Improving sensitivity of the electrophoretic mobility shift assay by restricting tissue phosphatase activities

BioTechniques

Volumn 24, Issue 6, 1998, Pages 964-970

  Laniel, Marc André ^a   Guérin, Sylvain L ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; PHOSPHATASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEPHOSPHORYLATION; ELECTROPHORETIC MOBILITY; KIDNEY; LIVER; MOLECULAR DYNAMICS; NONHUMAN; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; RAT; TEMPERATURE; TIME;

ANIMALIA;

EID: 2642674404     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98246st02     Document Type: Article

References (21)

1. Ausubel, F.M., B. Roger, R.E. Kingston, D.D. Moore, J.G. Seideman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
2. Chen, M., A. Laszlo, J.L.R. Roti and C.R. Hunt. 1996. Detection of single-base mutations by a competitive mobility shift assay. Anal. Biochem. 239:61-69.
3. De Vries, E., W. Van Driel, S.J.L. Van den Heuvel and P.C. Van der Vliet. 1987. Contact analysis of the HeLa nuclear factor 1 recognition site reveal symmetrical binding at one site of the DNA helix. EMBO J. 6:161- 168.
4. Dyer, R.B. and N.K. Herzog. 1995. Immuno- depletion EMSA: a novel method to identify proteins in a protein-DNA complex. Nucleic Acids Res. 23:3345-3346.
5. Dynan, W.S. and R. Tjian. 1983. The promoter-specific transcription factor Spl binds to upstream sequences in the SV40 early promoter. Cell 35:79-87.
6. Fenton, C. and M.R. El-Gewely. 1997. Pyrophosphate in the binding reaction increases the sensitivity of mobility shift analysis. BioTechniques 23:248-250.
7. Graves, B.J., P.F. Johnson and S.L. McKnight. 1986. Homologous recognition of a promoter domain common to the MSV LTR and HSV Tk gene. Cell 44:565-576.
8. Hoshi, K., N. Amizuka, K. Oda, Y. Ikehara and H. Ozawa. 1997. Immunolocalization of tissue non-specific alkaline phosphatase in mice. Histochem. Cell Biol. 107:183-191.
9. Kenter, A.L. and L. Morgan. 1995. Mitogenic activation of a nuclear alkaline phosphatase in normal activated lymphocytes. J. Immunol. 154:2041-2050.
10. Leclerc, S., W. Eskild and S.L. Guérin. 1997. The rat growth hormone and human cellular retinol binding protein 1 genes share homologous NF1-like binding sites that exert either positive or negative influences on gene expression in vitro. DNA Cell Biol. 16:951- 967.
11. Lin, M.F., S.S. Li, T.M. Chu and C.L. Lee. 1990. Comparison of prostate acid phosphatase with acidic phosphatase isoenzymes from the lung and spleen. J. Clin. Lab. Anal. 4:420-425.
12. McComb, R.B., G.N. Bowers and S. Posen. 1979. Alkaline Phosphatase, Plenum Press, New York.
13. Novak, U. and L. Paradiso. 1995. Identification of proteins in DNA-protein complexes after blotting of EMSA gels. BioTechniques 19:54-55.
14. Orchard, K. and G.E. May. 1993. An EMSA-based method for determining the molecular weight of a protein-DNA complex. Nucleic Acids Res. 21:3335-3336.
15. Roy, R.J., P. Gosselin, M.J. Anzívíno, D.D. Moore and S.L. Guérin. 1992. Binding of a nuclear protein to the rat growth hormone silencer element. Nucleic Acids Res. 20:401- 408.
16. Roy, R.J., P. Gosselin and S.L. Guérin. 1991. A short protocol for micro-purification of nuclear proteins from whole animal tissue. BioTechniques 11:770-777.
17. Roy, R.J. and S.L. Guérin. 1994. The 30- kDa rat liver transcription factor nuclear factor 1 binds the rat growth hormone proximal silencer. Eur. J. Biochem. 219:799-806.
18. Vergnes, H.A., A. Brisson-Lougarre, J.G. Grozdea, C.J. Blum, Y. Kihn and J. Sevely. 1992. Nuclear localization and characterization of alkaline phosphatase in neutrophils from normal controls and pregnant women. Am. J. Hematol. 39:249-256.
19. Wilson, M.J. and K. Ahmed. 1976. Presence and androgen control of an alkaline phosphatase in the nucleus of rat ventral prostate. Biochim. Biophys. Acta 429:439-447.
20. Yamamoto, H. 1997. DNA mobility shift assay coupled with SDS-PAGE for detection of DNA-binding proteins. BioTechniques 22:210-211.
21. Zernik, J., B. Kream and K. Twarog. 1991. Tissue-specific and dexamethasone-inducible expression of alkaline phosphatase from alternative promoters of the rat bone/liver/kidney/placenta gene. Biochem. Biophys. Res. Commun. 176:1149-1156.