Alteration of substrate affinities and specificities of the Chlorella hexose/H+ symporters by mutations and construction of chimeras

Journal of Biological Chemistry

Volumn 273, Issue 19, 1998, Pages 11456-11462

  Will, Andreas ^a   Graßl, Renate ^a   Erdmenger, Jörg ^a   Casparit, Thomas ^a,b   Tanner, Widmar ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF SUSSEX   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GALACTOSE; GLUCOSE; HEXOSE UPTAKE PROTEIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; CHIMERA; CHLORELLA; DNA SEQUENCE; GEL ELECTROPHORESIS; HEXOSE TRANSPORT; IMMUNOBLOTTING; MUTAGENESIS; MUTATION; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; CARRIER PROTEINS; CHIMERA; CHLORELLA; DNA MUTATIONAL ANALYSIS; HEXOSES; HYDROGEN-ION CONCENTRATION; KINETICS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; SYMPORTERS;

BACTERIA (MICROORGANISMS); PARACHLORELLA KESSLERI; SCHIZOSACCHAROMYCES POMBE;

EID: 2642704198     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.19.11456     Document Type: Article

References (38)

1. Tanner, W. (1969) Biochem. Biophys. Res. Commun. 36, 278-283
2. Komor, E. (1973) FEBS Lett. 38, 16-18
3. Komor, E., and Tanner, W. (1974) J. Gen. Physiol. 64, 568-581
4. Komor, E., and Tanner, W. (1976) Eur. J. Biochem. 70, 197-204
5. Sauer, N., and Tanner, W. (1989) FEBS Lett. 259, 43-46
6. Stadler, R., Wolf, K., Hilgarth, C., Tanner, W., and Sauer, N. (1995) Plant Physiol. 107, 33-41
7. Sauer, N., Caspari, T., Klebl, F., and Tanner, W. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 7949-7952
8. Opekarová, M., Caspari, T., and Tanner, W. (1994) Biochim. Biophys. Acta 1194, 149-154
9. Caspari, T., Robl, I., Stolz, J., and Tanner, W. (1996) Plant J. 10, 1045-1053
10. Marger, M. D., and Saier, M. H., Jr. (1993) Trends Biochem. Sci. 18, 13-20
11. Calamia, J., and Manoil, C. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 4937-4941
12. Hresko, R. C., Kruse, M., Strobe, M., and Mueckler, M. (1994) J. Biol. Chem. 269, 20482-20488
13. Caspari, T., Stadler, R., Sauer, N., and Tanner, W. (1994) J. Biol. Chem. 269, 3498-3502
14. Will, A., Caspari, T., and Tanner, W. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 10163-10167
15. Milbradt, B., and Höfer, M. (1994) Microbiol. 140, 2617-2623
16. Quiocho, F. A., and Vyas, N. K. (1984) Nature 310, 381-386
17. Vyas, N. K., Vyas, M. N., and Quiocho, F. A. (1988) Science 242, 1290-1295
18. Will, A., and Tanner, W. (1996) FEBS Lett. 381, 127-130
19. Russel, P., and Nurse, P. (1986) Cell 45, 145-153
20. McLeod, M., Stein, M., and Beach, D. (1987) EMBO J. 6, 729-736
21. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds) (1992) in Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 2nd Ed., pp. 13-43-13-44, John Wiley & Sons, New York
22. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
23. Laemmli, U. K. (1970) Nature 227, 680-685
24. Sahin-Tóth, M., Dunten, R., Gonzalez, A., and Kaback, H. R. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 10547-10551
25. Mueckler, M., Weng, W., and Kruse, M. (1994) J. Biol. Chem. 269, 20533-20538
26. Hashiramoto, M., Kadowaki, T., Clark, A. E., Muraoka, A., Momomura, K., Sakura, H., Tobe, K., Akanuma, Y., Yazaki, Y., Holman, G. D., and Kasuga, M. (1992) J. Biol. Chem. 267, 17502-17507
27. Garcia, J. C., Strube, M., Leingang, K., Keller, K., and Mueckler, M. M. (1992) J. Biol. Chem. 267, 7770-7776
28. Maiden, M. C. J., Davis, E. O., Baldwin, S. A., Moore, D. C. M., and Henderson, P. J. F. (1987) Nature 325, 641-643
29. Tanner, W., and Caspari, T. (1996) Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 595-626
30. Varela, M. F., Brooker, R. J., and Wilson, T. H. (1997) J. Bacteriol. 179, 5570-5573
31. Kaback, H. R. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 5539-5543
32. Sauer, N., Friedländer, K., and Gräml-Wicke, U. (1990) EMBO J. 9, 3045-3050
33. Sauer, N., and Stadler, R. (1993) Plant J. 4, 601-610
34. Weig, A., Franz, J., Sauer, N., and Komor, E. (1994) J. Plant Physiol. 143, 178-183
35. Harrison, M. J. (1996) Plant J. 9, 491-503
36. Nishizawa, K., Shimoda, E., and Kasahara, M. (1995) J. Biol. Chem. 270, 2423-2426
37. Kasahara, M., Shimoda, E., and Maeda, M. (1996) FEBS Lett. 389, 174-178
38. Metzger, T. G., and Ferguson, D. M. (1995) FEBS Lett. 375, 1-4