Structure and function of lysosomal phospholipase A2: Identification of the catalytic triad and the role of cysteine residues

Journal of Lipid Research

Volumn 46, Issue 11, 2005, Pages 2441-2447

  Hiraoka, Mild ^a,b   Abe, Akira ^a   Shayman, James A ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

Lipase; Macrophage; Phosphatidylcholine; Phosphatidylethanolamine; Phospholipid; Surfactant

Indexed keywords

ACYLTRANSFERASE; AMINO ACID; COMPLEMENTARY DNA; CYSTEINE; LYSOSOME ENZYME; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHOLIPASE A2; PHOSPHOLIPID; SURFACTANT; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CATALYSIS; CELL STRAIN COS7; CONTROLLED STUDY; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE EXPRESSION; IMMUNOBLOTTING; MACROPHAGE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ALANINE; AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; BRAIN; CATALYSIS; CATALYTIC DOMAIN; CATTLE; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY; COS CELLS; CYSTEINE; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTIDINE; HUMANS; IMMUNOBLOTTING; KIDNEY; LYSOSOMES; MACROPHAGES; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OPEN READING FRAMES; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; PHOSPHOLIPASES A; PHOSPHOLIPIDS; PLASMIDS; PULMONARY SURFACTANTS; RATS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; SURFACE-ACTIVE AGENTS; TRANSFECTION;

EID: 27444444715     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: 10.1194/jlr.M500248-JLR200     Document Type: Article

References (22)

1. Abe, A., and J. A. Shayman. 1998. Purification and characterization of 1-O-acylceramide synthase, a novel phospholipase A2 with transacylase activity. J. Biol. Chem. 273: 8467-8474.
2. Hiraoka, M., A. Abe, and J. A. Shayman. 2002. Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase. J. Biol. Chem. 277: 10090-10099.
3. Abe, A., M. Hiraoka, S. Wild, S. E. Wilcoxen, R. Paine 3rd, and J. A. Shayman. 2004. Lysosomal phospholipase A2 is selectively expressed in alveolar macrophages. J. Biol. Chem. 279: 42605-42611.
4. Dranoff, G., A. D. Crawford, M. Sadelain, B. Ream, A. Rashid, R. T. Bronson, G. R. Dickersin, C. J. Bachurski, E. L. Mark, J. A. Whitsett, et al. 1994. Involvement of granulocyte-macrophage colony-stimulating factor in pulmonary homeostasis. Science. 264: 713-716.
5. Abe, A., J. A. Shayman, and N. S. Radin. 1996. A novel enzyme that catalyzes the esterification of N-acetylsphingosine. Metabolism of C2-ceramides. J. Biol. Chem. 271: 14383-14389.
6. Jonas, A. 2000. Lecithin cholesterol acyltransferase. Biochim. Biophys. Acta. 1529: 245-256.
7. Yang, C. Y., D. Manoogian, Q. Pao, F. S. Lee, R. D. Knapp, A. M. Gotto, Jr., and H. J. Pownall. 1987. Lecithin:cholesterol acyltransferase. Functional regions and a structural model of the enzyme. J. Biol. Chem. 262: 3086-3091.
8. Adimoolam, S., and A. Jonas. 1997. Identification of a domain of lecithin-cholesterol acyltransferase that is involved in interfacial recognition. Biochem. Biophys. Res. Commun. 232: 783-787.
9. Jin, L., J. J. Shieh, E. Grabbe, S. Adimoolam, D. Durbin, and A. Jonas. 1999. Surface plasmon resonance biosensor studies of human wild-type and mutant lecithin cholesterol acyltransferase interactions with lipoproteins. Biochemistry. 38: 15659-15665.
10. Warrens, A. N., M. D. Jones, and R. I. Lechler. 1997. Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest. Gene. 186: 29-35.
11. Bensadoun, A., and D. Weinstein. 1976. Assay of proteins in the presence of interfering materials. Anal. Biochem. 70: 241-250.
12. Pollastri, G., and A. McLysaght. 2005. Porter: a new, accurate server for protein secondary structure prediction. Bioinformatics. 21: 1719-1720.
13. Nardini, M., and B. W. Dijkstra. 1999. Alpha/beta hydrolase fold enzymes: the family keeps growing. Curr. Opin. Struct. Biol. 9: 732-737.
14. Peelman, F., N. Vinaimont, A. Verhee, B. Vanloo, J. L. Verscheide, C. Labeur, S. Seguret-Mace, N. Duverger, G. Hutchinson, J. Vandekerckhove, et al. 1998. A proposed architecture for lecithin cholesterol acyl transferase (LCAT): identification of the catalytic triad and molecular modeling. Protein Sci. 7: 587-599.
15. Peelman, F., M. Goethals, B. Vanloo, C. Labeur, R. Brasseur, J. Vandekerckhove, and M. Rosseneu. 1997. Structural and functional properties of the 154-171 wild-type and variant peptides of human lecithin-cholesterol acyltransferase. Eur. J. Biochem. 249: 708-715.
16. Wang, J., A. K. Gebre, R. A. Anderson, and J. S. Parks. 1997. Amino acid residue 149 of lecithin:cholesterol acyltransferase determines phospholipase A2 and transacylase fatty acyl specificity. J. Biol. Chem. 272: 280-286.
17. Peelman, F., B. Vanloo, O. Perez-Mendez, A. Decout, J. L. Verschelde, C. Labeur, N. Vinaimont, A. Verhee, N. Duverger, R. Brasseur, et al. 1999. Characterization of functional residues in the interfacial recognition domain of lecithin cholesterol acyltransferase (LCAT). Protein Eng. 12: 71-78.
18. van Tilbeurgh, H., M. P. Egloff, C. Martinez, N. Rugani, R. Verger, and C. Cambillau. 1993. Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography. Nature. 362: 814-820.
19. Wong, H., and M. C. Schotz. 2002. The lipase gene family. J. Lipid Res. 43: 993-999.
20. Bonelli, F. S., and A. Jonas. 1989. Reaction of lecithin cholesterol acyltransferase with water-soluble substrates. J. Biol. Chem. 264: 14723-14728.
21. Jaeger, K. E., B. W. Dijkstra, and M. T. Reetz. 1999. Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of upases. Annu. Rev. Microbiol. 53: 315-351.
22. 2+-catalyzed oxidation. Biochim. Biophys. Acta. 985: 38-44.