Efficient incorporation of positively charged 2′, 3′-dideoxynucleoside-5′-triphosphates by DNA polymerases and their application in 'direct-load' DNA sequencing

Nucleic Acids Research

Volumn 31, Issue 16, 2003, Pages 4769-4778

  Finn, Patrick J ^a   Bull, Matthew G ^a   Xiao, Haiguang ^a   Phillips, Paula D ^a   Nelson, John R ^a   Grossman, Greg ^a   Nampalli, Satyam ^a   McArdle, Bernard F ^a   Mamone, J Anthony ^a   Flick, Parke K ^a   Fuller, Carl W ^a   Kumar, Shiv ^a  

^a Amersham Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; DNA POLYMERASE; DYE; NUCLEOTIDE; REAGENT;

ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; CULTURE MEDIUM; DNA MODIFICATION; DNA PURIFICATION; DNA SEQUENCE; ELECTROPHORESIS; GEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; SEPARATION TECHNIQUE; SYNTHESIS; TRANSPORT KINETICS;

BASE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORING AGENTS; DIDEOXYNUCLEOSIDES; DNA; DNA-DIRECTED DNA POLYMERASE; LYSINE; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; NUCLEOTIDES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 10744228957     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg669     Document Type: Article

References (16)

1. International Human Genome Mapping Consortium (2001) A physical map of the human genome. Nature, 409, 934-941.
2. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
3. Smith, L.M., Fung, S., Hunkapiller, M.W., Hunkapiller, T.J. and Hood, L.E. (1985) The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Res., 13, 2399-2412.
4. Voss, H., Schwager, C., Wirkner, U., Sproat, B., Zimmermann, J., Rosenthal, A., Erfele, H., Stegemann, J. and Ansorge, W. (1989) Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA. Nucleic Acids Res., 17, 2517-2527.
5. Bergot, B.J., Chakerian, V., Connell, C.R., Eadie, J.S., Fung, S., Hershey, N.D., Lee, L.G., Menchen, S.M. and Woo, S.L., (1994) US Patent No. 5 366 860.
6. Reeve, M.A. and Fuller, C.W. (1995) A novel thermostable polymerase for DNA sequencing. Nature, 376, 796-797.
7. Tabor, S. and Richardson, C.C. (1990) Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis. J. Biol. Chem., 264, 5447-6458.
8. Tabor, S. and Richardson, C.C. (1990), DNA sequence analysis with a modified bacteriophage T7 DNA polymerase - effect of pyrophosphorolysis and metal ions. J. Biol. Chem., 265, 8322-8328.
9. Hobbs, F.W., Jr and Cocuzza, A.J. (1991) US Patent No. 5 047 519.
10. Smith, L.M., Sanders, J.Z., Kaiser, R.J., Hughes, P., Doss, C., Conell, C.R., Heiner, C., Kents, S.B.H. and Hood, L. (1986), Fluorescence detection in automated DNA sequencing. Nature, 321, 674-678.
11. Prober, G.M., Trainor, G.L., Dam, R.J., Hobbs, F.W., Robertson, C.W., Zagursky, R.J., Cocuzza, A.J., Jensen, M.A. and Baumeister, K. (1987) A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science, 238, 336-341.
12. Swedlow, H., Zhang, J.Z., Chen, D.Y., Harke, H.R., Grey, R., Wu, S. and Dovichi, N., (1991) Three DNA sequencing methods based on capillary gel electrophoresis and laser-induced fluorescence. Anal. Chem., 63, 2835-2841.
13. Brazill, S.A., Kim, P.H. and Kuhr, W.G. (2001) Capillary gel electrophoresis with sinusoidal voltammetric detection: a strategy to allow four-'color' DNA sequencing. Anal. Chem., 73, 4882-4890.
14. Finn, P.J., Sun, L., Nampalli, S., Xiao, H., Nelson, J.R., Mamone, J.A., Grossmann, G., Flick, P.K., Fuller, C.W. and Kumar, S. (2002) Synthesis and application of charge modified dye-labeled dideoxynucleoside-5′-triphosphates to 'direct-load' DNA sequencing. Nucleic Acids Res., 30, 2877-2885.
15. Lee, L.G., Connell, C.R., Woo, S.L., Cheng, R.D., McArdle, B.F., Fuller, C.W., Halloran, N.D. and Wilson, R.K. (1992) DNA sequencing with dye-labeled terminators and T7 polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments. Nucleic Acids Res., 20, 2471-2483.
16. Nampalli, S., Khot, M. and Kumar, S. (2000) Fluorescence energy transfer terminators for DNA sequencing. Tetrahedron Lett., 41, 8867-8871.