The effect of different thawing methods, growth factor combinations and media on the ex vivo expansion of umbilical cord blood primitive and committed progenitors

Bone Marrow Transplantation

Volumn 21, Issue 3, 1998, Pages 233-241

  Kogler G ^a   Callejas, J ^a   Sorg, R V ^a   Fischer, J ^a   Migliaccio, A R ^b   Wernet, P ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Cord blood; Ex vivo expansion; Flt3 ligand

Indexed keywords

CD34 ANTIGEN; ERYTHROPOIETIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 3; STEM CELL FACTOR;

ARTICLE; BURST FORMING UNIT E; COLONY FORMING CELL; COLONY FORMING UNIT E; COLONY FORMING UNIT GEMM; COLONY FORMING UNIT GM; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; THAWING; UMBILICAL CORD BLOOD;

EID: 2642714929     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bmt.1701088     Document Type: Article

References (35)

1. Rubinstein P, Carrier C, Adamson J et al. New York Blood Center's program for unrelated placental/umbilical cord blood transplantation: 243 transplants in the first three years. Blood 1996; 88: 142a (Abstr).
2. Kurtzberg J, Laughlin M, Graham ML et al. Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. New Engl J Med 1996; 335: 157-166.
3. Gluckman E, Broxmeyer HE, Auerbach AD et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of an UCB from an HLA-identical sibling. New Engl J Med 1989; 321: 1174-1178.
4. Wagner JE, Broxmeyer HE, Byrd RL et al. Transplantation of umbilical cord blood after myeloablative therapy: analysis of engraftment. Blood 1992; 79: 1874-1881.
5. Migliaccio AR, Adamson JW, Rubinstein P et al. Correlation between progenitor cell dose, likelihood to engraft and time to myeloid engraftment in 79 unrelated placental/cord blood transplants. Acta Haematol 1997; 98 (Suppl. 1): 31 (Abstr.).
6. Gluckman E, Thierry D, Traineau R. Blood banking for hematopoietic stem cell transplantation. J Hematother 1993; 2: 269-270.
7. Bertolini F, Lazzari L, Lauri E et al. Comparative study of different procedures for the collection and banking of umbilical cord blood. J Hematother 1995; 4: 29-36.
8. Rubinstein P, Taylor PE, Scaradavou A et al. Unrelated placental blood for bone marrow reconstitution: organization of the placental blood program. Blood Cells 1994; 20: 587-596.
9. Kögler G, Callejas J, Hakenberg P et al. Hematopoietic transplant potential of unrelated cord blood: critical issues. J Hematother 1996; 5: 105-116.
10. Moore MAS, Hoskins I. Ex vivo expansion of cord blood-derived stem cells and progenitors. Blood Cells 1994; 20: 468-479.
11. Broxmeyer HE, Hangoc G, Cooper S et al. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc Natl Acad Sci USA 1992; 89: 4109-4113.
12. + cord blood cells in the presence of recombinant human stem cell factor. Blood 1992; 79: 2620-2627.
13. + cord blood cells in serum-free long-term cultures supplemented with hematopoietic cytokines. Blood 1993; 82: 2664.
14. Williams DA. Ex vivo expansion of hematopoietic stem and progenitor cells: robbing Peter to pay Paul. Blood 1993; 81 3169-3172.
15. Lansdorp PM, Dragowska W. Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors. Exp Hematol 1993; 21: 1321-1327.
16. + cord blood cells. Blood Cells 1994; 20: 129-138.
17. Broxmeyer HE, Lu L, Cooper S et al. Flt3 ligand stimulates/costimulates the growth of myeloid stem/progenitor cells. Exp Hematol 1995; 23: 1121-1129.
18. Yurasov SV, Flasshove M, Rafii SM, Moore MAS. Density enrichment and characterization of hematopoietic progenitors and stem cells from umbilical cord blood. Bone Marrow Transplant 1996; 17: 517-525.
19. Piacibello W, Sanavio F, Garetto L et al. Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood. Blood 1997; 89: 2644-2653.
20. Bhatia M, Bonnet D, Kapp U et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture. J Exp Med 1997; 186: 619-624.
21. - cord blood cells. J Hematother 1997; 6: 93-102.
22. + cells is essential for optimal ex vivo expansion of umbilical cord blood cells. J Hematother 1997; 6: 145-150.
23. Vaziri H, Dragowska W, Allsopp RC et al. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA 1994; 91: 9857-9860.
24. Rubinstein P, Dobrila L, Rosenfield RE et al. Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. Proc Natl Acad Sci USA 1995; 92: 10119-10122.
25. Berardi AC, Wang A, Levine JD et al. Functional isolation and characterization of human hematopoietic stem cells. Science 1995; 267: 104-108.
26. + cells in peripheral and cord blood by flow cytometry. Exp Hematol 1994; 22: 1003-1010.
27. Sutherland HJ, Lansdorp PM, Henkelman DH et al. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive stromal layers. Proc Natl Acad Sci USA 1990; 87: 3584-3588.
28. Hogge DE, Lansdorp PM, Reid D et al. Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor. Blood 1996; 88: 3765-3773.
29. Lyman SD, James L, Van den Bos T et al. Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell 1993; 75: 1157-1167.
30. + hematopoietic progenitor cells. Blood 1995; 86: 3413-3420.
31. Koller MR, Emerson SG, Palsson BO. Large scale expansion of human stem and progenitor cells from bone marrow mononuclear cells in continuous perfusion cultures. Blood 1993; 82: 378-384.
32. Zandstra PW, Eaves CJ, Piret JM. Expansion of hematopoietic progenitor cell population in stirred suspension bioreactors of normal human marrow cells. Biotechnology 1994; 12: 909-914.
33. Ponchio L, Conneally E, Eaves C. Quantitation of quiescent fraction of long-term culture-initiating cells in normal human blood and marrow and the kinetics of their growth factorstimulated entry into S-phase in vitro. Blood 1995; 86: 3314-3321.
34. Sandstrom CE, Collins PC, McAdams TA et al. Comparison of whole serum-deprived media for ex vivo expansion of hematopoietic progenitor cells from cord blood and mobilized peripheral blood mononuclear cells. J Hematother 1996; 5: 461-473.
35. Iscove NN, Guilbert LJ, Weyman C. Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol. Exp Cell Res 1980; 126: 121-126.