Synthesis and biological evaluation of novel bisheterocycle-containing compounds as potential anti-influenza virus agents

Bioorganic and Medicinal Chemistry Letters

Volumn 15, Issue 23, 2005, Pages 5284-5287

  Wang, Wen Long ^a   Yao, De Yong ^b   Gu, Min ^a   Fan, Min Zhi ^a   Li, Jing Ya ^a   Xing, Ya Cheng ^b   Nan, Fa Jun ^a  

^a SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

^b QINGDAO UNIVERSITY   (China)

Author keywords

Anti influenza virus; Bisheterocycle containing compounds; Synthesis

Indexed keywords

4,2 BISHETEROCYLE DERIVATIVE; HETEROCYCLIC COMPOUND; INFLUENZA VACCINE; OXAZOLE DERIVATIVE; THIAZOLE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; DRUG SCREENING; DRUG SYNTHESIS; INFLUENZA VIRUS A; INHIBITION KINETICS; NONHUMAN;

ANTIVIRAL AGENTS; HUMANS; INFLUENZAVIRUS A; OXAZOLES; THIAZOLES;

INFLUENZA A VIRUS; INFLUENZA VIRUS;

EID: 26844541494     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.08.046     Document Type: Article

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15. 2O (20 mL). The aqueous phase was extracted with EtOAc (4×20 mL). The combined organic phases were then processed in the usual way and chromatographed to yield 2f (29 mg, 86%).
16. MDCK cells were grown as specified in Eagle's minimum essential medium with 10% heat-inactivated fetal bovine serum (FBS) plus antibiotics (penicillin, 100 U/mL; streptomycin, 100 U/mL). Influenza A H3N2 viruses (A3 China/15/90) were propagated in the allantoic cavities of 10-day-old embryonated eggs. Virus titers were determined by hemagglutinin titration, according to standard procedures. Confluent MDCK monolayers were infected with Influenza A viruses for 2 h at 37°C, after which the viral inoculum was removed and cells were treated with different concentrations of compound. When CPE result of the viral control group reached 4+, the result of compound treated group was observed. The dilution that gives 50% cytopathic effect was determined by the interpolating procedure of Reed and Muench.