Cytochrome P450BM-3 mutants with improved catalytic properties of hydroxylating indole to indigo by error-prone PCR

Progress in Biochemistry and Biophysics

Volumn 32, Issue 7, 2005, Pages 630-635

  Li, Hong Mei ^a   Mei, Le He ^a   Urlacher, Vlada ^b   Schmid, Rolf D ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Catalytic activity; Cytochrome P450BM 3; Directed evolution; Error prone PCR; Indigo

Indexed keywords

BACILLUS MEGATERIUM;

EID: 26844540485     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Elizabeth M, Gillam J, Lisa M N, et al. Oxidation of indole by cytochrome P450 enzymes. Biochemistry. 2000, 39(45): 13817-13824
2. Meyer A, Wursten M, Schmid A, et al. Hydoxylation of indole by laboratory-evolved 2- hydroxybiphenyl 3-monooxygenase. J Biol Chem, 2002, 277 (37): 34161-34167
3. Li Q S, Schwaneberg U, Schmid R D, et al. Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. Chem Eur J, 2000, 6 (9): 1531-1536
4. Budde M, Maurer S C, Schmid R D, et al. Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtility. Applied Microbiology Biotechnology, 2004, 66 (2): 180-186
5. Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomers. J Biol Chem, 1964, 239 (7): 2370-2378
6. Schwaneberg U, Schmidt D C, Schmid R D, et al. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. Analytical Biochemistry, 1999, 4047 (269): 359-366
7. Xu H F, Zhang X E, Zhang Z P, et al. Prog Biochem Biophys, 2003, 30 (1): 89-94