Terminal phosphate labeled nucleotides: Synthesis, applications, and linker effect on incorporation by DNA polymerases

Nucleosides, Nucleotides and Nucleic Acids

Volumn 24, Issue 5-7, 2005, Pages 401-408

  Kumar, Shiv ^a   Sood, Anup ^a   Wegener, Jeffery ^a   Finn, Patrick J ^a   Nampalli, Satyam ^a   Nelson, John R ^a   Sekher, Anuradha ^a   Mitsis, Paul ^a   Macklin, John ^a   Fuller, Carl W ^a  

^a GE HEALTHCARE   (United States)

Author keywords

DNA Polymerase Extension; Dyes; Homogenous Assays; Linkers; Terminal Phosphate Labeled Nucleotides

Indexed keywords

DNA POLYMERASE; DYE; NUCLEOTIDE DERIVATIVE; RNA POLYMERASE; TERMINAL PHOSPHATE LABELED NUCLEOTIDE DERIVATIVE; UNCLASSIFIED DRUG;

BIOCHEMISTRY; CHEMICAL LABELING; CONFERENCE PAPER; DIAGNOSTIC VALUE; DNA SEQUENCE; DNA STRUCTURE; DRUG SYNTHESIS; ENZYME SUBSTRATE; ENZYME SYNTHESIS; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM;

COLORING AGENTS; DNA; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; GENETIC TECHNIQUES; MODELS, CHEMICAL; NUCLEOTIDES; PHOSPHATES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; TIME FACTORS;

EID: 26644436048     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1081/NCN-200059823     Document Type: Conference Paper

References (12)

1. Nampalli, S.; Khot, M.; Kumar, S. Fluorescence energy transfer terminators for DNA sequencing. Tetrahedron. Lett. 2000, 41, 8867-8871.
2. Rosenblum, B.B.; Lee, L.G.; Spurgeon, S.L.; Khan, S.H.; Menchen, S.M.; Heiner, C.R.; Chen, S.M. New dye-labeled terminators for improved DNA sequencing patterns. Nucleic Acids Res. 1997, 25, 4500-4504.
3. Zhao, X.; Nampalli, S.; Serino, A.; Kumar, S. Immobilization of oligonucleotides with multiple anchors to microchips. Nucleic Acids Res. 2001, 29, 955-959.
4. Kumar, S. Fluorescent dye nucleotide conjugates for DNA sequencing. In Modified Nucleotides, Synthesis and Applications. Loakes, D., Ed.; Research Signpost: Trivandrum, India, 2002; 87-100.
5. Arzumanov, A.A.; Semizarov, D.G.; Victorova, L.S.; Dyatkina, N.B.; Krayevsky, A.A. γ-Phosphate-substituted 2′-deoxynucleotide 5′-triphosphates as substrates for DNA polymerases. J. Biol. Chem. 1996, 271(40), 24389-24394.
6. Knorre, D.G.; Kurbatov, V.A.; Samukov, V.V. FEBS Lett. 1976, 70(1), 105-108.
7. Finn, P.; Bull, M.; Xiao, H.; Nelson J.R.; Mamone, A.; Grossmann, G.; Nampalli, S.; Flick, P.; Fuller, C.W.; Kumar, S. Efficient incorporation of positively charged 2′,3′-dideoxynucleoside-5′-triphosphates by DNA polymerases and their application to "direct-load" DNA sequencing. Nucleic Acids Res. 2003, 31, 4769-4778.
8. Finn, P.; Sun, L.; Nampalli, S.; Xiao, H.; Nelson J.R.; Mamone, A.; Grossmann, G.; Flick, P.; Fuller, C.W.; Kumar, S. Synthesis and application of charge-modified dye labeled dideoxynucleoside-5′-triphosphates to "direct-load" DNA sequencing. Nucleic Acids Res. 2002, 30, 2877-2885.
9. Sood, A.; Kumar, S.; Nelson J.R.; Fuller, C.W. Solid phase sequencing. 2003.
10. Sood, A.; Kumar, S.; Nelson J.R.; Fuller, C.W. Allele specific primer extension. 2004.
11. Sood, A.; Kumar, S.; Nelson J.R.; Fuller, C.W. Analyte detection. 2004.
12. Sood, A.; Kumar, S.; Nelson J.R.; Fuller, C.W.; Sekher, A. Nucleic acid amplification. 2004.