DNA integrity is compromised in protamine-deficient human sperm

Journal of Andrology

Volumn 26, Issue 6, 2005, Pages 741-748

  Aoki, Vincent W ^a   Moskovtsev, Sergey I ^b   Willis, Jennifer ^b   Liu, Lihua ^a   Mullen, J Brendan M ^b   Carrell, Douglas T ^a,c  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b MOUNT SINAI HOSPITAL   (Canada)

^c UNIVERSITY OF UTAH   (United States)

Author keywords

Chromatin; DFI; DNA damage

Indexed keywords

DNA FRAGMENT; NUCLEAR PROTEIN; PROTAMINE;

ADULT; ARTICLE; CHROMATIN STRUCTURE; DNA DAMAGE; DNA INTEGRATION; EMBRYO DEVELOPMENT; HIGH RISK PATIENT; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEFICIENCY; SEMEN ANALYSIS; SPERMATOGENESIS; TRANSCRIPTION REGULATION;

DNA FRAGMENTATION; HUMANS; INFERTILITY, MALE; MALE; PROTAMINES; REPRODUCIBILITY OF RESULTS; SPERMATOZOA;

EID: 26944466235     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: 10.2164/jandrol.05063     Document Type: Article

References (43)

1. Aoki VW, Carrell DT. Human protamines and the developing spermatid: their structure, function, expression and relationship with male infertility. Asian J Androl. 2003;5:315-324.
2. Aoki VW, Liu L, Carrell DT. Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males. Hum Reprod. 2005;20:1298-1306.
3. Balhorn R, Brewer L, Corzett M. DNA condensation by protamine and arginine-rich peptides: analysis of toroid stability using single DNA molecules. Mol Reprod Dev. 2000;56:230-234.
4. Balhorn R, Reed S, Tanphaichitr N. Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males. Experientia. 1988;44:52-55.
5. Belokopytova IA, Kostyleva EI, Tomilin AN, Vorob'ev VI. Human male infertility may be due to a decrease of the protamine p2 content in sperm chromatin. Mol Reprod Dev. 1993;34:53-57.
6. Bench GS, Friz AM, Corzett MH, Morse DH, Balhorn R. DNA and total protamine masses in individual sperm from fertile mammalian subjects. Cytometry. 1996;23:263-271.
7. Bianchi PG, Manicardi GC, Bizzaro D, Bianchi U, Sakkas D. Effect of deoxyribonucleic acid protamination on fluorochrome staining and in situ nick-translation of murine and human mature spermatozoa. Biol Reprod. 1993;49:1083-1088.
8. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod. 2004;19:1401-1408.
9. Carrell DT, Liu L. Altered protamine 2 expression is uncommon in donors of known fertility, but common among men with poor fertilizing capacity, and may reflect other abnormalities of spermiogenesis. J Androl. 2001;22:604-610.
10. Chevaillier P, Mauro N, Feneux D, Jouannet P, David G. Anomalous protein complement of sperm nuclei in some infertile men. Lancet. 1987;2:806-807.
11. Cho C, Jung-Ha H, Willis WD, Goulding EH, Stein P, Xu Z, Schultz RM, Hecht NB, Eddy EM. Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice. Biol Reprod. 2003;69:211-217.
12. Cho C, Willis WD, Goulding EH, Jung-Ha H, Choi YC, Hecht NB, Eddy EM. Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nat Genet. 2001;28:82-86.
13. Corzett M, Mazrimas J, Balhorn R. Protamine 1: protamine 2 stoichiometry in the sperm of eutherian mammals. Mol Reprod Dev. 2002; 61:519-527.
14. Dadoune JP. The nuclear status of human sperm cells. Micron. 1995;26:323-345.
15. de Yebra L, Ballesca JL, Vanrell JA, Bassas L, Oliva R. Complete selective absence of protamine p2 in humans. J Biol Chem. 1993;268:10553-10557.
16. de Yebra L, Ballesca JL, Vanrell JA, Corzett M, Balhorn R, Oliva R. Detection of p2 precursors in the sperm cells of infertile patients who have reduced protamine p2 levels. Fertil Steril. 1998;69:755-759.
17. Evenson D, Jost L. Sperm chromatin structure assay is useful for fertility assessment. Methods Cel Sci. 2000;22:169-189.
18. Evenson D, Jost L, Varner D. Stallion sperm nuclear protamine -sh status and susceptibility to DNA denaturation are not strongly correlated. J Reprod Fert Suppl. 2000;56:401-406.
19. Evenson DP, Jost LK, Baer RK, Turner TW, Schrader SM. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod Toxicol. 1991;5:115-125.
20. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, de Angelis P, Claussen OP. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14:1039-1049.
21. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl. 2002;23:25-43.
22. Fischer MA, Willis J, Zini A. Human sperm DNA integrity: correlation with sperm cytoplasmic droplets. Urology. 2003;61:207-211.
23. Fuentes-Mascorro G, Serrano H, Rosado A. Sperm chromatin. Arch Androl. 2000;45:215-225.
24. Henkel R, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, Gips H, Schill WB, Kruger TF. Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril. 2004;81:965-972.
25. Hud NV, Milanovich FP, Balhorn R. Evidence of novel secondary structure in DNA-bound protamine is revealed by raman spectroscopy. Biochemistry. 1994;33:7528-7535.
26. Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21:33-44.
27. Khara KK, Vlad M, Griffiths M, Kennedy CR. Human protamines and male infertility. J Assist Reprod Genet. 1997;14:282-290.
28. Kosower NS, Katayose H, Yanagimachi R. Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei. J Androl. 1992;13:342-348.
29. Larson KL, DeJonge CJ, Barnes AM, Jost LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod. 2000;15:1717-1722.
30. Love CC, Kenney RM. Scrotal heat stress induces altered sperm chromatin structure associated with a decrease in protamine disulfide bonding in the stallion. Biol Reprod. 1999;60:615-620.
31. Manicardi GC, Bianchi PG, Pantano S, Azzoni P, Bizzaro D, Bianchi U, Sakkas D. Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin a3 accessibility. Biol Reprod. 1995;52:864-867.
32. Mengual L, Ballesca JL, Ascaso C, Oliva R. Marked differences in protamine content and p1/p2 ratios in sperm cells from Percoll fractions between patients and controls. J Androl. 2003;24:438-447.
33. Muratori M, Piomboni P, Baldi E, Filimberti E, Pecchioli P, Moretti E, Gambera L, Baccetti B, Biagiotti R, Forti G, Maggi M. Functional and ultrastructural features of DNA-fragmented human sperm. J Androl. 2000;21:903-912.
34. Nasr-Esfahani MH, Razavi S, Mozdarani H, Mardani M, Azvagi H. Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI. Andrologia. 2004;36:95-100.
35. Oliva R, Dixon GH. Vertebrate protamine genes and the histone-to-protamine replacement reaction. Prog Nucleic Acid Res Mol Biol. 1991; 40:25-94.
36. Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril. 2004;82:378-383.
37. Sharma RK, Said T, Agarwal A. Sperm DNA damage and its clinical relevance in assessing reproductive outcome. Asian J Androl. 2004; 6:139-148.
38. Sun JG, Jurisicova A, Casper RF Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997;56:602-607.
39. Tomlinson MJ, Moffatt O, Manicardi GC, Bizzaro D, Afnan M, Sakkas D. Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception. Hum Reprod. 2001;16:2160-2165.
40. Tomsu M, Sharma V, Miller D. Embryo quality and IVF treatment outcomes may correlate with different sperm comet assay parameters. Hum Reprod. 2002;17:1856-1862.
41. Virant-Klun I, Tomazevic T, Meden-Vrtovec H. Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J Assist Reprod Genet. 2002;19:319-328.
42. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. 2004;81:1289-1295.
43. Zini A, Kamal K, Phang D, Willis J, Jarvi K. Biological variability of sperm DNA denaturation in infertile men. Urology. 2001;58:258-261.