Purification of the chlorella HUP1 hexose-proton symporter to homogeneity and its reconstitution in vitro

Plant Journal

Volumn 10, Issue 6, 1996, Pages 1045-1053

  Caspari, Thomas ^a   Robl, Ingrid ^a   Stolz, Jürgen ^a,b   Tanner, Widmar ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORELLA; ESCHERICHIA COLI; PROKARYOTA; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0030482429     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.1996.10061045.x     Document Type: Article

References (46)

1. Azzone, G.F., Colonna, R. and Ziche, B. (1979) Preparation of bovine heart mitochondria on high yield. Methods Enzymol. 55, 46-50.
2. Baldwin, J.M., Lienhard, G.E. and Baldwin, S.A. (1980) The monosaccharide transport system of the human erythrocyte. Orientation and reconstitution. Biochim. Biophys. Acta, 599, 699-714.
3. Berhe, A., Fristedt, U. and Perrson, B.L. (1995) Expression and purification of the high-affinity phosphate transporter of Saccharomyces cerevisiae. Eur. J. Biochem. 227, 566-572
4. + channel from Arabidopsis thaliana, and comparison with endogeneous yeast channels and carriers. Proc. Natl Acad. Sci. USA, 92, 2701-2705.
5. Bogdanov, M. and Dowhan, W. (1995) Phosphatidylethanolamine is required for in vivo function of the membrane-associated lactose permease of Escherichia coli. J. Biol. Chem. 270, 732-739.
6. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
7. + symporter. J. Biol. Chem. 269, 3498-3502.
8. + symporters in lower and higher plants. J. Exp. Biol. 196, 483-491.
9. Chapman-Smith, A., Turner, D.L., Cronan, Jr, J.E., Morris, T.W. and Wallace, J.C. (1994) Expression, biotinylation and purification of a biotin-domain peptide from the biotin carrier protein of Escherichia coli acetyl-CoA carboxylase. Biochem. J. 302, 881-887.
10. Chen, C.-C. and Wilson, T.H. (1984) The phospholipid requirement for activity of the lactose carrier of Escherichia coli. J. Biol. Chem. 259, 10 150-10 158.
11. Consler, T.G., Persson, B.L., Jung, H., Zen, K.H., Jung, K., Privé, G.G., Verner, G.E. and Kaback, H.R. (1993) Properties and purification of an active biotinylated lactose permease from Escherichia coli. Proc. Natl Acad. Sci. USA, 90, 6934-6938.
12. Cronan, Jr, J.E. (1990) Biotination of proteins in vivo. A posttranslational modification to lable, purify and study proteins. J. Biol. Chem. 265, 10 327-10 333.
13. Driessen, A.J.M. and Konings, W.N. (1993) Insertion of lipids and proteins into bacterial membranes by fusion with liposomes. Methods Enzymol. 221, 394-408.
14. Dufour, J.-P., Amory, A. and Goffeau, A. (1988) Plasma membrane ATPase from the yeast Schizosaccharomyces pombe. Methods Enzymol. 157, 513-528.
15. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
16. Hyo, I.C. and Cohen, N.D. (1983) Regulation and intracellular localization of the biotin holocarboxylase synthetase of 3T3-L1 cells. Arch. Biochem. Biophys. 225, 237-247.
17. Komor, E. (1973) Proton-coupled hexose transport in Chlorella vulgaris. FEBS Lett. 38, 16-18.
18. Komor, E. and Tanner, W. (1974) The hexose-proton symport system of Chlorella vulgaris. Specificity, stoichometry and energetics of sugar-induced proton uptake. Eur. J. Biochem. 44, 219-233.
19. Komor, E., Haass, D., Komor, B. and Tanner, W. (1973) The active hexose-uptake system of Chlorella vulgaris: Km-values for 6-deoxyglucose influx and efflux and their contribution to sugar accumulation. Eur. J. Biochem. 39, 193-200.
20. Komor, E., Schobert, C. and Cho, B.H. (1983) The hexose uptake system of Chlorella: Is it a proton symport or a hydroxyl ion antiport system? FEBS Lett. 156, 6-10.
21. Kuboyama, W., Yong, F.C. and King, T.E. (1972) Studies on cytochrome oxidase. VIII. Preparation and some properties of cardiac cytochrome oxidase. J. Biol. Chem. 247, 6375-6383.
22. Leupold, U. (1950) Die Vererbung von Homothallie und Heterothallie bei Schizosaccharomyces pombe. C. R. Trav. Lab. Carlsberg. Ser. Physiol. 24, 381-480.
23. Lim, F., Rode, M., Morris, P.C. and Wallace, J.C. (1987) Pyruvate carboxylase in the yeast pyc mutant. Arch. Biochem. Biophys. 258, 259-264.
24. Loddenkötter, B., Kammerer, B., Fischer, K. and Flügge, U.-I. (1993) Expression of the functional mature triose phosphate translocator in yeast internal membranes and purification of the histidine-tagged protein by a single metal-affinity chromatography step. Proc. Natl Acad. Sci. USA, 90, 2155-2159.
25. Marger, M.D. and Saier, M.H. (1993) A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biol. Sci. 18, 13-20.
26. Milbradt, B. and Höfer, M. (1994) Glucose-transport-deficient mutants of Schizosaccharomyces pombe: phenotype, genetics and use for genetic complementation. Microbiology, 140, 2617-2623.
27. +/glucose symport studied in vitro. Biochim. Biophys. Acta, 1194, 149-154.
28. Peerce, B.E. and Wright, E.M. (1984) Sodium-induced conformational changes in the glucose transporter of intestinal brush borders. J. Biol. Chem. 259, 14 105-14 112.
29. + sugar symport is catalyzed by a single polypeptide. Biochemistry, 34, 4412-4420.
30. Racker, E., Violand, B., O'Neil, S., Alfonzo, M. and Telford, J. (1979) Reconstitution, a way of biochemical research; some new approaches to membrane-bound enzymes. Arch. Biochem. Biophys. 198, 470-477.
31. Riesmeier, J.W., Willmitzer, L. and Frommer, W.B. (1992) Isolation and characterisation of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO J. 11, 4705-4713.
32. Robertson, D.E., Kaczorowski, G.J., Garcia, M.-L. and Kaback, H.R. (1980) Active transport in membrane vesicles from Escherichia coli. The electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states. Biochemistry, 19, 5692-5702.
33. Russell, P. and Hall, D.B. (1983) The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 258, 143-149.
34. +-cotransporter. FEBS Lett. 259, 43-46.
35. Sauer, N., Caspari, T., Klebl, F. and Tanner, W. (1990) Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Proc. Natl Acad. Sci. USA, 87, 7949-7952.
36. Sentenac, H., Bonneaud, N., Minet, M., Lacroute, F., Salmon, J.M., Gaymard, F. and Grignon, C. (1992) Cloning and expression in yeast of a plant potassium ion transport system. Science, 256, 663-665.
37. 2+-ATPases. Nature, 319, 689-693.
38. - symporter. Plant Physiol. 107, 33-41.
39. + symporter in lipid vesicles and purification of the histidine tagged protein from transgenic Saccharomyces cerevisiae. Plant J. 6, 225-233.
40. Stolz, J., Darnhofer-Demar, B. and Sauer, N. (1995) Rapid purification of a functionally active plant sucrose carrier from transgenic yeast using a bacterial biotin acceptor domain. FEBS Lett. 377, 167-171.
41. Tanner, W. (1969) Light-driven active uptake of 3-O-methylglucose via an inducible hexose uptake system of Chlorella. Biochem. Biophys. Res. Commun. 36, 278-386.
42. Tanner, W. and Caspari, T. (1996) Membrane transport carriers. Ann. Rev. Plant. Mol. Biol. 47, 595-625.
43. Viitanen, P., Newman, M.J., Foster, D.L., Wilson, H.T. and Kaback, H.R. (1986) Purification, reconstitution and characterisation of the lac permease of Escherichia coli. Methods Enzymol. 125, 429-452.
44. Glc subunit. FEBS Lett. 324, 109-112.
45. West, I.C. and Mitchell, P. (1973) Stoichometry of lactose-protein symport across the plasma membrane of Escherichia coli. Biochem. J. 132, 587-592.
46. + cotransporter randomly generated by PCR. Proc. Natl Acad. Sci. USA, 91, 10 163-10 167.