DNA mobility shift assay coupled with SDS-PAGE for detection of DNA- binding proteins

BioTechniques

Volumn 22, Issue 2, 1997, Pages 210-211

  Yamamoto, Hisanori ^a,b  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN;

ARTICLE; DNA DETERMINATION; DNA PROTEIN COMPLEX; DNA REPLICATION; EPSTEIN BARR VIRUS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN LOCALIZATION;

HUMAN HERPESVIRUS 4;

EID: 0031052856     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm01     Document Type: Article

References (6)

1. Bird, A.G., S.M. McLachlan and S. Britton. 1981. Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature 289:300-301.
2. Carey, J. 1991. Gel retardation. Methods Enzymol. 208:103-117.
3. Inoue, N., S. Harada, T. Honma, T. Kitamura and K. Yanagi. 1991. The domain of Epstein-Barr virus nuclear antigen 1 essential for binding to oriP region has a sequence fitted for the hypothetical basic-helix-loop-helix structure. Virology 182:84-93.
4. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
5. Rawlins, D.R., G. Milman, S.D. Hayward and G.S. Hayward. 1985. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell 42:859-868.
6. Schreiber, E., P. Matthias, M.M. Muller and W. Schaffner. 1989. Rapid detection of octamer binding proteins with "mini-extracts", prepared from a small number of cells. Nucleic Acids Res. 17:6419.