org. Lett. 7, 111-114
27. Lakowicz, J. R., Malicka, J., D'Auria, S. and Gryczynski, I. (2003) Release of the self-quenching of fluorescence near silver metallic surfaces. Anal. Biochem. 320, 13-20
28. Wu, J. H. and Diamond, S. L. (1995) A fluorescence quench and dequench assay of fibrinogen polymerization, fibrinogenolysis, or fibrinolysis. Anal. Biochem. 224, 83-91