Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography-digestion-liquid chromatography-electrospray mass spectrometry

Analytical Biochemistry

Volumn 346, Issue 1, 2005, Pages 150-157

  Carol, Jeroen ^a,b   Gorseling, Maarten C J K ^a   De Jong, Camiel F ^a   Lingeman, Henk ^a   Kientz, Charles E ^b   Van Baar, Ben L M ^b   Irth, Hubertus ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b TNO   (Netherlands)

Author keywords

Biological toxins; Liquid chromatography; Mass spectrometry; On line digestion; Size exclusion chromatography

Indexed keywords

AUTOMATION; DESALINATION; DISEASES; DRUG PRODUCTS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; LIQUIDS; MAMMALS; MASS SPECTROMETRY; PROTEINS;

BIOLOGICAL TOXINS; BOVINE SERUM ALBUMINS; IDENTIFICATION OF PROTEINS; INFECTIOUS DISEASE; LIQUID CHROMATOGRAPHY ELECTROSPRAY; ON-LINE DIGESTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE COVERAGES;

SIZE EXCLUSION CHROMATOGRAPHY;

BOVINE SERUM ALBUMIN; CHOLERA TOXIN; MYOGLOBIN; STAPHYLOCOCCUS ENTEROTOXIN;

ARTICLE; DESALINATION; DIGESTION; ELECTROSPRAY MASS SPECTROMETRY; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

BOVINAE;

EID: 26844528419     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.08.023     Document Type: Article

References (21)

1. D.R. Franz, P.B. Jahrling, A.M. Friedlander, D.J. McClain, D.L. Hoover, W.R. Bryne, J.A. Pavlin, G.W. Christopher, and E.M. Eitzen Clinical recognition and management of patients exposed to biological warfare agents J. Am. Med. Assoc. 278 1997 399 411
2. K.J. Towner A. Cockayne Molecular Methods for Microbial Identification and Typing 1993 Chapman and Hall London
3. R. Kittelberger, P.G. Bundesen, A. Cloeckaert, I. Greiser-Wilke, and J.J. Letesson Serological cross-reactivity between Brucella abortus and Yersinia enterocolitica 0:9: IV. Evaluation of the M- and C-epitope antibody response for the specific detection of B. abortus infections Vet. Microbiol. 60 1998 45 57
4. F.R. Cockerill 3rd Application of rapid-cycle real-time polymerase chain reaction for diagnostic testing in the clinical microbiology laboratory Arch. Pathol. Lab. Med. 127 2003 1112 1120
5. S.J. Cordwell, A.S. Nouwens, and B.J. Walsh Comparative proteomics of bacterial pathogens Proteomics 1 2001 461 472
6. W.H. McDonald, and J.R. Yates 3rd Shotgun proteomics: integrating technologies to answer biological questions Curr. Opin. Mol. Ther. 5 2003 302 309
7. J.F. Nemeth-Cawley, B.S. Tangarone, and B.S. Rouse "Top down" characterization is a complementary technique to peptide sequencing for identifying protein species in complex mixtures J. Prot. Res. 2 2003 495 505
8. D.A. Wolters, M.P. Washburn, and J.R. Yates 3rd An automated multidimensional protein identification technology for shotgun proteomics Anal. Chem. 73 2001 5683 5690
9. M.P. Molley, E.E. Brzezinski, J. Hang, M.T. McDowell, and R.A. Van Bogelen Overcoming technical variation and biological variation in quantitative proteomics Proteomics 3 2003 1912 1919
10. M. Ünlü, M.E. Morgan, and J.S. Minden Difference gel electrophoresis: a single gel method for detecting changes in protein extracts Electrophoresis 18 1997 2071 2077
11. E.P. Romijn, J. Krijgsveld, and A.J.R. Heck Recent liquid chromatographic-(tandem) mass spectrometric applications in proteomics J. Chromatogr. A 1000 2003 589 608
12. Y. Shi, R. Xiang, C. Horváth, and J.A. Wilkins The role of liquid chromatography in proteomics J. Chromatogr. A 1053 2004 27 36
13. Y. Ishihama Proteomics LC-MS systems using nanoscale liquid chromatography with tandem mass spectrometry J. Chromatogr. A 1067 2005 73 83
14. B.L.M. van Baar, A.G. Hulst, B. Roberts, and E.R.J. Wils Characterization of tetanus toxin, neat and in culture supernatant, by electrospray mass spectrometry Anal. Biochem. 301 2002 278 289
15. S. Beranova-Giorgianni Proteome analysis by two dimensional gel electrophoresis and mass spectrometry: strengths and limitations Trends Anal. Chem. 22 2003 273 281
16. N.F.C. Visser, H. Lingeman, K.W. Li, and H. Irth Quantitative transfer of in-gel digest products to liquid chromatography-electrospray mass spectrometry using on-line coupled extraction Chromatographia 61 2005 433 442
17. P. Lecchi, A.R. Gupte, R.E. Perez, L.V. Stockert, and F.P. Abramson Size-exclusion chromatography in multidimensional separation schemes for proteome analysis J. Biochem. Biophys. Methods 56 2003 141 152
18. D.N. Perkins, D.J. Pappin, D.M. Creasy, and J.S. Cottrell Probability-based protein identification by searching sequence databases using mass spectrometry data Electrophoresis 20 1999 3551 3567
19. C.E. Kientz, A.G. Hulst, and E.R.J. Wils Determination of Staphylococcal Enterotoxin B by on-line (micro) liquid chromatography-electrospray mass spectrometry J. Chromatogr. A 757 1997 51 64
20. B.L.M. van Baar, A.G. Hulst, and E.R.J. Wils Characterisation of cholera toxin by liquid chromatography-electrospray mass spectrometry Toxicon 37 1999 85 108
21. G.J. Opiteck, J.W. Jorgenson, and R.J. Anderegg Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides Anal. Chem. 69 1997 2283 2291